Effect of vitamin E on serum aminotransferase and thioredoxin levels in patients with viral hepatitis C

Sabina Mahmood, Gotaro Yamada, Gouichi Niiyama, Miwa Kawanaka, Kazumi Togawa, Miho Sho, Toshio Ito, Takayo Sasagawa, Misako Okita, Hajime Nakamura, Junji Yodoi

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Objectives: Oxidative stress induces cellular responses such as cell death, gene activation and cell proliferation, in the liver. Vitamin E (Vit. E) has been found to protect the liver against oxidative stress in animal experiments. Thioredoxin (TRX) is a stress inducible, multifunctional protein, secreted during oxidative stress. This study evaluated effects of Vit. E on serum TRX and amino-transferase levels in hepatitis C virus (HCV) patients, partly non-responsive to initial interferon (IFN), with higher than average level of serum alanine aminotransferase (ALT) after receiving anti-inflammatory drug treatment. Methods: Seventeen HCV patients (male = 3; female = 14) of age 62 ± 7.65 years receiving anti-inflammatory drug therapy, at least 6 months prior to Vit. E administration, were given d-́a-tocopherol 500 mg/day, orally, for a period of 3 months. ALT, aspartate aminotransferase (AST), TRX and Vit. E were measured at 0, 1, 2 and 3 months and 1 month after end of treatment. As controls, the same patients biochemical data, 3 months from the start of therapy were used. Patients were divided into three categories: total patients "T", low ALT group "L" (ALT <70 IU/l) and high ALT group "H" (ALT > 70 IU/l), respectively. Results: The ALT level was lowered, significantly in group H, in the 1st, 2nd, 3rd and 1-month post therapy, compared to the initial value. But group L showed little or no change in ALT. Post Vit. E therapy, in groups T and H, the TRX level was elevated but remained below initial levels, whereas in group L, TRX level remained significantly lower than the pretreatment value. Groups T and L, showed significant reduction (p <0.05) in serum TRX levels in the 2nd and 3rd month. Group H showed a tendency towards TRX reduction, but not significantly. Serum Vit. E levels increased significantly (p <0.0001) from the 1st to 3rd month in all three T, H and L groups. Conclusion: Oxidative stress induced liver damage is reduced by Vit. E in patients with viral hepatitis C, particularly those with initial ALT levels > 70 IU/l. Vit. E treatment causes reduction of oxidative stress markers as TRX and ALT in sera. Therefore, Vit. E can act as a supportive therapy to combat liver damage caused by oxidative stress, in such patients with continuously high levels of ALT even after anti-viral and anti-inflammatory drug therapy.

Original languageEnglish
Pages (from-to)781-785
Number of pages5
JournalFree Radical Research
Volume37
Issue number7
DOIs
Publication statusPublished - Jul 1 2003
Externally publishedYes

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Thioredoxins
Hepatitis C
Transaminases
Alanine Transaminase
Vitamin E
Oxidative stress
Oxidative Stress
Serum
Drug therapy
Liver
Anti-Inflammatory Agents
Viruses
Hepacivirus
Therapeutics
Drug Therapy
Tocopherols
Cell proliferation
Cell death
Transferases
Aspartate Aminotransferases

Keywords

  • ALT
  • Thioredoxin
  • Viral hepatitis C oxidative stress
  • Vitamin E

ASJC Scopus subject areas

  • Biochemistry

Cite this

Effect of vitamin E on serum aminotransferase and thioredoxin levels in patients with viral hepatitis C. / Mahmood, Sabina; Yamada, Gotaro; Niiyama, Gouichi; Kawanaka, Miwa; Togawa, Kazumi; Sho, Miho; Ito, Toshio; Sasagawa, Takayo; Okita, Misako; Nakamura, Hajime; Yodoi, Junji.

In: Free Radical Research, Vol. 37, No. 7, 01.07.2003, p. 781-785.

Research output: Contribution to journalArticle

Mahmood, S, Yamada, G, Niiyama, G, Kawanaka, M, Togawa, K, Sho, M, Ito, T, Sasagawa, T, Okita, M, Nakamura, H & Yodoi, J 2003, 'Effect of vitamin E on serum aminotransferase and thioredoxin levels in patients with viral hepatitis C', Free Radical Research, vol. 37, no. 7, pp. 781-785. https://doi.org/10.1080/1071576031000102141
Mahmood, Sabina ; Yamada, Gotaro ; Niiyama, Gouichi ; Kawanaka, Miwa ; Togawa, Kazumi ; Sho, Miho ; Ito, Toshio ; Sasagawa, Takayo ; Okita, Misako ; Nakamura, Hajime ; Yodoi, Junji. / Effect of vitamin E on serum aminotransferase and thioredoxin levels in patients with viral hepatitis C. In: Free Radical Research. 2003 ; Vol. 37, No. 7. pp. 781-785.
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T1 - Effect of vitamin E on serum aminotransferase and thioredoxin levels in patients with viral hepatitis C

AU - Mahmood, Sabina

AU - Yamada, Gotaro

AU - Niiyama, Gouichi

AU - Kawanaka, Miwa

AU - Togawa, Kazumi

AU - Sho, Miho

AU - Ito, Toshio

AU - Sasagawa, Takayo

AU - Okita, Misako

AU - Nakamura, Hajime

AU - Yodoi, Junji

PY - 2003/7/1

Y1 - 2003/7/1

N2 - Objectives: Oxidative stress induces cellular responses such as cell death, gene activation and cell proliferation, in the liver. Vitamin E (Vit. E) has been found to protect the liver against oxidative stress in animal experiments. Thioredoxin (TRX) is a stress inducible, multifunctional protein, secreted during oxidative stress. This study evaluated effects of Vit. E on serum TRX and amino-transferase levels in hepatitis C virus (HCV) patients, partly non-responsive to initial interferon (IFN), with higher than average level of serum alanine aminotransferase (ALT) after receiving anti-inflammatory drug treatment. Methods: Seventeen HCV patients (male = 3; female = 14) of age 62 ± 7.65 years receiving anti-inflammatory drug therapy, at least 6 months prior to Vit. E administration, were given d-́a-tocopherol 500 mg/day, orally, for a period of 3 months. ALT, aspartate aminotransferase (AST), TRX and Vit. E were measured at 0, 1, 2 and 3 months and 1 month after end of treatment. As controls, the same patients biochemical data, 3 months from the start of therapy were used. Patients were divided into three categories: total patients "T", low ALT group "L" (ALT <70 IU/l) and high ALT group "H" (ALT > 70 IU/l), respectively. Results: The ALT level was lowered, significantly in group H, in the 1st, 2nd, 3rd and 1-month post therapy, compared to the initial value. But group L showed little or no change in ALT. Post Vit. E therapy, in groups T and H, the TRX level was elevated but remained below initial levels, whereas in group L, TRX level remained significantly lower than the pretreatment value. Groups T and L, showed significant reduction (p <0.05) in serum TRX levels in the 2nd and 3rd month. Group H showed a tendency towards TRX reduction, but not significantly. Serum Vit. E levels increased significantly (p <0.0001) from the 1st to 3rd month in all three T, H and L groups. Conclusion: Oxidative stress induced liver damage is reduced by Vit. E in patients with viral hepatitis C, particularly those with initial ALT levels > 70 IU/l. Vit. E treatment causes reduction of oxidative stress markers as TRX and ALT in sera. Therefore, Vit. E can act as a supportive therapy to combat liver damage caused by oxidative stress, in such patients with continuously high levels of ALT even after anti-viral and anti-inflammatory drug therapy.

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KW - ALT

KW - Thioredoxin

KW - Viral hepatitis C oxidative stress

KW - Vitamin E

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