Effect of vascular endothelial growth factor on maturation, fertilization and developmental competence of bovine oocytes

Hailing Luo, Koji Kimura, Mari Aoki, Makoto Hirako

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

To examine the effect of Vascular Endothelial Growth Factor (VEGF) on the maturation of bovine oocytes, human recombinant VEGF165 was used in 3 experiments. In Exp. 1, bovine cumulus oocyte complexes (COCs) were matured for 22 hr in modified Synthetic Oviduct Fluid (m-SOF) supplemented with 0 (control) or 5 ng/ml of VEGF. Maturation rate increased (P<0.05) from 78.2% in the control to 90.5% in the VEGF treated group. In Exp. 2, bovine COCs were matured in m-SOF and co-incubated with sperm in modified BO medium, each supplemented with or without 5 ng/ml VEGF. Normal fertilization rate was improved (P<0.05) from 63.0% (control) to 79.8% or 82.3% with VEGF during maturation or both maturation and fertilization. In Exp. 3, bovine COCs were matured the same way as in Exp. 1, then co-incubated with sperm for 6 hr and cultured for 162 hr in m-SOF without VEGF. Cleavage rate and development rate to the 4- to 8-cell stage were examined at 42 hr post-co-incubation and development rate to blastocyst was examined at 162 hr post-co-incubation. Cleavage, the development to the 4- to 8-cell stage and blastocyst rates (82.0%, 70.3% and 45.1%, respectively) were significantly higher (P<0.05) in the VEGF group than those in the control (67.3%, 52.5% and 33.3%, respectively). These results indicate that VEGF has a beneficial effect on the maturation of bovine oocytes.

Original languageEnglish
Pages (from-to)803-806
Number of pages4
JournalJournal of Veterinary Medical Science
Volume64
Issue number9
DOIs
Publication statusPublished - Sep 1 2002
Externally publishedYes

Keywords

  • Bovine oocyte
  • Early embryonic development
  • Fertilization
  • Maturation
  • Vascular Endothelial Growth Factor

ASJC Scopus subject areas

  • veterinary(all)

Fingerprint Dive into the research topics of 'Effect of vascular endothelial growth factor on maturation, fertilization and developmental competence of bovine oocytes'. Together they form a unique fingerprint.

Cite this