Effect of transient TCDD exposure on immortalized human trophoblast-derived cell lines

K. Fukushima, K. Tsukimori, D. Li, T. Takao, S. Morokuma, K. Kato, H. Seki, S. Takeda, S. Matsumura, N. Wake

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Low level, antenatal exposure to dioxins is associated with low birth weight, which in turn is associated with long-term sequelae. We exposed the human extravillous cytotrophoblast (EVT) lines HTR-8/SV40 and TCL1 to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) and assessed cell growth, invasion, and differentiation. TCDD had no effect on cell proliferation, invasion, or tube formation in Matrigel. The EVT-derived cells expressed a functional aryl hydrocarbon receptor protein; however, TCDD exposure did not alter expression levels of proteins involved in EVT differentiation in early pregnancy, including hypoxia-inducible factor 1A (HIF1A), vascular endothelial growth factor (VEGF), Integrin A1, A6, and AVB3. These results suggest that the reduction in fetal weight induced by dioxin is not the result of vascular remodeling via EVT dysfunction.

Original languageEnglish
Pages (from-to)550-556
Number of pages7
JournalHuman and Experimental Toxicology
Volume31
Issue number6
DOIs
Publication statusPublished - Jun 2012

Keywords

  • 2, 3, 7, 8-tetrachlorodibenzo-p-dioxin (TCDD)
  • placenta
  • pregnancy
  • trophoblast

ASJC Scopus subject areas

  • Toxicology
  • Health, Toxicology and Mutagenesis

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    Fukushima, K., Tsukimori, K., Li, D., Takao, T., Morokuma, S., Kato, K., Seki, H., Takeda, S., Matsumura, S., & Wake, N. (2012). Effect of transient TCDD exposure on immortalized human trophoblast-derived cell lines. Human and Experimental Toxicology, 31(6), 550-556. https://doi.org/10.1177/0960327111424305