Effect of sulfide, osmotic, and thermal stresses on taurine transporter mRNA levels in the gills of the hydrothermal vent-specific mussel Bathymodiolus septemdierum

Ikumi Nakamura-Kusakabe, Toshihiro Nagasaki, Azusa Kinjo, Mieko Sassa, Tomoko Koito, Kei Okamura, Shosei Yamagami, Toshiro Yamanaka, Shinji Tsuchida, Koji Inoue

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Hydrothermal vent environmental conditions are characterized by high sulfide concentrations, fluctuating osmolality, and irregular temperature elevations caused by vent effluents. These parameters represent potential stressors for organisms that inhabit the area around hydrothermal vents. Here, we aimed to obtain a better understanding of the adaptation mechanisms of marine species to hydrothermal vent environments. Specifically, we examined the effect of sulfide, osmolality, and thermal stress on the expression of taurine transporter (TAUT) mRNA in the gill of the deep-sea mussel Bathymodiolus septemdierum, which is a dominant species around hydrothermal vent sites. We analyzed TAUT mRNA levels by quantitative real-time polymerase chain reaction (PCR) in the gill of mussels exposed to sulfide (0.1 or 1mg/L Na2S·9H2O), hyper- (115% seawater) and hypo- (97.5%, 95.5%, and 85% seawater) osmotic conditions, and thermal stresses (12°C and 20°C) for 24 and 48h. The results showed that mussels exposed to relatively low levels of sulfide (0.1mg/L) and moderate heat stress (12°C) exhibited higher TAUT mRNA levels than the control. Although hyper- and hypo-osmotic stress did not significantly change TAUT mRNA levels, slight induction was observed in mussels exposed to low osmolality. Our results indicate that TAUT is involved in the coping mechanism of mussels to various hydrothermal vent stresses.

Original languageEnglish
Pages (from-to)74-79
Number of pages6
JournalComparative Biochemistry and Physiology - A Molecular and Integrative Physiology
Volume191
DOIs
Publication statusPublished - Jan 1 2016

Fingerprint

Hydrothermal Vents
Vents
Bivalvia
Osmotic Pressure
Sulfides
Thermal stress
Hot Temperature
Messenger RNA
Osmolar Concentration
Seawater
Oceans and Seas
Polymerase chain reaction
Real-Time Polymerase Chain Reaction
taurine transporter
Effluents
Temperature

Keywords

  • Deep sea
  • Environmental adaptation
  • Hypotaurine
  • Marine invertebrate
  • Osmoregulation
  • Taurine transporter

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Physiology

Cite this

Effect of sulfide, osmotic, and thermal stresses on taurine transporter mRNA levels in the gills of the hydrothermal vent-specific mussel Bathymodiolus septemdierum. / Nakamura-Kusakabe, Ikumi; Nagasaki, Toshihiro; Kinjo, Azusa; Sassa, Mieko; Koito, Tomoko; Okamura, Kei; Yamagami, Shosei; Yamanaka, Toshiro; Tsuchida, Shinji; Inoue, Koji.

In: Comparative Biochemistry and Physiology - A Molecular and Integrative Physiology, Vol. 191, 01.01.2016, p. 74-79.

Research output: Contribution to journalArticle

Nakamura-Kusakabe, Ikumi ; Nagasaki, Toshihiro ; Kinjo, Azusa ; Sassa, Mieko ; Koito, Tomoko ; Okamura, Kei ; Yamagami, Shosei ; Yamanaka, Toshiro ; Tsuchida, Shinji ; Inoue, Koji. / Effect of sulfide, osmotic, and thermal stresses on taurine transporter mRNA levels in the gills of the hydrothermal vent-specific mussel Bathymodiolus septemdierum. In: Comparative Biochemistry and Physiology - A Molecular and Integrative Physiology. 2016 ; Vol. 191. pp. 74-79.
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AU - Nagasaki, Toshihiro

AU - Kinjo, Azusa

AU - Sassa, Mieko

AU - Koito, Tomoko

AU - Okamura, Kei

AU - Yamagami, Shosei

AU - Yamanaka, Toshiro

AU - Tsuchida, Shinji

AU - Inoue, Koji

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