Effect of retinoids on fetal lung development in the rat

Hisashi Masuyama, Yuji Hiramatsu, Takafumi Kudo

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

We investigated the effect of retinoids on fetal lung development in the rat. The concentration of retinyl palmitate increased rapidly to a peak on day 17 of gestation and decreased to a minimum on day 21 of gestation; there was a slight increase after birth. Retinoid acid receptor (RAR)-α and -β mRNA were detected in all samples obtained from perinatal and adult rat lung, and only a trace of RAR-γ mRNA was detected in the fetuses on days 15, 17 and 19 of gestation and in the adults by reverse transcriptase-polymerase chain reaction. After a maternal retinol deficiency of 28 days' duration, fetal body and lung weights were significantly lower than those of controls; the concentrations of retinyl palmitate and phosphatidylcholine (PC) in the lung after a maternal retinol deficiency of 14,21 or 28 days were significantly lower than those of controls. Expression of RAR-β mRNA in the group with 28-day retinol deficiency was lower than in controls, that of RAR-α mRNA was increased and that of RAR-γ mRNA was not influenced by retinol deficiency. The rate of choline incorporation into PC in fetal lung explants was significantly higher in the group treated with retinoic acid (RA) than in controls. RA enhanced the effect of epidermal growth factor on choline incorporation and prevented that of dexamethasone. In conclusion, storage of retinoids occurs and all three types of RAR mRNA are expressed during fetal lung growth and development, and as retinoids exert both a direct and an indirect effect on choline incoporation in fetal rat lung, we think that they may be one of the factors controlling fetal lung development in this species.

Original languageEnglish
Pages (from-to)264-273
Number of pages10
JournalNeonatology
Volume67
Issue number4
DOIs
Publication statusPublished - 1995

Fingerprint

Retinoids
Fetal Development
Lung
Messenger RNA
Acids
Choline
Tretinoin
Phosphatidylcholines
Pregnancy
Mothers
Fetal Weight
Reverse Transcriptase Polymerase Chain Reaction
Growth and Development
Epidermal Growth Factor
Dexamethasone
Fetus
Parturition
Keratomalacia

Keywords

  • Fetal lung development
  • Phosphatidylcholine
  • Retinoid acid receptor
  • Retinol deficiency
  • Retinol Retinoic acid
  • Retinyl palmitate
  • Reverse transcriptase-polymerase chain reaction

ASJC Scopus subject areas

  • Developmental Biology
  • Pediatrics, Perinatology, and Child Health

Cite this

Effect of retinoids on fetal lung development in the rat. / Masuyama, Hisashi; Hiramatsu, Yuji; Kudo, Takafumi.

In: Neonatology, Vol. 67, No. 4, 1995, p. 264-273.

Research output: Contribution to journalArticle

Masuyama, Hisashi ; Hiramatsu, Yuji ; Kudo, Takafumi. / Effect of retinoids on fetal lung development in the rat. In: Neonatology. 1995 ; Vol. 67, No. 4. pp. 264-273.
@article{af5602e9ed3b4d7ea67f656627c98017,
title = "Effect of retinoids on fetal lung development in the rat",
abstract = "We investigated the effect of retinoids on fetal lung development in the rat. The concentration of retinyl palmitate increased rapidly to a peak on day 17 of gestation and decreased to a minimum on day 21 of gestation; there was a slight increase after birth. Retinoid acid receptor (RAR)-α and -β mRNA were detected in all samples obtained from perinatal and adult rat lung, and only a trace of RAR-γ mRNA was detected in the fetuses on days 15, 17 and 19 of gestation and in the adults by reverse transcriptase-polymerase chain reaction. After a maternal retinol deficiency of 28 days' duration, fetal body and lung weights were significantly lower than those of controls; the concentrations of retinyl palmitate and phosphatidylcholine (PC) in the lung after a maternal retinol deficiency of 14,21 or 28 days were significantly lower than those of controls. Expression of RAR-β mRNA in the group with 28-day retinol deficiency was lower than in controls, that of RAR-α mRNA was increased and that of RAR-γ mRNA was not influenced by retinol deficiency. The rate of choline incorporation into PC in fetal lung explants was significantly higher in the group treated with retinoic acid (RA) than in controls. RA enhanced the effect of epidermal growth factor on choline incorporation and prevented that of dexamethasone. In conclusion, storage of retinoids occurs and all three types of RAR mRNA are expressed during fetal lung growth and development, and as retinoids exert both a direct and an indirect effect on choline incoporation in fetal rat lung, we think that they may be one of the factors controlling fetal lung development in this species.",
keywords = "Fetal lung development, Phosphatidylcholine, Retinoid acid receptor, Retinol deficiency, Retinol Retinoic acid, Retinyl palmitate, Reverse transcriptase-polymerase chain reaction",
author = "Hisashi Masuyama and Yuji Hiramatsu and Takafumi Kudo",
year = "1995",
doi = "10.1159/000244174",
language = "English",
volume = "67",
pages = "264--273",
journal = "Neonatology",
issn = "1661-7800",
publisher = "S. Karger AG",
number = "4",

}

TY - JOUR

T1 - Effect of retinoids on fetal lung development in the rat

AU - Masuyama, Hisashi

AU - Hiramatsu, Yuji

AU - Kudo, Takafumi

PY - 1995

Y1 - 1995

N2 - We investigated the effect of retinoids on fetal lung development in the rat. The concentration of retinyl palmitate increased rapidly to a peak on day 17 of gestation and decreased to a minimum on day 21 of gestation; there was a slight increase after birth. Retinoid acid receptor (RAR)-α and -β mRNA were detected in all samples obtained from perinatal and adult rat lung, and only a trace of RAR-γ mRNA was detected in the fetuses on days 15, 17 and 19 of gestation and in the adults by reverse transcriptase-polymerase chain reaction. After a maternal retinol deficiency of 28 days' duration, fetal body and lung weights were significantly lower than those of controls; the concentrations of retinyl palmitate and phosphatidylcholine (PC) in the lung after a maternal retinol deficiency of 14,21 or 28 days were significantly lower than those of controls. Expression of RAR-β mRNA in the group with 28-day retinol deficiency was lower than in controls, that of RAR-α mRNA was increased and that of RAR-γ mRNA was not influenced by retinol deficiency. The rate of choline incorporation into PC in fetal lung explants was significantly higher in the group treated with retinoic acid (RA) than in controls. RA enhanced the effect of epidermal growth factor on choline incorporation and prevented that of dexamethasone. In conclusion, storage of retinoids occurs and all three types of RAR mRNA are expressed during fetal lung growth and development, and as retinoids exert both a direct and an indirect effect on choline incoporation in fetal rat lung, we think that they may be one of the factors controlling fetal lung development in this species.

AB - We investigated the effect of retinoids on fetal lung development in the rat. The concentration of retinyl palmitate increased rapidly to a peak on day 17 of gestation and decreased to a minimum on day 21 of gestation; there was a slight increase after birth. Retinoid acid receptor (RAR)-α and -β mRNA were detected in all samples obtained from perinatal and adult rat lung, and only a trace of RAR-γ mRNA was detected in the fetuses on days 15, 17 and 19 of gestation and in the adults by reverse transcriptase-polymerase chain reaction. After a maternal retinol deficiency of 28 days' duration, fetal body and lung weights were significantly lower than those of controls; the concentrations of retinyl palmitate and phosphatidylcholine (PC) in the lung after a maternal retinol deficiency of 14,21 or 28 days were significantly lower than those of controls. Expression of RAR-β mRNA in the group with 28-day retinol deficiency was lower than in controls, that of RAR-α mRNA was increased and that of RAR-γ mRNA was not influenced by retinol deficiency. The rate of choline incorporation into PC in fetal lung explants was significantly higher in the group treated with retinoic acid (RA) than in controls. RA enhanced the effect of epidermal growth factor on choline incorporation and prevented that of dexamethasone. In conclusion, storage of retinoids occurs and all three types of RAR mRNA are expressed during fetal lung growth and development, and as retinoids exert both a direct and an indirect effect on choline incoporation in fetal rat lung, we think that they may be one of the factors controlling fetal lung development in this species.

KW - Fetal lung development

KW - Phosphatidylcholine

KW - Retinoid acid receptor

KW - Retinol deficiency

KW - Retinol Retinoic acid

KW - Retinyl palmitate

KW - Reverse transcriptase-polymerase chain reaction

UR - http://www.scopus.com/inward/record.url?scp=0029043401&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029043401&partnerID=8YFLogxK

U2 - 10.1159/000244174

DO - 10.1159/000244174

M3 - Article

VL - 67

SP - 264

EP - 273

JO - Neonatology

JF - Neonatology

SN - 1661-7800

IS - 4

ER -