Effect of rebamipide, a novel antiulcer agent, on helicobacter pylori adhesion to gastric epithelial cells

Shunji Hayashi, Toshiro Sugiyama, Ken Ichi Amano, Hiroshi Isogai, Emiko Isogai, Miki Aihara, Mikio Kikuchi, Masahiro Asaka, Kenji Yokota, Keiji Oguma, Nobuhiro Fujii, Yoshikazu Hirai

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Abstract

Helicobacter pylori is a major etiological agent in gastroduodenal disorders. The adhesion of H. pylori to human gastric epithelial cells is the initial step of H. pylori infection. Inhibition of H. pylori adhesion is thus a therapeutic target in the prevention of H. pylori infection. Experiments were performed to evaluate the effect of rebamipide, a novel antiulcer agent, on H. pylori adhesion to gastric epithelial cells. MKN-28 and MKN-45 cells, derived from human gastric carcinomas, were used as target cells. Ten H. pylori strains isolated from patients with chronic gastritis and gastric ulcer were used in the study. We evaluated the effect of rebamipide on H. pylori adhesion to MKN-28 and MKN-45 cells quantitatively using our previously established enzyme-linked immunosorbent assay. The adhesion of H. pylori to MKN-28 and MKN-45 cells was significantly inhibited by pretreatment of these cells with 100 μg of rebamipide per ml. However, the adhesion was not affected by the pretreatment of H. pylori with rebamipide. On the other hand, the viabilities of H. pylori, MKN-28 cells, and MKN-45 cells were not affected by rebamipide. Our studies suggest that rebamipide inhibits the adhesion of H. pylori to gastric epithelial cells.

Original languageEnglish
Pages (from-to)1895-1899
Number of pages5
JournalAntimicrobial Agents and Chemotherapy
Volume42
Issue number8
Publication statusPublished - Aug 1998

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ASJC Scopus subject areas

  • Pharmacology (medical)

Cite this

Hayashi, S., Sugiyama, T., Amano, K. I., Isogai, H., Isogai, E., Aihara, M., Kikuchi, M., Asaka, M., Yokota, K., Oguma, K., Fujii, N., & Hirai, Y. (1998). Effect of rebamipide, a novel antiulcer agent, on helicobacter pylori adhesion to gastric epithelial cells. Antimicrobial Agents and Chemotherapy, 42(8), 1895-1899.