Abstract
The present study investigated the influence of hydrostatic pressure on the expression of interleukin-8 (IL-8) in a human chondrocyte-like cell line (HCS-2/8). The cells were exposed to hydrostatic pressure (1, 5, 10, or 50 MPa) by a special apparatus. Total RNA was extracted, and was analyzed by a polymerase chain reaction method for IL-8 mRNA. IL-8 concentrations in a culture medium were assessed by ELISA. The expression of IL-8 mRNA was enhanced after exposure to 50 MPa of hydrostatic pressure. IL-8 level in a culture supernatant increased following 50 MPa of pressure. Increased IL-8 production in the present study is an important point when we consider the pathology of inflammatory joint diseases, and this point also shows that treatment which reduces mechanical loading could improve, or slow the progression of, joint diseases.
| Original language | English |
|---|---|
| Pages (from-to) | 35-39 |
| Number of pages | 5 |
| Journal | Pathophysiology |
| Volume | 6 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Apr 1999 |
Fingerprint
Keywords
- Chondrocytes
- Hydrostatic pressure
- Interleukin-8
- Osteoarthritis
- Rheumatoid arthritis
ASJC Scopus subject areas
- Pathology and Forensic Medicine
Cite this
Effect of pressure loading on interleukin-8 production in chondrocytes. / Kubo, Toshikazu; Arai, Yuji; Takahashi, Kenji; Ishida, Toshihiro; Fujisawa, Takuo; Takigawa, Masaharu; Imanishi, Jiro; Hirasawa, Yasusuke.
In: Pathophysiology, Vol. 6, No. 1, 04.1999, p. 35-39.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Effect of pressure loading on interleukin-8 production in chondrocytes
AU - Kubo, Toshikazu
AU - Arai, Yuji
AU - Takahashi, Kenji
AU - Ishida, Toshihiro
AU - Fujisawa, Takuo
AU - Takigawa, Masaharu
AU - Imanishi, Jiro
AU - Hirasawa, Yasusuke
PY - 1999/4
Y1 - 1999/4
N2 - The present study investigated the influence of hydrostatic pressure on the expression of interleukin-8 (IL-8) in a human chondrocyte-like cell line (HCS-2/8). The cells were exposed to hydrostatic pressure (1, 5, 10, or 50 MPa) by a special apparatus. Total RNA was extracted, and was analyzed by a polymerase chain reaction method for IL-8 mRNA. IL-8 concentrations in a culture medium were assessed by ELISA. The expression of IL-8 mRNA was enhanced after exposure to 50 MPa of hydrostatic pressure. IL-8 level in a culture supernatant increased following 50 MPa of pressure. Increased IL-8 production in the present study is an important point when we consider the pathology of inflammatory joint diseases, and this point also shows that treatment which reduces mechanical loading could improve, or slow the progression of, joint diseases.
AB - The present study investigated the influence of hydrostatic pressure on the expression of interleukin-8 (IL-8) in a human chondrocyte-like cell line (HCS-2/8). The cells were exposed to hydrostatic pressure (1, 5, 10, or 50 MPa) by a special apparatus. Total RNA was extracted, and was analyzed by a polymerase chain reaction method for IL-8 mRNA. IL-8 concentrations in a culture medium were assessed by ELISA. The expression of IL-8 mRNA was enhanced after exposure to 50 MPa of hydrostatic pressure. IL-8 level in a culture supernatant increased following 50 MPa of pressure. Increased IL-8 production in the present study is an important point when we consider the pathology of inflammatory joint diseases, and this point also shows that treatment which reduces mechanical loading could improve, or slow the progression of, joint diseases.
KW - Chondrocytes
KW - Hydrostatic pressure
KW - Interleukin-8
KW - Osteoarthritis
KW - Rheumatoid arthritis
UR - http://www.scopus.com/inward/record.url?scp=0032981151&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032981151&partnerID=8YFLogxK
U2 - 10.1016/S0928-4680(98)00032-7
DO - 10.1016/S0928-4680(98)00032-7
M3 - Article
AN - SCOPUS:0032981151
VL - 6
SP - 35
EP - 39
JO - Pathophysiology
JF - Pathophysiology
SN - 0928-4680
IS - 1
ER -