Effect of pressure loading on interleukin-8 production in chondrocytes

Toshikazu Kubo; Yuji Arai; Kenji Takahashi; Toshihiro Ishida; Takuo Fujisawa; Masaharu Takigawa; Jiro Imanishi; Yasusuke Hirasawa

doi:10.1016/S0928-4680(98)00032-7

Effect of pressure loading on interleukin-8 production in chondrocytes

Toshikazu Kubo, Yuji Arai, Kenji Takahashi, Toshihiro Ishida, Takuo Fujisawa, Masaharu Takigawa, Jiro Imanishi, Yasusuke Hirasawa

Dental School

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

The present study investigated the influence of hydrostatic pressure on the expression of interleukin-8 (IL-8) in a human chondrocyte-like cell line (HCS-2/8). The cells were exposed to hydrostatic pressure (1, 5, 10, or 50 MPa) by a special apparatus. Total RNA was extracted, and was analyzed by a polymerase chain reaction method for IL-8 mRNA. IL-8 concentrations in a culture medium were assessed by ELISA. The expression of IL-8 mRNA was enhanced after exposure to 50 MPa of hydrostatic pressure. IL-8 level in a culture supernatant increased following 50 MPa of pressure. Increased IL-8 production in the present study is an important point when we consider the pathology of inflammatory joint diseases, and this point also shows that treatment which reduces mechanical loading could improve, or slow the progression of, joint diseases.

Original language	English
Pages (from-to)	35-39
Number of pages	5
Journal	Pathophysiology
Volume	6
Issue number	1
DOIs	https://doi.org/10.1016/S0928-4680(98)00032-7
Publication status	Published - Apr 1999

Keywords

Chondrocytes
Hydrostatic pressure
Interleukin-8
Osteoarthritis
Rheumatoid arthritis

ASJC Scopus subject areas

Pathology and Forensic Medicine
Physiology (medical)

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10.1016/S0928-4680(98)00032-7

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title = "Effect of pressure loading on interleukin-8 production in chondrocytes",

abstract = "The present study investigated the influence of hydrostatic pressure on the expression of interleukin-8 (IL-8) in a human chondrocyte-like cell line (HCS-2/8). The cells were exposed to hydrostatic pressure (1, 5, 10, or 50 MPa) by a special apparatus. Total RNA was extracted, and was analyzed by a polymerase chain reaction method for IL-8 mRNA. IL-8 concentrations in a culture medium were assessed by ELISA. The expression of IL-8 mRNA was enhanced after exposure to 50 MPa of hydrostatic pressure. IL-8 level in a culture supernatant increased following 50 MPa of pressure. Increased IL-8 production in the present study is an important point when we consider the pathology of inflammatory joint diseases, and this point also shows that treatment which reduces mechanical loading could improve, or slow the progression of, joint diseases.",

keywords = "Chondrocytes, Hydrostatic pressure, Interleukin-8, Osteoarthritis, Rheumatoid arthritis",

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AU - Kubo, Toshikazu

AU - Arai, Yuji

AU - Takahashi, Kenji

AU - Ishida, Toshihiro

AU - Fujisawa, Takuo

AU - Takigawa, Masaharu

AU - Imanishi, Jiro

AU - Hirasawa, Yasusuke

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N2 - The present study investigated the influence of hydrostatic pressure on the expression of interleukin-8 (IL-8) in a human chondrocyte-like cell line (HCS-2/8). The cells were exposed to hydrostatic pressure (1, 5, 10, or 50 MPa) by a special apparatus. Total RNA was extracted, and was analyzed by a polymerase chain reaction method for IL-8 mRNA. IL-8 concentrations in a culture medium were assessed by ELISA. The expression of IL-8 mRNA was enhanced after exposure to 50 MPa of hydrostatic pressure. IL-8 level in a culture supernatant increased following 50 MPa of pressure. Increased IL-8 production in the present study is an important point when we consider the pathology of inflammatory joint diseases, and this point also shows that treatment which reduces mechanical loading could improve, or slow the progression of, joint diseases.

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KW - Rheumatoid arthritis

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