Effect of preillumination with red light on photosynthetic parameters and oxidant-/antioxidant balance in Arabidopsis thaliana in response to UV-A

Vladimir D. Kreslavski, Galina N. Shirshikova, Valery Yu Lyubimov, Alexander N. Shmarev, Alexander M. Boutanaev, Anatoly A. Kosobryukhov, Franz Josef Schmitt, Thomas Friedrich, Suleyman Allakhverdiev

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

The effect of preillumination with low intensity (10 μmol quanta m -2 s-1, 10 min) light of different wavelengths in the spectral range of 550-730 nm on photosynthesis and activity of PSII, the content of photosynthetic pigments and H2O2, as well as the peroxidase activity in the leaves of 26-d-old Arabidopsis thaliana wild-type (WT) plants in response to UV-A radiation was studied. UV-A decreased the activity of the PSII, the content of Chl a, Chl b and carotenoids, as well as increased the peroxidase activity and H2O2 level in the WT leaves. Preillumination of the leaves with red light (RL, λ max = 664 nm) reduced the inhibitory effect of UV radiation on photosynthesis and activity of the PSII, indicated by delayed light emission as well as the H2O2 level, but increased the peroxidase activity in the leaves compared to illumination by UV radiation only. Illumination with RL alone and the subsequent exposure of plants to darkness increased the peroxidase activity and the transcription activity of genes of the transcription factors APX1 and HYH. Preillumination of leaves with RL, then far red light (FRL, λmax = 727 nm) partially compensated the effect of the RL for all studied parameters, suggesting that the active form of phytochrome (PFR) is involved in these processes. Preillumination with the wavelengths of 550, 594 and 727 nm only did not have a marked effect on photosynthesis. The hy2 mutant of Arabidopsis with reduced synthesis of the phytochrome B chromophore showed decreased resistance of PSII to UV-A compared with the WT of Arabidopsis. UV radiation reduced Chl a fluorescence much faster in the hy2 mutant compared to the WT. Preillumination of the hy2 mutant with RL did not affect the PSII activity and H2O2 level in UV-irradiated leaves. It is assumed that the formation of the increased resistance of the photosynthetic apparatus of Arabidopsis to UV-A radiation involves PFR and the antioxidant system of plants, partly by inducing transcriptional activity of some antioxidant and transcription factors genes.

Original languageEnglish
Pages (from-to)229-236
Number of pages8
JournalJournal of Photochemistry and Photobiology B: Biology
Volume127
DOIs
Publication statusPublished - Oct 1 2013
Externally publishedYes

Fingerprint

antioxidants
Arabidopsis
Oxidants
Peroxidase
Photosynthesis
Antioxidants
Radiation
Light
leaves
Lighting
Phytochrome B
Transcription Factors
photosynthesis
Phytochrome
phytochrome
Darkness
Radiation Effects
radiation
Carotenoids
Genes

Keywords

  • Arabidopsis thaliana
  • HO
  • Red light illumination
  • Stress-resistance
  • Transcription
  • UV-A

ASJC Scopus subject areas

  • Biophysics
  • Radiation
  • Radiological and Ultrasound Technology
  • Radiology Nuclear Medicine and imaging

Cite this

Effect of preillumination with red light on photosynthetic parameters and oxidant-/antioxidant balance in Arabidopsis thaliana in response to UV-A. / Kreslavski, Vladimir D.; Shirshikova, Galina N.; Lyubimov, Valery Yu; Shmarev, Alexander N.; Boutanaev, Alexander M.; Kosobryukhov, Anatoly A.; Schmitt, Franz Josef; Friedrich, Thomas; Allakhverdiev, Suleyman.

In: Journal of Photochemistry and Photobiology B: Biology, Vol. 127, 01.10.2013, p. 229-236.

Research output: Contribution to journalArticle

Kreslavski, VD, Shirshikova, GN, Lyubimov, VY, Shmarev, AN, Boutanaev, AM, Kosobryukhov, AA, Schmitt, FJ, Friedrich, T & Allakhverdiev, S 2013, 'Effect of preillumination with red light on photosynthetic parameters and oxidant-/antioxidant balance in Arabidopsis thaliana in response to UV-A', Journal of Photochemistry and Photobiology B: Biology, vol. 127, pp. 229-236. https://doi.org/10.1016/j.jphotobiol.2013.08.008
Kreslavski, Vladimir D. ; Shirshikova, Galina N. ; Lyubimov, Valery Yu ; Shmarev, Alexander N. ; Boutanaev, Alexander M. ; Kosobryukhov, Anatoly A. ; Schmitt, Franz Josef ; Friedrich, Thomas ; Allakhverdiev, Suleyman. / Effect of preillumination with red light on photosynthetic parameters and oxidant-/antioxidant balance in Arabidopsis thaliana in response to UV-A. In: Journal of Photochemistry and Photobiology B: Biology. 2013 ; Vol. 127. pp. 229-236.
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