Effect of polyphosphoric acid pre-treatment of titanium on attachment, proliferation, and differentiation of osteoblast-like cells (MC3T3-E1)

Kenji Maekawa, Yasuhiro Yoshida, Atsushi Mine, Bart Van Meerbeek, Kazuomi Suzuki, Takuo Kuboki

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Purpose: To evaluate the effect of polyphosphoric acid (PPA) pre-treatment of titanium (Ti) on the initial attachment, proliferation, and differentiation of mouse osteoblast-like cells (MC3T3-E1). Materials and methods: Adsorption of PPA to Ti was achieved by immersing Ti disks (15 mm in diameter) into 0, 1, and 10 wt% PPA and 10 wt% orthophosphoric acid (OPA) for 24 h. On each pre-treated Ti disk, 5.0 × 104 MC3T3-E1 cells were seeded, and 1, 3, and 5 h later cell attachment was evaluated. Cell proliferation was also determined 1, 3, and 5 days after cell seed. Cell differentiation was evaluated 5, 10, and 15 days after cell seed using osteoblast marker gene expression. Total RNA was purified from each culture and Type-I collagen, alkaline phosphatase, and osteocalcin mRNA expression levels were measured by real-time reverse transcription polymerase chain reaction. Results: Adsorption of PPA or OPA to Ti significantly accelerated initial cell attachment at every time point (P

Original languageEnglish
Pages (from-to)320-325
Number of pages6
JournalClinical Oral Implants Research
Volume19
Issue number3
DOIs
Publication statusPublished - Mar 2008

Fingerprint

Titanium
Osteoblasts
Adsorption
Seeds
Osteocalcin
Collagen Type I
Reverse Transcription
Alkaline Phosphatase
polyphosphoric acid
Cell Differentiation
Cell Proliferation
RNA
Gene Expression
Polymerase Chain Reaction
Messenger RNA

Keywords

  • Cell differentiation
  • Cell proliferation
  • MC3T3-E1 cells
  • Polyphosphoric acid
  • Titanium

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Effect of polyphosphoric acid pre-treatment of titanium on attachment, proliferation, and differentiation of osteoblast-like cells (MC3T3-E1). / Maekawa, Kenji; Yoshida, Yasuhiro; Mine, Atsushi; Van Meerbeek, Bart; Suzuki, Kazuomi; Kuboki, Takuo.

In: Clinical Oral Implants Research, Vol. 19, No. 3, 03.2008, p. 320-325.

Research output: Contribution to journalArticle

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