Abstract
Purpose: To evaluate the effect of polyphosphoric acid (PPA) pre-treatment of titanium (Ti) on the initial attachment, proliferation, and differentiation of mouse osteoblast-like cells (MC3T3-E1). Materials and methods: Adsorption of PPA to Ti was achieved by immersing Ti disks (15 mm in diameter) into 0, 1, and 10 wt% PPA and 10 wt% orthophosphoric acid (OPA) for 24 h. On each pre-treated Ti disk, 5.0 × 104 MC3T3-E1 cells were seeded, and 1, 3, and 5 h later cell attachment was evaluated. Cell proliferation was also determined 1, 3, and 5 days after cell seed. Cell differentiation was evaluated 5, 10, and 15 days after cell seed using osteoblast marker gene expression. Total RNA was purified from each culture and Type-I collagen, alkaline phosphatase, and osteocalcin mRNA expression levels were measured by real-time reverse transcription polymerase chain reaction. Results: Adsorption of PPA or OPA to Ti significantly accelerated initial cell attachment at every time point (P
| Original language | English |
|---|---|
| Pages (from-to) | 320-325 |
| Number of pages | 6 |
| Journal | Clinical Oral Implants Research |
| Volume | 19 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Mar 2008 |
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Keywords
- Cell differentiation
- Cell proliferation
- MC3T3-E1 cells
- Polyphosphoric acid
- Titanium
ASJC Scopus subject areas
- Dentistry(all)
Cite this
Effect of polyphosphoric acid pre-treatment of titanium on attachment, proliferation, and differentiation of osteoblast-like cells (MC3T3-E1). / Maekawa, Kenji; Yoshida, Yasuhiro; Mine, Atsushi; Van Meerbeek, Bart; Suzuki, Kazuomi; Kuboki, Takuo.
In: Clinical Oral Implants Research, Vol. 19, No. 3, 03.2008, p. 320-325.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Effect of polyphosphoric acid pre-treatment of titanium on attachment, proliferation, and differentiation of osteoblast-like cells (MC3T3-E1)
AU - Maekawa, Kenji
AU - Yoshida, Yasuhiro
AU - Mine, Atsushi
AU - Van Meerbeek, Bart
AU - Suzuki, Kazuomi
AU - Kuboki, Takuo
PY - 2008/3
Y1 - 2008/3
N2 - Purpose: To evaluate the effect of polyphosphoric acid (PPA) pre-treatment of titanium (Ti) on the initial attachment, proliferation, and differentiation of mouse osteoblast-like cells (MC3T3-E1). Materials and methods: Adsorption of PPA to Ti was achieved by immersing Ti disks (15 mm in diameter) into 0, 1, and 10 wt% PPA and 10 wt% orthophosphoric acid (OPA) for 24 h. On each pre-treated Ti disk, 5.0 × 104 MC3T3-E1 cells were seeded, and 1, 3, and 5 h later cell attachment was evaluated. Cell proliferation was also determined 1, 3, and 5 days after cell seed. Cell differentiation was evaluated 5, 10, and 15 days after cell seed using osteoblast marker gene expression. Total RNA was purified from each culture and Type-I collagen, alkaline phosphatase, and osteocalcin mRNA expression levels were measured by real-time reverse transcription polymerase chain reaction. Results: Adsorption of PPA or OPA to Ti significantly accelerated initial cell attachment at every time point (P
AB - Purpose: To evaluate the effect of polyphosphoric acid (PPA) pre-treatment of titanium (Ti) on the initial attachment, proliferation, and differentiation of mouse osteoblast-like cells (MC3T3-E1). Materials and methods: Adsorption of PPA to Ti was achieved by immersing Ti disks (15 mm in diameter) into 0, 1, and 10 wt% PPA and 10 wt% orthophosphoric acid (OPA) for 24 h. On each pre-treated Ti disk, 5.0 × 104 MC3T3-E1 cells were seeded, and 1, 3, and 5 h later cell attachment was evaluated. Cell proliferation was also determined 1, 3, and 5 days after cell seed. Cell differentiation was evaluated 5, 10, and 15 days after cell seed using osteoblast marker gene expression. Total RNA was purified from each culture and Type-I collagen, alkaline phosphatase, and osteocalcin mRNA expression levels were measured by real-time reverse transcription polymerase chain reaction. Results: Adsorption of PPA or OPA to Ti significantly accelerated initial cell attachment at every time point (P
KW - Cell differentiation
KW - Cell proliferation
KW - MC3T3-E1 cells
KW - Polyphosphoric acid
KW - Titanium
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UR - http://www.scopus.com/inward/citedby.url?scp=38549103024&partnerID=8YFLogxK
U2 - 10.1111/j.1600-0501.2007.01477.x
DO - 10.1111/j.1600-0501.2007.01477.x
M3 - Article
C2 - 18190561
AN - SCOPUS:38549103024
VL - 19
SP - 320
EP - 325
JO - Clinical Oral Implants Research
JF - Clinical Oral Implants Research
SN - 0905-7161
IS - 3
ER -