Abstract
The kernel pellicles of walnut are rich in ellagitannins with antioxidative activity. A polyphenol-rich extract from walnuts (WP, 45% polyphenol) was prepared and evaluated for its hypolipidemic effect in high fat diet fed mice. Oral administration of WP (100 and 200 mg/kg) significantly reduced liver weight and liver and serum triglycerides (TG). Hepatic β-oxidation in cytosol, including peroxisome, was enhanced by WP (50-200 mg/kg). mαNA expressions of hepatic peroxisome proliferatoractivated receptor (PPAα) α and acyl coenzyme A oxidase (ACOX) 1 were enhanced by WP (50-200 mg/kg). With respect to the hypotriglyceridemic mechanism of WP, it suppressed neither olive oil induced serum TG elevation in mice nor oleic acid induced TG accumulation in HepG2 cells. On the other hand, mαNA expressions of PPAαα, ACOX1, and carnitine palmitoyltransferase (CPT) 1A in HepG2 cells were significantly enhanced by addition of WP (100 μg/mL). Moreover, tellimagrandin I, a polyphenolic constituent in WP, enhanced ACOX1 expression at 1-100 μg/mL. In conclusion, WP was found to possess hypotriglyceridemic activity via enhancement of peroxisomal fatty acid β-oxidation in the liver. These results suggest that tellimagrandin I is involved in the hypotriglyceridemic mechanism of WP.
| Original language | English |
|---|---|
| Pages (from-to) | 1786-1792 |
| Number of pages | 7 |
| Journal | Journal of Agricultural and Food Chemistry |
| Volume | 57 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - Mar 11 2009 |
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ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Chemistry(all)
Cite this
Effect of Polyphenol-αich Extract from Walnut on Diet-Induced Hypertriglyceridemia in Mice via Enhancement of Fatty Acid Oxidation in the Liver. / Shimoda, Hirosh; Tanaka, Junji; Kikuchi, Mitsunori; Fukuda, Toshiyuki; Ito, Hideyuki; Hatano, Tsutomu; Yoshida, Takashi.
In: Journal of Agricultural and Food Chemistry, Vol. 57, No. 5, 11.03.2009, p. 1786-1792.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Effect of Polyphenol-αich Extract from Walnut on Diet-Induced Hypertriglyceridemia in Mice via Enhancement of Fatty Acid Oxidation in the Liver
AU - Shimoda, Hirosh
AU - Tanaka, Junji
AU - Kikuchi, Mitsunori
AU - Fukuda, Toshiyuki
AU - Ito, Hideyuki
AU - Hatano, Tsutomu
AU - Yoshida, Takashi
PY - 2009/3/11
Y1 - 2009/3/11
N2 - The kernel pellicles of walnut are rich in ellagitannins with antioxidative activity. A polyphenol-rich extract from walnuts (WP, 45% polyphenol) was prepared and evaluated for its hypolipidemic effect in high fat diet fed mice. Oral administration of WP (100 and 200 mg/kg) significantly reduced liver weight and liver and serum triglycerides (TG). Hepatic β-oxidation in cytosol, including peroxisome, was enhanced by WP (50-200 mg/kg). mαNA expressions of hepatic peroxisome proliferatoractivated receptor (PPAα) α and acyl coenzyme A oxidase (ACOX) 1 were enhanced by WP (50-200 mg/kg). With respect to the hypotriglyceridemic mechanism of WP, it suppressed neither olive oil induced serum TG elevation in mice nor oleic acid induced TG accumulation in HepG2 cells. On the other hand, mαNA expressions of PPAαα, ACOX1, and carnitine palmitoyltransferase (CPT) 1A in HepG2 cells were significantly enhanced by addition of WP (100 μg/mL). Moreover, tellimagrandin I, a polyphenolic constituent in WP, enhanced ACOX1 expression at 1-100 μg/mL. In conclusion, WP was found to possess hypotriglyceridemic activity via enhancement of peroxisomal fatty acid β-oxidation in the liver. These results suggest that tellimagrandin I is involved in the hypotriglyceridemic mechanism of WP.
AB - The kernel pellicles of walnut are rich in ellagitannins with antioxidative activity. A polyphenol-rich extract from walnuts (WP, 45% polyphenol) was prepared and evaluated for its hypolipidemic effect in high fat diet fed mice. Oral administration of WP (100 and 200 mg/kg) significantly reduced liver weight and liver and serum triglycerides (TG). Hepatic β-oxidation in cytosol, including peroxisome, was enhanced by WP (50-200 mg/kg). mαNA expressions of hepatic peroxisome proliferatoractivated receptor (PPAα) α and acyl coenzyme A oxidase (ACOX) 1 were enhanced by WP (50-200 mg/kg). With respect to the hypotriglyceridemic mechanism of WP, it suppressed neither olive oil induced serum TG elevation in mice nor oleic acid induced TG accumulation in HepG2 cells. On the other hand, mαNA expressions of PPAαα, ACOX1, and carnitine palmitoyltransferase (CPT) 1A in HepG2 cells were significantly enhanced by addition of WP (100 μg/mL). Moreover, tellimagrandin I, a polyphenolic constituent in WP, enhanced ACOX1 expression at 1-100 μg/mL. In conclusion, WP was found to possess hypotriglyceridemic activity via enhancement of peroxisomal fatty acid β-oxidation in the liver. These results suggest that tellimagrandin I is involved in the hypotriglyceridemic mechanism of WP.
UR - http://www.scopus.com/inward/record.url?scp=64549111724&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=64549111724&partnerID=8YFLogxK
U2 - 10.1021/jf803441c
DO - 10.1021/jf803441c
M3 - Article
C2 - 19256553
AN - SCOPUS:64549111724
VL - 57
SP - 1786
EP - 1792
JO - Journal of Agricultural and Food Chemistry
JF - Journal of Agricultural and Food Chemistry
SN - 0021-8561
IS - 5
ER -