Effect of organic solvents on extraction behaviour of cytochrome c using reversed micellar solutions

Tsutomu Ono, Takayuki Miyazaki, Masahiro Goto, Fumiyuki Nakashio

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The effect of organic solvents on the extraction behaviour of cytochrome c has been investigated with reversed micellar solutions. An aliphatic solvent such as hexane, octane or isooctane was suitable for protein extraction operations using the reversed micelles. Organic solvents having a different hydrophobicity show significantly different behaviour in protein extraction, where extraction efficiency appears to be governed by the nature of the interfacial complex that is formed by surfactants and proteins. Solvent effects on the protein extraction are more dominant in the AOT system rather than that in the DOLPA system. Addition of alcohol appears to decrease the activity of proteins in the back-extraction step, although it is effective for recovering proteins from the reversed micellar solutions.

Original languageEnglish
Pages (from-to)1-9
Number of pages9
JournalSolvent Extraction Research and Development
Volume1996
Issue number3
Publication statusPublished - 1996
Externally publishedYes

Fingerprint

Cytochromes c
Organic solvents
Proteins
Micelles
Hexanes
Hydrophobicity
Surface-Active Agents
Hexane
Alcohols
Surface active agents

ASJC Scopus subject areas

  • Chemical Engineering(all)
  • Filtration and Separation
  • Chemistry(all)

Cite this

Effect of organic solvents on extraction behaviour of cytochrome c using reversed micellar solutions. / Ono, Tsutomu; Miyazaki, Takayuki; Goto, Masahiro; Nakashio, Fumiyuki.

In: Solvent Extraction Research and Development, Vol. 1996, No. 3, 1996, p. 1-9.

Research output: Contribution to journalArticle

Ono, Tsutomu ; Miyazaki, Takayuki ; Goto, Masahiro ; Nakashio, Fumiyuki. / Effect of organic solvents on extraction behaviour of cytochrome c using reversed micellar solutions. In: Solvent Extraction Research and Development. 1996 ; Vol. 1996, No. 3. pp. 1-9.
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