Effect of organic modifier on resolution of hydrophobic compounds by micellar electrokinetic chromatography

Takashi Kaneta, Tetsuya Yamashita, Totaro Imasaka

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Three organic modifiers, methanol, acetonitrile, and dimethylformamide, were used as organic modifiers to improve the resolution of hydrophobic compounds in micellar electrokinetic chromatography. The capacity factor decreased with increasing concentration of organic modifier. Acetonitrile and dimethylformamide were more effective modifiers to decrease the capacity factor than methanol. Maximum resolution was obtained when the capacity factor was reduced to about 2. Methanol enhanced the resolution for less hydrophobic compounds, while acetonitrile and dimethylformamide were better modifiers to improve the resolution for strongly hydrophobic compounds.

Original languageEnglish
Pages (from-to)1276-1279
Number of pages4
JournalELECTROPHORESIS
Volume15
Issue number1
DOIs
Publication statusPublished - 1994
Externally publishedYes

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry

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