Effect of Norspermidine and Its Related Triamines on the Cell-Free Polyphenylalanine Synthesizing System from Vibrio parahaemolyticus

Shigeo Yamamoto, Yasuyoshi Koumoto, Satoshi Shikami, Sumi Shinoda

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3 Citations (Scopus)

Abstract

The effect of norspermidine and its structurally related triamines on the cell-free polyphenylalanine synthesizing system from Vibrio parahaemolyticus was examined in connection with the requirement of the system for monovalent cation. In the absence of norspermidine, the maximal incorporation of [14C] phenylalanine into hot trichloroacetic acid insoluble material was observed under ionic conditions of 12 mM Mg2+ and 50 mM NH4. 4+. K+ could partially substitute for NH4, but Na+ could not. The addition of norspermidine to the polyphenylalanine synthetic reaction mixture not only lowered the optimal Mg2+ concentration, but it also stimulated. the polyphenylalanine synthesis up to 2-fold with no significant increase in misin of [14C] leucine. Other triamines having one or two methylene chains more than norspermidine were also effective in eliciting these effects. Furthermore, Na+ could not support the polyphenylalanine synthesis even in the presence of norspermidine and, on the contrary, inhibited the polyphenylalanine synthesis induced by NH4+ regardless of whether norspermidine was present or not. These findings are discussed in comparison with the properties of other bacterial cell-free systems.

Original languageEnglish
Pages (from-to)575-585
Number of pages11
JournalMICROBIOLOGY and IMMUNOLOGY
Volume34
Issue number7
DOIs
Publication statusPublished - 1990

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Virology

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