TY - JOUR
T1 - Effect of Norspermidine and Its Related Triamines on the Cell-Free Polyphenylalanine Synthesizing System from Vibrio parahaemolyticus
AU - Yamamoto, Shigeo
AU - Koumoto, Yasuyoshi
AU - Shikami, Satoshi
AU - Shinoda, Sumi
PY - 1990
Y1 - 1990
N2 - The effect of norspermidine and its structurally related triamines on the cell-free polyphenylalanine synthesizing system from Vibrio parahaemolyticus was examined in connection with the requirement of the system for monovalent cation. In the absence of norspermidine, the maximal incorporation of [14C] phenylalanine into hot trichloroacetic acid insoluble material was observed under ionic conditions of 12 mM Mg2+ and 50 mM NH4. 4+. K+ could partially substitute for NH4, but Na+ could not. The addition of norspermidine to the polyphenylalanine synthetic reaction mixture not only lowered the optimal Mg2+ concentration, but it also stimulated. the polyphenylalanine synthesis up to 2-fold with no significant increase in misin of [14C] leucine. Other triamines having one or two methylene chains more than norspermidine were also effective in eliciting these effects. Furthermore, Na+ could not support the polyphenylalanine synthesis even in the presence of norspermidine and, on the contrary, inhibited the polyphenylalanine synthesis induced by NH4+ regardless of whether norspermidine was present or not. These findings are discussed in comparison with the properties of other bacterial cell-free systems.
AB - The effect of norspermidine and its structurally related triamines on the cell-free polyphenylalanine synthesizing system from Vibrio parahaemolyticus was examined in connection with the requirement of the system for monovalent cation. In the absence of norspermidine, the maximal incorporation of [14C] phenylalanine into hot trichloroacetic acid insoluble material was observed under ionic conditions of 12 mM Mg2+ and 50 mM NH4. 4+. K+ could partially substitute for NH4, but Na+ could not. The addition of norspermidine to the polyphenylalanine synthetic reaction mixture not only lowered the optimal Mg2+ concentration, but it also stimulated. the polyphenylalanine synthesis up to 2-fold with no significant increase in misin of [14C] leucine. Other triamines having one or two methylene chains more than norspermidine were also effective in eliciting these effects. Furthermore, Na+ could not support the polyphenylalanine synthesis even in the presence of norspermidine and, on the contrary, inhibited the polyphenylalanine synthesis induced by NH4+ regardless of whether norspermidine was present or not. These findings are discussed in comparison with the properties of other bacterial cell-free systems.
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U2 - 10.1111/j.1348-0421.1990.tb01034.x
DO - 10.1111/j.1348-0421.1990.tb01034.x
M3 - Article
C2 - 2266881
AN - SCOPUS:0025113926
VL - 34
SP - 575
EP - 585
JO - Microbiology and Immunology
JF - Microbiology and Immunology
SN - 0385-5600
IS - 7
ER -