Effect of norspermidine and its related triamines on the cell-free polyphenylalanine synthesizing system from Vibrio parahaemolyticus

S. Yamamoto, Y. Koumoto, S. Shikama, S. Shinoda

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Abstract

The effect of norspermidine and its structurally related triamines on the cell-free polyphenylalanine synthesizing system from Vibrio parahaemolyticus was examined in connection with the requirement of the system for monovalent cation. In the absence of norsperimidine, the maximal incorporation of [14C] phenylalanine into hot trichloroacetic acid insoluble material was observed under ionic conditions of 12 mm Mg2+ and 50 mm NH4+. K+ could partially substitute for NH4+, but Na+ could not. The addition of norspermidine to the polyphenylalanine synthetic reaction mixture not only lowered the optimal Mg2+ concentration, but it also stimulated the polyphenlalanine synthesis up to 2-fold with no significant increase in misincorporation of [14C] leucine. Other triamines having one or two methylene chains more than norspermidine were also effective in eliciting these effects. Futhermore, Na+ could not support the polyphenylalanine synthesis even in the presence of norspermidine and, on the contrary, inhibited the polyphenylalanine synthesis induced by NH4+ regardless of whether norspermidine was present or not. These findings are discussed in comparison with the properties of other bacterial cell-free systems.

Original languageEnglish
Pages (from-to)575-585
Number of pages11
JournalMicrobiology and Immunology
Volume34
Issue number7
Publication statusPublished - 1990

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ASJC Scopus subject areas

  • Immunology and Microbiology(all)
  • Microbiology (medical)
  • Microbiology

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