Abstract
The effect of norspermidine and its structurally related triamines on the cell-free polyphenylalanine synthesizing system from Vibrio parahaemolyticus was examined in connection with the requirement of the system for monovalent cation. In the absence of norsperimidine, the maximal incorporation of [14C] phenylalanine into hot trichloroacetic acid insoluble material was observed under ionic conditions of 12 mm Mg2+ and 50 mm NH4+. K+ could partially substitute for NH4+, but Na+ could not. The addition of norspermidine to the polyphenylalanine synthetic reaction mixture not only lowered the optimal Mg2+ concentration, but it also stimulated the polyphenlalanine synthesis up to 2-fold with no significant increase in misincorporation of [14C] leucine. Other triamines having one or two methylene chains more than norspermidine were also effective in eliciting these effects. Futhermore, Na+ could not support the polyphenylalanine synthesis even in the presence of norspermidine and, on the contrary, inhibited the polyphenylalanine synthesis induced by NH4+ regardless of whether norspermidine was present or not. These findings are discussed in comparison with the properties of other bacterial cell-free systems.
| Original language | English |
|---|---|
| Pages (from-to) | 575-585 |
| Number of pages | 11 |
| Journal | Microbiology and Immunology |
| Volume | 34 |
| Issue number | 7 |
| Publication status | Published - 1990 |
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ASJC Scopus subject areas
- Immunology and Microbiology(all)
- Microbiology (medical)
- Microbiology
Cite this
Effect of norspermidine and its related triamines on the cell-free polyphenylalanine synthesizing system from Vibrio parahaemolyticus. / Yamamoto, S.; Koumoto, Y.; Shikama, S.; Shinoda, S.
In: Microbiology and Immunology, Vol. 34, No. 7, 1990, p. 575-585.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Effect of norspermidine and its related triamines on the cell-free polyphenylalanine synthesizing system from Vibrio parahaemolyticus
AU - Yamamoto, S.
AU - Koumoto, Y.
AU - Shikama, S.
AU - Shinoda, S.
PY - 1990
Y1 - 1990
N2 - The effect of norspermidine and its structurally related triamines on the cell-free polyphenylalanine synthesizing system from Vibrio parahaemolyticus was examined in connection with the requirement of the system for monovalent cation. In the absence of norsperimidine, the maximal incorporation of [14C] phenylalanine into hot trichloroacetic acid insoluble material was observed under ionic conditions of 12 mm Mg2+ and 50 mm NH4+. K+ could partially substitute for NH4+, but Na+ could not. The addition of norspermidine to the polyphenylalanine synthetic reaction mixture not only lowered the optimal Mg2+ concentration, but it also stimulated the polyphenlalanine synthesis up to 2-fold with no significant increase in misincorporation of [14C] leucine. Other triamines having one or two methylene chains more than norspermidine were also effective in eliciting these effects. Futhermore, Na+ could not support the polyphenylalanine synthesis even in the presence of norspermidine and, on the contrary, inhibited the polyphenylalanine synthesis induced by NH4+ regardless of whether norspermidine was present or not. These findings are discussed in comparison with the properties of other bacterial cell-free systems.
AB - The effect of norspermidine and its structurally related triamines on the cell-free polyphenylalanine synthesizing system from Vibrio parahaemolyticus was examined in connection with the requirement of the system for monovalent cation. In the absence of norsperimidine, the maximal incorporation of [14C] phenylalanine into hot trichloroacetic acid insoluble material was observed under ionic conditions of 12 mm Mg2+ and 50 mm NH4+. K+ could partially substitute for NH4+, but Na+ could not. The addition of norspermidine to the polyphenylalanine synthetic reaction mixture not only lowered the optimal Mg2+ concentration, but it also stimulated the polyphenlalanine synthesis up to 2-fold with no significant increase in misincorporation of [14C] leucine. Other triamines having one or two methylene chains more than norspermidine were also effective in eliciting these effects. Futhermore, Na+ could not support the polyphenylalanine synthesis even in the presence of norspermidine and, on the contrary, inhibited the polyphenylalanine synthesis induced by NH4+ regardless of whether norspermidine was present or not. These findings are discussed in comparison with the properties of other bacterial cell-free systems.
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M3 - Article
C2 - 2266881
AN - SCOPUS:0025113926
VL - 34
SP - 575
EP - 585
JO - Microbiology and Immunology
JF - Microbiology and Immunology
SN - 0385-5600
IS - 7
ER -