Effect of nitric oxide synthase inhibitors on bone metabolism in growing rats

Hirokazu Tsukahara, M. Miura, S. Tsuchida, I. Hata, K. Hata, K. Yamamoto, Y. Ishii, I. Muramatsu, M. Sudo

Research output: Contribution to journalArticle

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Abstract

We examined the effects of chronic nitric oxide (NO) blockade on bone mineral status in growing rats. Oral administration of N(G)-nitro-L-arginine methyl ester (L-NAME) for 4 wk caused hypertension and a significant reduction in urinary NO2 and NO3/- excretion. Four-week oral aminoguanidine (AG, 400 mg/dl of drinking water) did not alter blood pressure but caused a significant decrease in urinary NO2/- and NO3/-. Rats treated with L-NAME at doses of 20 and 50 mg/dl had normal bone mineral mass in the lumbar spine, but the highest dose (80 mg/dl) caused a slight decrease in bone mass. Chronic AG induced a significant spine osteopenia. This effect of AG was abolished by the simultaneous administration of L-arginine (2.0 g/dl). AG-induced osteopenia was associated with a significant increase in urine excretion of collagen cross-links with normal serum osteocalcin. These findings indicate that chronic AG administration can cause an imbalance between bone resorption and formation, resulting in a decrease in bone mass in growing rats, and suggest that NO produced by inducible NO synthase plays an important role in basal osteoclast bone degradation activity in vivo.

Original languageEnglish
JournalAmerican Journal of Physiology - Endocrinology and Metabolism
Volume270
Issue number5 33-5
Publication statusPublished - 1996
Externally publishedYes

Fingerprint

Metabolism
Nitric Oxide Synthase
Rats
Bone
NG-Nitroarginine Methyl Ester
Bone and Bones
Metabolic Bone Diseases
Minerals
Nitric Oxide
Spine
Osteocalcin
Nitric Oxide Synthase Type II
Osteoclasts
Bone Resorption
Osteogenesis
Drinking Water
Blood pressure
Oral Administration
Arginine
Collagen

Keywords

  • aminoguanidine
  • biochemical markers
  • dual-energy X-ray absorptiometry
  • osteopenia

ASJC Scopus subject areas

  • Physiology
  • Endocrinology
  • Biochemistry
  • Physiology (medical)

Cite this

Effect of nitric oxide synthase inhibitors on bone metabolism in growing rats. / Tsukahara, Hirokazu; Miura, M.; Tsuchida, S.; Hata, I.; Hata, K.; Yamamoto, K.; Ishii, Y.; Muramatsu, I.; Sudo, M.

In: American Journal of Physiology - Endocrinology and Metabolism, Vol. 270, No. 5 33-5, 1996.

Research output: Contribution to journalArticle

Tsukahara, H, Miura, M, Tsuchida, S, Hata, I, Hata, K, Yamamoto, K, Ishii, Y, Muramatsu, I & Sudo, M 1996, 'Effect of nitric oxide synthase inhibitors on bone metabolism in growing rats', American Journal of Physiology - Endocrinology and Metabolism, vol. 270, no. 5 33-5.
Tsukahara, Hirokazu ; Miura, M. ; Tsuchida, S. ; Hata, I. ; Hata, K. ; Yamamoto, K. ; Ishii, Y. ; Muramatsu, I. ; Sudo, M. / Effect of nitric oxide synthase inhibitors on bone metabolism in growing rats. In: American Journal of Physiology - Endocrinology and Metabolism. 1996 ; Vol. 270, No. 5 33-5.
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