Effect of nicomol on high density lipoprotein (HDL) subfractions, HDL(e)2 and HDL(e)3 separated by electrophoresis

S. Ikeda, H. Nagashima, K. Taketa, M. Watanabe

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High density lipoprotein (HDL) fractions were separated by dextran sulfate-Mg++ from sera of 5 healthy males before and after oral administration of a daily 1200 mg dose of Nicomol (2,2,6,6-tetrakis (nicotinoyloxymethyl) cyclohexanol; Cholexamin, Kyorin Pharmaceutical Co. Ltd.) for 2 weeks. The HDL fractions were further subdivided into HDLe2 and HDLe3 by Utermann's electrophoretic method on polyacrylamide gel containing lauric acid. These subfractions roughly corresponded to HDL2 and HDL3, respectively, which were separated by ultracentrifugation. Nicomol treatment increased the HDLe2/HDLe3 ratio as is observed for the HLD2/HDL3 ratio, in addition to the increase in total HDL cholesterol concentration. The electrophoretic determination of HDLe2/HDLe3 ratio in combination with HDL cholesterol level seemed to be clinically important in assessment of the alteration of HDL2 and HDL3 under physiological and pathological conditions.

Original languageEnglish
Pages (from-to)143-148
Number of pages6
JournalActa medica Okayama
Issue number2
Publication statusPublished - Dec 1 1981

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)


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