Effect of naphthalene on photosystem 2 photochemical activity of pea plants

A. V. Lankin, V. D. Kreslavski, A. Yu Khudyakova, S. K. Zharmukhamedov, Suleyman Allakhverdiev

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The effect of a typical polyaromatic hydrocarbon, naphthalene (Naph), on photosystem 2 (PS-2) photochemical activity in thylakoid membrane preparations and 20-day-old pea leaves was studied. Samples were incubated in water in the presence of Naph (0.078, 0.21, and 0.78 mM) for 0.5-24 h under white light illumination (15 μmol photons·m-2·s-1). The PS-2 activity was determined by studying fast and delayed chlorophyll (Chl) a fluorescence. Incubation of samples in water solutions at Naph concentrations of 0.21 and 0.78 mM led to a decrease in the maximum PS-2 quantum efficiency (Fv/Fm), noticeable changes in the polyphasic induction kinetics of fluorescence (OJIP), and a decrease in the amplitudes of the fast and slow components of delayed fluorescence of Chl a. The rate of release of electrolytes from leaves that were preliminarily incubated with Naph (0.21 mM) was also increased. Significant decrease in the fluorescence parameters in thylakoid membrane preparations was observed at Naph concentration of 0.03 mM and 12-min exposure of the samples. Chlorophyll (a and b) and carotenoid content (mg per gram wet mass) was insignificantly changed. The quantum yields of electron transfer from QA to QB (υET2o) and also to the PS-1 acceptors (φRE1o) were reduced. These results are explained by the increase in the number of QB-non-reducing centers of PS-2, which increased with increasing Naph concentration and exposure time of leaves in Naph solution. The suppression of PS-2 activity was partly abolished in the presence of the electron donor sodium ascorbate. Based on these results, it is suggested that Naph distorts cell membrane intactness and acts mainly on the PS- 2 acceptor and to a lesser degree on the PS-2 donor side.

Original languageEnglish
Pages (from-to)1216-1225
Number of pages10
JournalBiochemistry (Moscow)
Volume79
Issue number11
DOIs
Publication statusPublished - Jan 1 2014
Externally publishedYes

Fingerprint

Peas
Fluorescence
Thylakoids
Electrons
Membranes
naphthalene
Water
Quantum yield
Carotenoids
Cell membranes
Hydrocarbons
Lighting
Quantum efficiency
Electrolytes
Ascorbic Acid
Cell Membrane
Light
Kinetics

Keywords

  • Detached pea leaves
  • Naphthalene
  • Photosynthetic pigments
  • Photosystem 2
  • Thylakoid membranes
  • Variable and delayed fluorescence of chlorophyll

ASJC Scopus subject areas

  • Biochemistry

Cite this

Lankin, A. V., Kreslavski, V. D., Khudyakova, A. Y., Zharmukhamedov, S. K., & Allakhverdiev, S. (2014). Effect of naphthalene on photosystem 2 photochemical activity of pea plants. Biochemistry (Moscow), 79(11), 1216-1225. https://doi.org/10.1134/S0006297914110091

Effect of naphthalene on photosystem 2 photochemical activity of pea plants. / Lankin, A. V.; Kreslavski, V. D.; Khudyakova, A. Yu; Zharmukhamedov, S. K.; Allakhverdiev, Suleyman.

In: Biochemistry (Moscow), Vol. 79, No. 11, 01.01.2014, p. 1216-1225.

Research output: Contribution to journalArticle

Lankin, AV, Kreslavski, VD, Khudyakova, AY, Zharmukhamedov, SK & Allakhverdiev, S 2014, 'Effect of naphthalene on photosystem 2 photochemical activity of pea plants', Biochemistry (Moscow), vol. 79, no. 11, pp. 1216-1225. https://doi.org/10.1134/S0006297914110091
Lankin AV, Kreslavski VD, Khudyakova AY, Zharmukhamedov SK, Allakhverdiev S. Effect of naphthalene on photosystem 2 photochemical activity of pea plants. Biochemistry (Moscow). 2014 Jan 1;79(11):1216-1225. https://doi.org/10.1134/S0006297914110091
Lankin, A. V. ; Kreslavski, V. D. ; Khudyakova, A. Yu ; Zharmukhamedov, S. K. ; Allakhverdiev, Suleyman. / Effect of naphthalene on photosystem 2 photochemical activity of pea plants. In: Biochemistry (Moscow). 2014 ; Vol. 79, No. 11. pp. 1216-1225.
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