Effect of Mycobacterium bovis BCG Vaccination on Mycobacterium-Specific Cellular Proliferation and Tumor Necrosis Factor Alpha Production from Distinct Guinea Pig Leukocyte Populations

Todd M. Lasco, Toshiko Yamamoto, Teizo Yoshimura, Shannon Sedberry Allen, Lynne Cassone, David N. McMurray

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

In this study, we focused on three leukocyte-rich guinea pig cell populations, bronchoalveolar lavage (BAL) cells, resident peritoneal cells (PC), and splenocytes (SPC). BAL cells, SPC, and PC were stimulated either with live attenuated Mycobacterium tuberculosis H37Ra or with live or heat-killed virulent M. tuberculosis H37Rv (multiplicity of infection of 1:100). Each cell population was determined to proliferate in response to heat-killed virulent H37Rv, whereas no measurable proliferative response could be detected upon stimulation with live mycobacteria. Additionally, this proliferative capacity (in SPC and PC populations) was significantly enhanced upon prior vaccination with Mycobacterium bovis BCG. Accordingly, in a parallel set of experiments we found a strong positive correlation between production of antigen-specific bioactive tumor necrosis factor alpha (TNF-α) and prior vaccination with BCG. A nonspecific stimulus, lipopolysaccharide, failed to induce this effect on BAL cells, SPC, and PC. These results showed that production of bioactive TNF-α from mycobacterium-stimulated guinea pig cell cultures positively correlates with the vaccination status of the host and with the virulence of the mycobacterial strain.

Original languageEnglish
Pages (from-to)7035-7042
Number of pages8
JournalInfection and Immunity
Volume71
Issue number12
DOIs
Publication statusPublished - Dec 2003
Externally publishedYes

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Mycobacterium
Mycobacterium bovis
Guinea Pigs
Vaccination
Leukocytes
Tumor Necrosis Factor-alpha
Cell Proliferation
Population
Bronchoalveolar Lavage
Mycobacterium tuberculosis
Hot Temperature
Virulence
Lipopolysaccharides
Cell Culture Techniques
Antigens
Infection

ASJC Scopus subject areas

  • Immunology

Cite this

Effect of Mycobacterium bovis BCG Vaccination on Mycobacterium-Specific Cellular Proliferation and Tumor Necrosis Factor Alpha Production from Distinct Guinea Pig Leukocyte Populations. / Lasco, Todd M.; Yamamoto, Toshiko; Yoshimura, Teizo; Allen, Shannon Sedberry; Cassone, Lynne; McMurray, David N.

In: Infection and Immunity, Vol. 71, No. 12, 12.2003, p. 7035-7042.

Research output: Contribution to journalArticle

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