Abstract
In this study, we focused on three leukocyte-rich guinea pig cell populations, bronchoalveolar lavage (BAL) cells, resident peritoneal cells (PC), and splenocytes (SPC). BAL cells, SPC, and PC were stimulated either with live attenuated Mycobacterium tuberculosis H37Ra or with live or heat-killed virulent M. tuberculosis H37Rv (multiplicity of infection of 1:100). Each cell population was determined to proliferate in response to heat-killed virulent H37Rv, whereas no measurable proliferative response could be detected upon stimulation with live mycobacteria. Additionally, this proliferative capacity (in SPC and PC populations) was significantly enhanced upon prior vaccination with Mycobacterium bovis BCG. Accordingly, in a parallel set of experiments we found a strong positive correlation between production of antigen-specific bioactive tumor necrosis factor alpha (TNF-α) and prior vaccination with BCG. A nonspecific stimulus, lipopolysaccharide, failed to induce this effect on BAL cells, SPC, and PC. These results showed that production of bioactive TNF-α from mycobacterium-stimulated guinea pig cell cultures positively correlates with the vaccination status of the host and with the virulence of the mycobacterial strain.
| Original language | English |
|---|---|
| Pages (from-to) | 7035-7042 |
| Number of pages | 8 |
| Journal | Infection and Immunity |
| Volume | 71 |
| Issue number | 12 |
| DOIs | |
| Publication status | Published - Dec 2003 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Immunology
Cite this
Effect of Mycobacterium bovis BCG Vaccination on Mycobacterium-Specific Cellular Proliferation and Tumor Necrosis Factor Alpha Production from Distinct Guinea Pig Leukocyte Populations. / Lasco, Todd M.; Yamamoto, Toshiko; Yoshimura, Teizo; Allen, Shannon Sedberry; Cassone, Lynne; McMurray, David N.
In: Infection and Immunity, Vol. 71, No. 12, 12.2003, p. 7035-7042.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Effect of Mycobacterium bovis BCG Vaccination on Mycobacterium-Specific Cellular Proliferation and Tumor Necrosis Factor Alpha Production from Distinct Guinea Pig Leukocyte Populations
AU - Lasco, Todd M.
AU - Yamamoto, Toshiko
AU - Yoshimura, Teizo
AU - Allen, Shannon Sedberry
AU - Cassone, Lynne
AU - McMurray, David N.
PY - 2003/12
Y1 - 2003/12
N2 - In this study, we focused on three leukocyte-rich guinea pig cell populations, bronchoalveolar lavage (BAL) cells, resident peritoneal cells (PC), and splenocytes (SPC). BAL cells, SPC, and PC were stimulated either with live attenuated Mycobacterium tuberculosis H37Ra or with live or heat-killed virulent M. tuberculosis H37Rv (multiplicity of infection of 1:100). Each cell population was determined to proliferate in response to heat-killed virulent H37Rv, whereas no measurable proliferative response could be detected upon stimulation with live mycobacteria. Additionally, this proliferative capacity (in SPC and PC populations) was significantly enhanced upon prior vaccination with Mycobacterium bovis BCG. Accordingly, in a parallel set of experiments we found a strong positive correlation between production of antigen-specific bioactive tumor necrosis factor alpha (TNF-α) and prior vaccination with BCG. A nonspecific stimulus, lipopolysaccharide, failed to induce this effect on BAL cells, SPC, and PC. These results showed that production of bioactive TNF-α from mycobacterium-stimulated guinea pig cell cultures positively correlates with the vaccination status of the host and with the virulence of the mycobacterial strain.
AB - In this study, we focused on three leukocyte-rich guinea pig cell populations, bronchoalveolar lavage (BAL) cells, resident peritoneal cells (PC), and splenocytes (SPC). BAL cells, SPC, and PC were stimulated either with live attenuated Mycobacterium tuberculosis H37Ra or with live or heat-killed virulent M. tuberculosis H37Rv (multiplicity of infection of 1:100). Each cell population was determined to proliferate in response to heat-killed virulent H37Rv, whereas no measurable proliferative response could be detected upon stimulation with live mycobacteria. Additionally, this proliferative capacity (in SPC and PC populations) was significantly enhanced upon prior vaccination with Mycobacterium bovis BCG. Accordingly, in a parallel set of experiments we found a strong positive correlation between production of antigen-specific bioactive tumor necrosis factor alpha (TNF-α) and prior vaccination with BCG. A nonspecific stimulus, lipopolysaccharide, failed to induce this effect on BAL cells, SPC, and PC. These results showed that production of bioactive TNF-α from mycobacterium-stimulated guinea pig cell cultures positively correlates with the vaccination status of the host and with the virulence of the mycobacterial strain.
UR - http://www.scopus.com/inward/record.url?scp=0344420138&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0344420138&partnerID=8YFLogxK
U2 - 10.1128/IAI.71.12.7035-7042.2003
DO - 10.1128/IAI.71.12.7035-7042.2003
M3 - Article
C2 - 14638793
AN - SCOPUS:0344420138
VL - 71
SP - 7035
EP - 7042
JO - Infection and Immunity
JF - Infection and Immunity
SN - 0019-9567
IS - 12
ER -