Effect of Mycobacterium bovis BCG vaccination on interleukin-1β and RANTES mRNA expression in guinea pig cells exposed to attenuated and virulent mycobacteria

Amminikutty Jeevan, Teizo Yoshimura, Gregory Foster, David N. McMurray

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

The effect of Mycobacterium bovis BCG vaccination on interleukin-1β (IL-1β) or regulated-upon-activation, normally T-cell-expressed and -secreted chemokine (RANTES) mRNA expression in guinea pig spleen cells stimulated with concanavalin A, lipopolysaccharide (LPS), phorbol myristate acetate (PMA) plus ionomycin, or purified protein derivative (PPD) was studied in vitro. Similarly, peritoneal exudate cell-derived macrophages from naïve and BCG-vaccinated guinea pigs were infected with M. bovis BCG, Mycobacterium avium, the attenuated Mycobacterium tuberculosis H37Ra strain, or virulent strains H37Rv and Erdman of M. tuberculosis. Total RNA was subjected to Northern blot analysis using probes generated from guinea pig IL-1β or RANTES cDNA. Although IL-1β and RANTES mRNA could be detected in the spleen cells from naive animals stimulated with LPS or PMA plus ionomycin, the levels were significantly enhanced after BCG vaccination. mRNA expression was also elevated in macrophages infected with live mycobacteria after BCG vaccination. However, macrophages infected with the virulent H37Rv strain of M. tuberculosis showed 75 to 90% reductions in IL-1β expression and 25 to 60% reductions in RANTES mRNA expression compared with macrophages infected with the attenuated H37Ra strain. The IL-1β mRNA levels peaked as soon as 1 h after PPD stimulation and 4 h after M. tuberculosis H37Rv infection of macrophages. In contrast, RANTES mRNA expression was delayed until 48 h after infection. These results indicate that molecular mediators produced in response to various stimuli associated with protective immunity against mycobacteria are upregulated after BCG vaccination; however, a significantly weaker response was observed with virulent M. tuberculosis. These initial studies indicate that BCG vaccination has a positive effect on IL-1β and RANTES mRNA expression by host cells in a highly relevant animal tuberculosis model.

Original languageEnglish
Pages (from-to)1245-1253
Number of pages9
JournalInfection and Immunity
Volume70
Issue number3
DOIs
Publication statusPublished - 2002
Externally publishedYes

Fingerprint

Mycobacterium
Mycobacterium bovis
Interleukin-1
Chemokines
Guinea Pigs
Vaccination
T-Lymphocytes
Messenger RNA
Mycobacterium tuberculosis
Macrophages
Ionomycin
Tetradecanoylphorbol Acetate
Lipopolysaccharides
Spleen
Mycobacterium avium
Interleukin-17
Exudates and Transudates
Concanavalin A
Infection
Northern Blotting

ASJC Scopus subject areas

  • Immunology

Cite this

Effect of Mycobacterium bovis BCG vaccination on interleukin-1β and RANTES mRNA expression in guinea pig cells exposed to attenuated and virulent mycobacteria. / Jeevan, Amminikutty; Yoshimura, Teizo; Foster, Gregory; McMurray, David N.

In: Infection and Immunity, Vol. 70, No. 3, 2002, p. 1245-1253.

Research output: Contribution to journalArticle

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title = "Effect of Mycobacterium bovis BCG vaccination on interleukin-1β and RANTES mRNA expression in guinea pig cells exposed to attenuated and virulent mycobacteria",
abstract = "The effect of Mycobacterium bovis BCG vaccination on interleukin-1β (IL-1β) or regulated-upon-activation, normally T-cell-expressed and -secreted chemokine (RANTES) mRNA expression in guinea pig spleen cells stimulated with concanavalin A, lipopolysaccharide (LPS), phorbol myristate acetate (PMA) plus ionomycin, or purified protein derivative (PPD) was studied in vitro. Similarly, peritoneal exudate cell-derived macrophages from na{\"i}ve and BCG-vaccinated guinea pigs were infected with M. bovis BCG, Mycobacterium avium, the attenuated Mycobacterium tuberculosis H37Ra strain, or virulent strains H37Rv and Erdman of M. tuberculosis. Total RNA was subjected to Northern blot analysis using probes generated from guinea pig IL-1β or RANTES cDNA. Although IL-1β and RANTES mRNA could be detected in the spleen cells from naive animals stimulated with LPS or PMA plus ionomycin, the levels were significantly enhanced after BCG vaccination. mRNA expression was also elevated in macrophages infected with live mycobacteria after BCG vaccination. However, macrophages infected with the virulent H37Rv strain of M. tuberculosis showed 75 to 90{\%} reductions in IL-1β expression and 25 to 60{\%} reductions in RANTES mRNA expression compared with macrophages infected with the attenuated H37Ra strain. The IL-1β mRNA levels peaked as soon as 1 h after PPD stimulation and 4 h after M. tuberculosis H37Rv infection of macrophages. In contrast, RANTES mRNA expression was delayed until 48 h after infection. These results indicate that molecular mediators produced in response to various stimuli associated with protective immunity against mycobacteria are upregulated after BCG vaccination; however, a significantly weaker response was observed with virulent M. tuberculosis. These initial studies indicate that BCG vaccination has a positive effect on IL-1β and RANTES mRNA expression by host cells in a highly relevant animal tuberculosis model.",
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