Effect of minocycline on induced glial activation by experimental tooth movement

Toru Deguchi, Rie Adachi, Hiroshi Kamioka, Do Gyoon Kim, Henry W. Fields, Teruko Takano-Yamamoto, Hiroyuki Ichikawa, Takashi Yamashiro

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Introduction Orthodontic tooth movement causes pain to a patient. Glial cells are nonneuronal cells in the central nervous system and are implicated in various types of pain. In this study, we assessed glial activation responses after experimental tooth movement using immunocytochemical detection of anti-CD11b (OX42) and glial fibrillary acidic protein immunoreactivity to illustrate the microglial and astrocytes response, respectively. In addition, the effect of minocycline in reducing pain during tooth movement was also investigated. Methods Fifty-five Sprague Dawley rats with and without administration of minocycline after 1, 3, 5, 7, and 14 days (n = 5, for each) of tooth movement were used. Immunohistochemistry for microglia (OX42) and astrocyte (glial fibrillary acidic protein) were performed at the medullary dorsal horn (trigeminal subnucleus caudalis). Three-dimensional quantitative analysis was performed with a confocal fluorescence microscope and a software program. Results There was a significant increase in the OX42 and glial fibrillary acidic protein immunoreactivity in response to tooth movement in the medullary dorsal horn. Furthermore, systematic administration of minocycline, a selective inhibitor of microglial activation, significantly attenuated the nociceptive c-Fos expression in the medullary dorsal horn that was induced by experimental tooth movement. Conclusions These data indicate the possible importance of microglial activation in the development of orthodontic pain. This is also the first report on the systematic application of minocycline.

Original languageEnglish
Pages (from-to)881-888
Number of pages8
JournalAmerican Journal of Orthodontics and Dentofacial Orthopedics
Volume149
Issue number6
DOIs
Publication statusPublished - Jun 1 2016

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Tooth Movement Techniques
Minocycline
Neuroglia
Glial Fibrillary Acidic Protein
Pain
Astrocytes
Microglia
Orthodontics
Sprague Dawley Rats
Software
Central Nervous System
Fluorescence
Immunohistochemistry
Spinal Cord Dorsal Horn

ASJC Scopus subject areas

  • Orthodontics

Cite this

Effect of minocycline on induced glial activation by experimental tooth movement. / Deguchi, Toru; Adachi, Rie; Kamioka, Hiroshi; Kim, Do Gyoon; Fields, Henry W.; Takano-Yamamoto, Teruko; Ichikawa, Hiroyuki; Yamashiro, Takashi.

In: American Journal of Orthodontics and Dentofacial Orthopedics, Vol. 149, No. 6, 01.06.2016, p. 881-888.

Research output: Contribution to journalArticle

Deguchi, T, Adachi, R, Kamioka, H, Kim, DG, Fields, HW, Takano-Yamamoto, T, Ichikawa, H & Yamashiro, T 2016, 'Effect of minocycline on induced glial activation by experimental tooth movement', American Journal of Orthodontics and Dentofacial Orthopedics, vol. 149, no. 6, pp. 881-888. https://doi.org/10.1016/j.ajodo.2015.11.030
Deguchi, Toru ; Adachi, Rie ; Kamioka, Hiroshi ; Kim, Do Gyoon ; Fields, Henry W. ; Takano-Yamamoto, Teruko ; Ichikawa, Hiroyuki ; Yamashiro, Takashi. / Effect of minocycline on induced glial activation by experimental tooth movement. In: American Journal of Orthodontics and Dentofacial Orthopedics. 2016 ; Vol. 149, No. 6. pp. 881-888.
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