Abstract
Midazolam, a benzodiazepine, has an hypnotic effect via benzodiazepine receptors and is widely used as an anaesthetic. Recently, it has been suggested that benzodiazepines modulate cytokine responses. The purpose of the present study was to evaluate the effect of midazolam on interleukin-6 (IL-6) response by observing mRNA expression levels in human peripheral blood mononuclear cells (PBMCs) in the absence of lipopolysaccharide (LPS). PBMCs were isolated from healthy volunteers in endotoxin-free 0.9% sodium chloride solution. The cells were incubated for 2 h at 37°C immediately after isolation. IL-6 mRNA expression levels in the cells were quantified using reverse transcription and competitive polymerase chain reaction. It was found that midazolam time-dependently inhibited the IL-6 mRNA expression in PBMCs in the absence of LPS, and significantly inhibited the IL-6 mRNA expression at 1 μg/ml (P
| Original language | English |
|---|---|
| Pages (from-to) | 320-327 |
| Number of pages | 8 |
| Journal | Cytokine |
| Volume | 15 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - Sep 21 2001 |
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Keywords
- Benzodiazepine receptors
- Human peripheral blood mononuclear cells
- Interleukin-6
- Midazolam
- mRNA
ASJC Scopus subject areas
- Endocrinology
- Molecular Biology
- Immunology
- Immunology and Allergy
Cite this
Effect of midazolam on interleukin-6 mRNA expression in human peripheral blood mononuclear cells in the absence of lipopolysaccharide. / Miyawaki, Takuya; Sogawa, Norio; Maeda, Shigeru; Kohjitani, Atsushi; Shimada, Masahiko.
In: Cytokine, Vol. 15, No. 6, 21.09.2001, p. 320-327.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Effect of midazolam on interleukin-6 mRNA expression in human peripheral blood mononuclear cells in the absence of lipopolysaccharide
AU - Miyawaki, Takuya
AU - Sogawa, Norio
AU - Maeda, Shigeru
AU - Kohjitani, Atsushi
AU - Shimada, Masahiko
PY - 2001/9/21
Y1 - 2001/9/21
N2 - Midazolam, a benzodiazepine, has an hypnotic effect via benzodiazepine receptors and is widely used as an anaesthetic. Recently, it has been suggested that benzodiazepines modulate cytokine responses. The purpose of the present study was to evaluate the effect of midazolam on interleukin-6 (IL-6) response by observing mRNA expression levels in human peripheral blood mononuclear cells (PBMCs) in the absence of lipopolysaccharide (LPS). PBMCs were isolated from healthy volunteers in endotoxin-free 0.9% sodium chloride solution. The cells were incubated for 2 h at 37°C immediately after isolation. IL-6 mRNA expression levels in the cells were quantified using reverse transcription and competitive polymerase chain reaction. It was found that midazolam time-dependently inhibited the IL-6 mRNA expression in PBMCs in the absence of LPS, and significantly inhibited the IL-6 mRNA expression at 1 μg/ml (P
AB - Midazolam, a benzodiazepine, has an hypnotic effect via benzodiazepine receptors and is widely used as an anaesthetic. Recently, it has been suggested that benzodiazepines modulate cytokine responses. The purpose of the present study was to evaluate the effect of midazolam on interleukin-6 (IL-6) response by observing mRNA expression levels in human peripheral blood mononuclear cells (PBMCs) in the absence of lipopolysaccharide (LPS). PBMCs were isolated from healthy volunteers in endotoxin-free 0.9% sodium chloride solution. The cells were incubated for 2 h at 37°C immediately after isolation. IL-6 mRNA expression levels in the cells were quantified using reverse transcription and competitive polymerase chain reaction. It was found that midazolam time-dependently inhibited the IL-6 mRNA expression in PBMCs in the absence of LPS, and significantly inhibited the IL-6 mRNA expression at 1 μg/ml (P
KW - Benzodiazepine receptors
KW - Human peripheral blood mononuclear cells
KW - Interleukin-6
KW - Midazolam
KW - mRNA
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UR - http://www.scopus.com/inward/citedby.url?scp=0035929686&partnerID=8YFLogxK
U2 - 10.1006/cyto.2001.0940
DO - 10.1006/cyto.2001.0940
M3 - Article
VL - 15
SP - 320
EP - 327
JO - Cytokine
JF - Cytokine
SN - 1043-4666
IS - 6
ER -