Effect of midazolam on interleukin-6 mRNA expression in human peripheral blood mononuclear cells in the absence of lipopolysaccharide

Takuya Miyawaki, Norio Sogawa, Shigeru Maeda, Atsushi Kohjitani, Masahiko Shimada

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Midazolam, a benzodiazepine, has an hypnotic effect via benzodiazepine receptors and is widely used as an anaesthetic. Recently, it has been suggested that benzodiazepines modulate cytokine responses. The purpose of the present study was to evaluate the effect of midazolam on interleukin-6 (IL-6) response by observing mRNA expression levels in human peripheral blood mononuclear cells (PBMCs) in the absence of lipopolysaccharide (LPS). PBMCs were isolated from healthy volunteers in endotoxin-free 0.9% sodium chloride solution. The cells were incubated for 2 h at 37°C immediately after isolation. IL-6 mRNA expression levels in the cells were quantified using reverse transcription and competitive polymerase chain reaction. It was found that midazolam time-dependently inhibited the IL-6 mRNA expression in PBMCs in the absence of LPS, and significantly inhibited the IL-6 mRNA expression at 1 μg/ml (P

Original languageEnglish
Pages (from-to)320-327
Number of pages8
JournalCytokine
Volume15
Issue number6
DOIs
Publication statusPublished - Sep 21 2001

Fingerprint

Midazolam
Lipopolysaccharides
Interleukin-6
Blood Cells
Blood
Messenger RNA
Benzodiazepines
Polymerase chain reaction
GABA-A Receptors
Transcription
Hypnotics and Sedatives
Endotoxins
Sodium Chloride
Reverse Transcription
Anesthetics
Healthy Volunteers
Cytokines
Polymerase Chain Reaction

Keywords

  • Benzodiazepine receptors
  • Human peripheral blood mononuclear cells
  • Interleukin-6
  • Midazolam
  • mRNA

ASJC Scopus subject areas

  • Endocrinology
  • Molecular Biology
  • Immunology
  • Immunology and Allergy

Cite this

Effect of midazolam on interleukin-6 mRNA expression in human peripheral blood mononuclear cells in the absence of lipopolysaccharide. / Miyawaki, Takuya; Sogawa, Norio; Maeda, Shigeru; Kohjitani, Atsushi; Shimada, Masahiko.

In: Cytokine, Vol. 15, No. 6, 21.09.2001, p. 320-327.

Research output: Contribution to journalArticle

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