Effect of Methylprednisolone on Plasma Lipid Peroxidation Induced by Lipopolysaccharide

Toshirou Kouno, Toru Egashira, Fusako Takayama, Yoshikuni Kudo, Yamanaka Yasumitsu

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The effects of methylprednisolone succinate (MP) on plasma lipid peroxidation, plasma SOD activity and superoxide production in polymorphonuclear leukocytes (PMNs) induced by lipopolysaccharide (LPS) were examined in rats in vivo and in vitro. In rats subjected to LPS treatment, plasma phosphatidylcholine hydroperoxide (PCOOH) levels significantly increased, and the plasma Cu,Zn-SOD activity decreased by about 75%. When rats were given 30 mg/kg of MP intravenously, MP suppressed the elevation of plasma PCOOH levels and partially inhibited the decrease in plasma Cu,Zn-SOD activity. MP also suppressed PMA-induced superoxide production in PMNs primed by LPS. In in vitro experiments, low concentrations of MP had no effect on NADPH-dependent lipid peroxidation, but 4 mM MP produced 50% inhibition. MP had little effect on PMA-induced superoxide production in PMNs primed by LPS. Moreover, MP had no radical-trapping effect on superoxide, hydroxyl radical and stable DPPH radical. These results suggest that the suppressive effect of plasma lipid peroxidation by MP is not due to radical-trapping effects or preventive anti-oxidation, but may involve the suppression of the lipid chain reaction in liver membrane resulting from PMA-induced superoxide anions generated by PMNs.

Original languageEnglish
Pages (from-to)163-169
Number of pages7
Journalthe japanese journal of pharmacology
Volume64
Issue number3
DOIs
Publication statusPublished - Jan 1 1994
Externally publishedYes

Keywords

  • Lipid peroxidation
  • Lipopolysaccharide
  • Methylprednisolone
  • Plasma phosphatidylcholine hydroperoxide
  • Polymorphonuclear leukocyte

ASJC Scopus subject areas

  • Pharmacology

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