Effect of methyl-β-cyclodextrin and fertilization promoting peptide on capacitation of boar spermatozoa in a protein-free medium

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Abstract

The present study was undertaken to examine the effect of methyl-β-cyclodextrin (MBCD) and fertilization promoting peptide (FPP; pGlu-Glu-ProNI-h) on the capacitation and acrosome reaction of boar spermatozoa in a protein-free medium. After washing with a protein-free medium, ejaculated spermatozoa (resuspended at 1 × 106 cells/ml) were cultured in 2 ml of modified Medium199 containing polyvinylalcohol (mM199-PVA) supplemented or not supplemented with MBCD andJ or FPP for 2 h in an atmosphere of 5% CO2 in air at 39 C. Effects of MBCD and FPP on boar spermatozoa were determined by chlortetracycline fluorescence assessment. When spermatozoa were cultured in various MBCD concentrations, capacitation was induced in a concentrationdependent manner, but spontaneous acrosome reaction was not affected. When the effect of FPP in mM199-PVA was determined, sperm capacitation was induced and spontaneous acrosome reaction was inhibited in 100 nM FPP, regardless of the absence or presence of BSA. In the last experiment, spermatozoa were cultured in mM199-PVA supplemented with various concentrations of MBCD and/or FPP. An accelerative effect of MBCD on sperm capacitation was observed in a concentrationdependent manner even in the presence of FPP, whereas 1-2 mM of MBCD neutralized the stimulatory effect of FPP on capacitation. Furthermore, FPP inhibited spontaneous acrosome reaction even in the presence of MBCD. These results demonstrate that MBCD and PFF can induce capacitation of boar spermatozoa in a protein-free medium and that FPP alone also inhibits spontaneous acrosome reaction.

Original languageEnglish
Pages (from-to)57-63
Number of pages7
JournalJournal of Reproduction and Development
Volume48
Issue number1
Publication statusPublished - 2002

Fingerprint

cyclodextrins
acrosome reaction
fertilization (reproduction)
boars
spermatozoa
peptides
sperm capacitation
proteins
chlortetracycline
washing
carbon dioxide
fluorescence
air

Keywords

  • Capacitation
  • Cholesterol efflux
  • Pig
  • Spermatozoa

ASJC Scopus subject areas

  • Animal Science and Zoology

Cite this

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title = "Effect of methyl-β-cyclodextrin and fertilization promoting peptide on capacitation of boar spermatozoa in a protein-free medium",
abstract = "The present study was undertaken to examine the effect of methyl-β-cyclodextrin (MBCD) and fertilization promoting peptide (FPP; pGlu-Glu-ProNI-h) on the capacitation and acrosome reaction of boar spermatozoa in a protein-free medium. After washing with a protein-free medium, ejaculated spermatozoa (resuspended at 1 × 106 cells/ml) were cultured in 2 ml of modified Medium199 containing polyvinylalcohol (mM199-PVA) supplemented or not supplemented with MBCD andJ or FPP for 2 h in an atmosphere of 5{\%} CO2 in air at 39 C. Effects of MBCD and FPP on boar spermatozoa were determined by chlortetracycline fluorescence assessment. When spermatozoa were cultured in various MBCD concentrations, capacitation was induced in a concentrationdependent manner, but spontaneous acrosome reaction was not affected. When the effect of FPP in mM199-PVA was determined, sperm capacitation was induced and spontaneous acrosome reaction was inhibited in 100 nM FPP, regardless of the absence or presence of BSA. In the last experiment, spermatozoa were cultured in mM199-PVA supplemented with various concentrations of MBCD and/or FPP. An accelerative effect of MBCD on sperm capacitation was observed in a concentrationdependent manner even in the presence of FPP, whereas 1-2 mM of MBCD neutralized the stimulatory effect of FPP on capacitation. Furthermore, FPP inhibited spontaneous acrosome reaction even in the presence of MBCD. These results demonstrate that MBCD and PFF can induce capacitation of boar spermatozoa in a protein-free medium and that FPP alone also inhibits spontaneous acrosome reaction.",
keywords = "Capacitation, Cholesterol efflux, Pig, Spermatozoa",
author = "Hiroaki Funahashi",
year = "2002",
language = "English",
volume = "48",
pages = "57--63",
journal = "Journal of Reproduction and Development",
issn = "0916-8818",
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T1 - Effect of methyl-β-cyclodextrin and fertilization promoting peptide on capacitation of boar spermatozoa in a protein-free medium

AU - Funahashi, Hiroaki

PY - 2002

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N2 - The present study was undertaken to examine the effect of methyl-β-cyclodextrin (MBCD) and fertilization promoting peptide (FPP; pGlu-Glu-ProNI-h) on the capacitation and acrosome reaction of boar spermatozoa in a protein-free medium. After washing with a protein-free medium, ejaculated spermatozoa (resuspended at 1 × 106 cells/ml) were cultured in 2 ml of modified Medium199 containing polyvinylalcohol (mM199-PVA) supplemented or not supplemented with MBCD andJ or FPP for 2 h in an atmosphere of 5% CO2 in air at 39 C. Effects of MBCD and FPP on boar spermatozoa were determined by chlortetracycline fluorescence assessment. When spermatozoa were cultured in various MBCD concentrations, capacitation was induced in a concentrationdependent manner, but spontaneous acrosome reaction was not affected. When the effect of FPP in mM199-PVA was determined, sperm capacitation was induced and spontaneous acrosome reaction was inhibited in 100 nM FPP, regardless of the absence or presence of BSA. In the last experiment, spermatozoa were cultured in mM199-PVA supplemented with various concentrations of MBCD and/or FPP. An accelerative effect of MBCD on sperm capacitation was observed in a concentrationdependent manner even in the presence of FPP, whereas 1-2 mM of MBCD neutralized the stimulatory effect of FPP on capacitation. Furthermore, FPP inhibited spontaneous acrosome reaction even in the presence of MBCD. These results demonstrate that MBCD and PFF can induce capacitation of boar spermatozoa in a protein-free medium and that FPP alone also inhibits spontaneous acrosome reaction.

AB - The present study was undertaken to examine the effect of methyl-β-cyclodextrin (MBCD) and fertilization promoting peptide (FPP; pGlu-Glu-ProNI-h) on the capacitation and acrosome reaction of boar spermatozoa in a protein-free medium. After washing with a protein-free medium, ejaculated spermatozoa (resuspended at 1 × 106 cells/ml) were cultured in 2 ml of modified Medium199 containing polyvinylalcohol (mM199-PVA) supplemented or not supplemented with MBCD andJ or FPP for 2 h in an atmosphere of 5% CO2 in air at 39 C. Effects of MBCD and FPP on boar spermatozoa were determined by chlortetracycline fluorescence assessment. When spermatozoa were cultured in various MBCD concentrations, capacitation was induced in a concentrationdependent manner, but spontaneous acrosome reaction was not affected. When the effect of FPP in mM199-PVA was determined, sperm capacitation was induced and spontaneous acrosome reaction was inhibited in 100 nM FPP, regardless of the absence or presence of BSA. In the last experiment, spermatozoa were cultured in mM199-PVA supplemented with various concentrations of MBCD and/or FPP. An accelerative effect of MBCD on sperm capacitation was observed in a concentrationdependent manner even in the presence of FPP, whereas 1-2 mM of MBCD neutralized the stimulatory effect of FPP on capacitation. Furthermore, FPP inhibited spontaneous acrosome reaction even in the presence of MBCD. These results demonstrate that MBCD and PFF can induce capacitation of boar spermatozoa in a protein-free medium and that FPP alone also inhibits spontaneous acrosome reaction.

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