Effect of matrix metalloproteinase on autolysis of sea cucumber Stichopus japonicus

Li Ming Sun; Ting Ting Wang; Bei Wei Zhu; Hai Ling Niu; Rui Zhang; Hong Man Hou; Gong Liang Zhang; Yoshiyuki Murata

doi:10.1007/s10068-013-0210-x

Effect of matrix metalloproteinase on autolysis of sea cucumber Stichopus japonicus

Li Ming Sun, Ting Ting Wang, Bei Wei Zhu, Hai Ling Niu, Rui Zhang, Hong Man Hou, Gong Liang Zhang, Yoshiyuki Murata

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

To investigate the relationship between matrix metalloproteinase (MMPs) and autolysis of sea cucumber Stichopus japonicus, the dermis homogenate was incubated at 25°C to induce autolysis. EDTA Na₂ and 1,10-phenanthroline were used to verify the effect of MMPs on autolysis, which was measured by soluble protein and protein pattern. Soluble protein level increased during a 6-h autolysis process. SDS-PAGE demonstrated obvious protein degradation with the concomitant occurrence of degradation products. The above two indicators could be inhibited significantly by EDTA Na₂ and 1,10-phenanthroline, indicating that MMPs might play a significant role in autolysis of sea cucumber.

Original language	English
Pages (from-to)	1-3
Number of pages	3
Journal	Food Science and Biotechnology
Volume	22
Issue number	5
DOIs	https://doi.org/10.1007/s10068-013-0210-x
Publication status	Published - Oct 2013

Keywords

autolysis
matrix metalloproteinase
sea cucumber (Stichopus japonicus)

ASJC Scopus subject areas

Biotechnology
Food Science
Applied Microbiology and Biotechnology

Access to Document

10.1007/s10068-013-0210-x

Cite this

@article{d8fea615456d4db782141478e964526f,

title = "Effect of matrix metalloproteinase on autolysis of sea cucumber Stichopus japonicus",

abstract = "To investigate the relationship between matrix metalloproteinase (MMPs) and autolysis of sea cucumber Stichopus japonicus, the dermis homogenate was incubated at 25°C to induce autolysis. EDTA Na2 and 1,10-phenanthroline were used to verify the effect of MMPs on autolysis, which was measured by soluble protein and protein pattern. Soluble protein level increased during a 6-h autolysis process. SDS-PAGE demonstrated obvious protein degradation with the concomitant occurrence of degradation products. The above two indicators could be inhibited significantly by EDTA Na2 and 1,10-phenanthroline, indicating that MMPs might play a significant role in autolysis of sea cucumber.",

keywords = "autolysis, matrix metalloproteinase, sea cucumber (Stichopus japonicus)",

author = "Sun, {Li Ming} and Wang, {Ting Ting} and Zhu, {Bei Wei} and Niu, {Hai Ling} and Rui Zhang and Hou, {Hong Man} and Zhang, {Gong Liang} and Yoshiyuki Murata",

note = "Funding Information: Acknowledgments This work was supported financially by “The National Natural Science Foundation of China (No. 31201300)” and “Liaoning Provincial Committee of Education (LJQ2012048)”.",

year = "2013",

month = oct,

doi = "10.1007/s10068-013-0210-x",

language = "English",

volume = "22",

pages = "1--3",

journal = "Food Science and Biotechnology",

issn = "1226-7708",

publisher = "Springer Netherlands",

number = "5",

}

TY - JOUR

T1 - Effect of matrix metalloproteinase on autolysis of sea cucumber Stichopus japonicus

AU - Sun, Li Ming

AU - Wang, Ting Ting

AU - Zhu, Bei Wei

AU - Niu, Hai Ling

AU - Zhang, Rui

AU - Hou, Hong Man

AU - Zhang, Gong Liang

AU - Murata, Yoshiyuki

N1 - Funding Information: Acknowledgments This work was supported financially by “The National Natural Science Foundation of China (No. 31201300)” and “Liaoning Provincial Committee of Education (LJQ2012048)”.

PY - 2013/10

Y1 - 2013/10

N2 - To investigate the relationship between matrix metalloproteinase (MMPs) and autolysis of sea cucumber Stichopus japonicus, the dermis homogenate was incubated at 25°C to induce autolysis. EDTA Na2 and 1,10-phenanthroline were used to verify the effect of MMPs on autolysis, which was measured by soluble protein and protein pattern. Soluble protein level increased during a 6-h autolysis process. SDS-PAGE demonstrated obvious protein degradation with the concomitant occurrence of degradation products. The above two indicators could be inhibited significantly by EDTA Na2 and 1,10-phenanthroline, indicating that MMPs might play a significant role in autolysis of sea cucumber.

AB - To investigate the relationship between matrix metalloproteinase (MMPs) and autolysis of sea cucumber Stichopus japonicus, the dermis homogenate was incubated at 25°C to induce autolysis. EDTA Na2 and 1,10-phenanthroline were used to verify the effect of MMPs on autolysis, which was measured by soluble protein and protein pattern. Soluble protein level increased during a 6-h autolysis process. SDS-PAGE demonstrated obvious protein degradation with the concomitant occurrence of degradation products. The above two indicators could be inhibited significantly by EDTA Na2 and 1,10-phenanthroline, indicating that MMPs might play a significant role in autolysis of sea cucumber.

KW - autolysis

KW - matrix metalloproteinase

KW - sea cucumber (Stichopus japonicus)

UR - http://www.scopus.com/inward/record.url?scp=84887522669&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84887522669&partnerID=8YFLogxK

U2 - 10.1007/s10068-013-0210-x

DO - 10.1007/s10068-013-0210-x

M3 - Article

AN - SCOPUS:84887522669

SN - 1226-7708

VL - 22

SP - 1

EP - 3

JO - Food Science and Biotechnology

JF - Food Science and Biotechnology

IS - 5

ER -

Effect of matrix metalloproteinase on autolysis of sea cucumber Stichopus japonicus

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this