Effect of matrix metalloproteinase on autolysis of sea cucumber Stichopus japonicus

Li Ming Sun, Ting Ting Wang, Bei Wei Zhu, Hai Ling Niu, Rui Zhang, Hong Man Hou, Gong Liang Zhang, Yoshiyuki Murata

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

To investigate the relationship between matrix metalloproteinase (MMPs) and autolysis of sea cucumber Stichopus japonicus, the dermis homogenate was incubated at 25°C to induce autolysis. EDTA Na2 and 1,10-phenanthroline were used to verify the effect of MMPs on autolysis, which was measured by soluble protein and protein pattern. Soluble protein level increased during a 6-h autolysis process. SDS-PAGE demonstrated obvious protein degradation with the concomitant occurrence of degradation products. The above two indicators could be inhibited significantly by EDTA Na2 and 1,10-phenanthroline, indicating that MMPs might play a significant role in autolysis of sea cucumber.

Original languageEnglish
Pages (from-to)1-3
Number of pages3
JournalFood Science and Biotechnology
Volume22
Issue number5
DOIs
Publication statusPublished - Oct 2013

Fingerprint

Stichopus
Sea Cucumbers
Apostichopus japonicus
Autolysis
autolysis
Holothuroidea
metalloproteinases
Matrix Metalloproteinases
Edetic Acid
Proteins
proteins
dermis
Dermis
protein degradation
Proteolysis
Polyacrylamide Gel Electrophoresis
degradation

Keywords

  • autolysis
  • matrix metalloproteinase
  • sea cucumber (Stichopus japonicus)

ASJC Scopus subject areas

  • Food Science
  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

Effect of matrix metalloproteinase on autolysis of sea cucumber Stichopus japonicus. / Sun, Li Ming; Wang, Ting Ting; Zhu, Bei Wei; Niu, Hai Ling; Zhang, Rui; Hou, Hong Man; Zhang, Gong Liang; Murata, Yoshiyuki.

In: Food Science and Biotechnology, Vol. 22, No. 5, 10.2013, p. 1-3.

Research output: Contribution to journalArticle

Sun, Li Ming ; Wang, Ting Ting ; Zhu, Bei Wei ; Niu, Hai Ling ; Zhang, Rui ; Hou, Hong Man ; Zhang, Gong Liang ; Murata, Yoshiyuki. / Effect of matrix metalloproteinase on autolysis of sea cucumber Stichopus japonicus. In: Food Science and Biotechnology. 2013 ; Vol. 22, No. 5. pp. 1-3.
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AU - Hou, Hong Man

AU - Zhang, Gong Liang

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