Effect of maternal age on mitochondrial DNA copy number, ATP content and IVF outcome of bovine oocytes

Hisataka Iwata, Hiroya Goto, Hiroshi Tanaka, Yosuke Sakaguchi, Koji Kimura, Takehito Kuwayama, Yashunori Monji

Research output: Contribution to journalArticle

77 Citations (Scopus)

Abstract

The primary aim of the present study was to examine the effect of maternal age (in months) on mitochondrial DNA copy number (Mt number), ATP content and IVF outcome of bovine oocytes. We also compared the Mt number of oocytes with fertilisation outcome and ATP content. Oocytes were collected from cows aged 20-204 months and the Mt number was determined by real-time polymerase chain reaction. The Mt number in immature and mature oocytes was determined to be 368118 and 807794, respectively; the ATP content in these oocytes was 1.2 and 2.0pM, respectively. Both Mt number and ATP content increased during oocyte maturation. However, after 90 months of age, the Mt number of mature oocytes decreased with increasing maternal age, whereas the ATP content of mature oocytes was positively correlated with maternal age (P89 months) cows. Thus, we conclude that Mt number, the ATP content and fertilisation outcome of bovine oocytes are affected by maternal age.

Original languageEnglish
Pages (from-to)424-432
Number of pages9
JournalReproduction, Fertility and Development
Volume23
Issue number3
DOIs
Publication statusPublished - 2011
Externally publishedYes

Fingerprint

Maternal Age
maternal effect
Mitochondrial DNA
Oocytes
oocytes
mitochondrial DNA
Adenosine Triphosphate
cattle
Fertilization
cows
Real-Time Polymerase Chain Reaction
quantitative polymerase chain reaction
immatures

Keywords

  • cow

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology
  • Genetics
  • Endocrinology
  • Developmental Biology
  • Reproductive Medicine
  • Animal Science and Zoology

Cite this

Effect of maternal age on mitochondrial DNA copy number, ATP content and IVF outcome of bovine oocytes. / Iwata, Hisataka; Goto, Hiroya; Tanaka, Hiroshi; Sakaguchi, Yosuke; Kimura, Koji; Kuwayama, Takehito; Monji, Yashunori.

In: Reproduction, Fertility and Development, Vol. 23, No. 3, 2011, p. 424-432.

Research output: Contribution to journalArticle

Iwata, Hisataka ; Goto, Hiroya ; Tanaka, Hiroshi ; Sakaguchi, Yosuke ; Kimura, Koji ; Kuwayama, Takehito ; Monji, Yashunori. / Effect of maternal age on mitochondrial DNA copy number, ATP content and IVF outcome of bovine oocytes. In: Reproduction, Fertility and Development. 2011 ; Vol. 23, No. 3. pp. 424-432.
@article{15bd9dd64c914e1f9ad6b9185e6fd654,
title = "Effect of maternal age on mitochondrial DNA copy number, ATP content and IVF outcome of bovine oocytes",
abstract = "The primary aim of the present study was to examine the effect of maternal age (in months) on mitochondrial DNA copy number (Mt number), ATP content and IVF outcome of bovine oocytes. We also compared the Mt number of oocytes with fertilisation outcome and ATP content. Oocytes were collected from cows aged 20-204 months and the Mt number was determined by real-time polymerase chain reaction. The Mt number in immature and mature oocytes was determined to be 368118 and 807794, respectively; the ATP content in these oocytes was 1.2 and 2.0pM, respectively. Both Mt number and ATP content increased during oocyte maturation. However, after 90 months of age, the Mt number of mature oocytes decreased with increasing maternal age, whereas the ATP content of mature oocytes was positively correlated with maternal age (P89 months) cows. Thus, we conclude that Mt number, the ATP content and fertilisation outcome of bovine oocytes are affected by maternal age.",
keywords = "cow",
author = "Hisataka Iwata and Hiroya Goto and Hiroshi Tanaka and Yosuke Sakaguchi and Koji Kimura and Takehito Kuwayama and Yashunori Monji",
year = "2011",
doi = "10.1071/RD10133",
language = "English",
volume = "23",
pages = "424--432",
journal = "Reproduction, Fertility and Development",
issn = "1031-3613",
publisher = "CSIRO",
number = "3",

}

TY - JOUR

T1 - Effect of maternal age on mitochondrial DNA copy number, ATP content and IVF outcome of bovine oocytes

AU - Iwata, Hisataka

AU - Goto, Hiroya

AU - Tanaka, Hiroshi

AU - Sakaguchi, Yosuke

AU - Kimura, Koji

AU - Kuwayama, Takehito

AU - Monji, Yashunori

PY - 2011

Y1 - 2011

N2 - The primary aim of the present study was to examine the effect of maternal age (in months) on mitochondrial DNA copy number (Mt number), ATP content and IVF outcome of bovine oocytes. We also compared the Mt number of oocytes with fertilisation outcome and ATP content. Oocytes were collected from cows aged 20-204 months and the Mt number was determined by real-time polymerase chain reaction. The Mt number in immature and mature oocytes was determined to be 368118 and 807794, respectively; the ATP content in these oocytes was 1.2 and 2.0pM, respectively. Both Mt number and ATP content increased during oocyte maturation. However, after 90 months of age, the Mt number of mature oocytes decreased with increasing maternal age, whereas the ATP content of mature oocytes was positively correlated with maternal age (P89 months) cows. Thus, we conclude that Mt number, the ATP content and fertilisation outcome of bovine oocytes are affected by maternal age.

AB - The primary aim of the present study was to examine the effect of maternal age (in months) on mitochondrial DNA copy number (Mt number), ATP content and IVF outcome of bovine oocytes. We also compared the Mt number of oocytes with fertilisation outcome and ATP content. Oocytes were collected from cows aged 20-204 months and the Mt number was determined by real-time polymerase chain reaction. The Mt number in immature and mature oocytes was determined to be 368118 and 807794, respectively; the ATP content in these oocytes was 1.2 and 2.0pM, respectively. Both Mt number and ATP content increased during oocyte maturation. However, after 90 months of age, the Mt number of mature oocytes decreased with increasing maternal age, whereas the ATP content of mature oocytes was positively correlated with maternal age (P89 months) cows. Thus, we conclude that Mt number, the ATP content and fertilisation outcome of bovine oocytes are affected by maternal age.

KW - cow

UR - http://www.scopus.com/inward/record.url?scp=79957566613&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79957566613&partnerID=8YFLogxK

U2 - 10.1071/RD10133

DO - 10.1071/RD10133

M3 - Article

C2 - 21426860

AN - SCOPUS:79957566613

VL - 23

SP - 424

EP - 432

JO - Reproduction, Fertility and Development

JF - Reproduction, Fertility and Development

SN - 1031-3613

IS - 3

ER -