Effect of intranasal corticosteroid on pre-onset activation of eosinophils and mast cells in experimental Japanese cedar pollinosis

Yasuyuki Noyama, Mitsuhiro Okano, Tazuko Fujiwara, Shin Kariya, Sei ichiro Makihara, Takenori Haruna, Kengo Kanai, Takaya Higaki, Kazunori Nishizaki

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Background Minimal persistent inflammation (MPI) contributes to hyperreactivity in allergic rhinitis. However, little is known regarding whether pre-onset activation of eosinophils and mast cells is present or not in Japanese cedar pollinosis (JCP). Furthermore, a prophylactic effect of intranasal corticosteroids on such MPI in JCP has not been investigated. Methods We designed a double-blinded, randomized, placebo-controlled, crossover trial. Twenty patients with JCP were examined outside the pollen season (UMIN000008410). Nasal provocation with paper discs containing extracts of Japanese cedar pollen was performed once a day for 3 consecutive days. Onset of nasal symptoms was monitored over 15 min after each provocation. The levels of eosinophil cationic protein (ECP) and tryptase in nasal secretions were examined. Fluticasone furoate nasal spray or placebo treatment was started one day before the first provocation. Results In the placebo group, 25% of the patients showed onset of nasal symptoms following provocation on the first day. In addition, 75% and 68% of the patients showed symptom onset on the second and third day of provocation, respectively. After the first provocation, the levels of ECP and tryptase in nasal secretions were significantly increased. These increases were seen not only in symptomatic but also in asymptomatic subjects in response to provocation, and the levels were similar between these subjects. Prophylactic treatment with fluticasone significantly suppressed the increase in nasal ECP and tryptase associated with repeated provocations. Conclusions These results suggest that pre-onset activation of eosinophils and mast cells is present in experimental JCP, and that prophylactic treatment with intranasal corticosteroids has the potential to control such activation.

Original languageEnglish
Pages (from-to)259-265
Number of pages7
JournalAllergology International
Volume65
Issue number3
DOIs
Publication statusPublished - Jul 1 2016

Fingerprint

Cryptomeria
Seasonal Allergic Rhinitis
Nose
Eosinophils
Mast Cells
Adrenal Cortex Hormones
Eosinophil Cationic Protein
Tryptases
Placebos
Pollen
Inflammation
Nasal Sprays
Cross-Over Studies
Therapeutics

Keywords

  • Eosinophil cationic protein
  • Intranasal corticosteroids
  • Japanese cedar pollinosis
  • Minimal persistent inflammation
  • Tryptase

ASJC Scopus subject areas

  • Immunology and Allergy

Cite this

Effect of intranasal corticosteroid on pre-onset activation of eosinophils and mast cells in experimental Japanese cedar pollinosis. / Noyama, Yasuyuki; Okano, Mitsuhiro; Fujiwara, Tazuko; Kariya, Shin; Makihara, Sei ichiro; Haruna, Takenori; Kanai, Kengo; Higaki, Takaya; Nishizaki, Kazunori.

In: Allergology International, Vol. 65, No. 3, 01.07.2016, p. 259-265.

Research output: Contribution to journalArticle

Noyama, Yasuyuki ; Okano, Mitsuhiro ; Fujiwara, Tazuko ; Kariya, Shin ; Makihara, Sei ichiro ; Haruna, Takenori ; Kanai, Kengo ; Higaki, Takaya ; Nishizaki, Kazunori. / Effect of intranasal corticosteroid on pre-onset activation of eosinophils and mast cells in experimental Japanese cedar pollinosis. In: Allergology International. 2016 ; Vol. 65, No. 3. pp. 259-265.
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T1 - Effect of intranasal corticosteroid on pre-onset activation of eosinophils and mast cells in experimental Japanese cedar pollinosis

AU - Noyama, Yasuyuki

AU - Okano, Mitsuhiro

AU - Fujiwara, Tazuko

AU - Kariya, Shin

AU - Makihara, Sei ichiro

AU - Haruna, Takenori

AU - Kanai, Kengo

AU - Higaki, Takaya

AU - Nishizaki, Kazunori

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N2 - Background Minimal persistent inflammation (MPI) contributes to hyperreactivity in allergic rhinitis. However, little is known regarding whether pre-onset activation of eosinophils and mast cells is present or not in Japanese cedar pollinosis (JCP). Furthermore, a prophylactic effect of intranasal corticosteroids on such MPI in JCP has not been investigated. Methods We designed a double-blinded, randomized, placebo-controlled, crossover trial. Twenty patients with JCP were examined outside the pollen season (UMIN000008410). Nasal provocation with paper discs containing extracts of Japanese cedar pollen was performed once a day for 3 consecutive days. Onset of nasal symptoms was monitored over 15 min after each provocation. The levels of eosinophil cationic protein (ECP) and tryptase in nasal secretions were examined. Fluticasone furoate nasal spray or placebo treatment was started one day before the first provocation. Results In the placebo group, 25% of the patients showed onset of nasal symptoms following provocation on the first day. In addition, 75% and 68% of the patients showed symptom onset on the second and third day of provocation, respectively. After the first provocation, the levels of ECP and tryptase in nasal secretions were significantly increased. These increases were seen not only in symptomatic but also in asymptomatic subjects in response to provocation, and the levels were similar between these subjects. Prophylactic treatment with fluticasone significantly suppressed the increase in nasal ECP and tryptase associated with repeated provocations. Conclusions These results suggest that pre-onset activation of eosinophils and mast cells is present in experimental JCP, and that prophylactic treatment with intranasal corticosteroids has the potential to control such activation.

AB - Background Minimal persistent inflammation (MPI) contributes to hyperreactivity in allergic rhinitis. However, little is known regarding whether pre-onset activation of eosinophils and mast cells is present or not in Japanese cedar pollinosis (JCP). Furthermore, a prophylactic effect of intranasal corticosteroids on such MPI in JCP has not been investigated. Methods We designed a double-blinded, randomized, placebo-controlled, crossover trial. Twenty patients with JCP were examined outside the pollen season (UMIN000008410). Nasal provocation with paper discs containing extracts of Japanese cedar pollen was performed once a day for 3 consecutive days. Onset of nasal symptoms was monitored over 15 min after each provocation. The levels of eosinophil cationic protein (ECP) and tryptase in nasal secretions were examined. Fluticasone furoate nasal spray or placebo treatment was started one day before the first provocation. Results In the placebo group, 25% of the patients showed onset of nasal symptoms following provocation on the first day. In addition, 75% and 68% of the patients showed symptom onset on the second and third day of provocation, respectively. After the first provocation, the levels of ECP and tryptase in nasal secretions were significantly increased. These increases were seen not only in symptomatic but also in asymptomatic subjects in response to provocation, and the levels were similar between these subjects. Prophylactic treatment with fluticasone significantly suppressed the increase in nasal ECP and tryptase associated with repeated provocations. Conclusions These results suggest that pre-onset activation of eosinophils and mast cells is present in experimental JCP, and that prophylactic treatment with intranasal corticosteroids has the potential to control such activation.

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KW - Minimal persistent inflammation

KW - Tryptase

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