TY - JOUR
T1 - Effect of interfacial serum proteins on melanoma cell adhesion to biodegradable poly(l-lactic acid) microspheres coated with hydroxyapatite
AU - Shinto, Hiroyuki
AU - Hirata, Takuya
AU - Fukasawa, Tomonori
AU - Fujii, Syuji
AU - Maeda, Hayata
AU - Okada, Masahiro
AU - Nakamura, Yoshinobu
AU - Furuzono, Tsutomu
N1 - Funding Information:
H.S. thanks for financial support by the Ministry of Education, Culture, Sports, Science and Technology (MEXT) in Japan (Grants-in-Aid for Scientific Research, no. 22686072 / 23656489 ; Strategic Development of Research Infrastructure for Private Universities , no. S1201010 ), the Japan Society for the Promotion of Science (JSPS; Core-to-Core Program, no. 18004 ), and the Information Center of Particle Technology, Japan .
PY - 2013/8/1
Y1 - 2013/8/1
N2 - We have measured the interaction forces between a murine melanoma cell and a poly(l-lactic acid) (PLLA) microsphere coated with/without hydroxyapatite (HAp) nanoparticles (i.e., an HAp/PLLA or a bare PLLA microsphere) in a serum-free culture medium, using atomic force microscopy (AFM) with colloid probe technique, in order to investigate how the HAp-nanoparticle coating as well as interfacial serum proteins influence the cell-microsphere adhesion. The cell adhesion force of the HAp/PLLA microspheres was 1.4-fold stronger than that of the bare PLLA microspheres. When the microspheres were pretreated with a culture medium supplemented with 10% fetal bovine serum, the cell adhesion force of the HAp/PLLA microspheres was increased by a factor of 2.1; in contrast, no change was observed in the cell adhesion force of the bare PLLA microspheres before/after the pretreatment. Indeed, the cell adhesion force of the HAp/PLLA was 2.8-fold larger than that of the bare PLLA after the pretreatment. Additionally, we have investigated the effect of interfacial serum proteins on the zeta potentials of these microspheres. On the basis of the obtained results, possible mechanism of cell adhesion to the HAp/PLLA and bare PLLA microspheres in the presence/absence of the interfacial serum proteins is discussed.
AB - We have measured the interaction forces between a murine melanoma cell and a poly(l-lactic acid) (PLLA) microsphere coated with/without hydroxyapatite (HAp) nanoparticles (i.e., an HAp/PLLA or a bare PLLA microsphere) in a serum-free culture medium, using atomic force microscopy (AFM) with colloid probe technique, in order to investigate how the HAp-nanoparticle coating as well as interfacial serum proteins influence the cell-microsphere adhesion. The cell adhesion force of the HAp/PLLA microspheres was 1.4-fold stronger than that of the bare PLLA microspheres. When the microspheres were pretreated with a culture medium supplemented with 10% fetal bovine serum, the cell adhesion force of the HAp/PLLA microspheres was increased by a factor of 2.1; in contrast, no change was observed in the cell adhesion force of the bare PLLA microspheres before/after the pretreatment. Indeed, the cell adhesion force of the HAp/PLLA was 2.8-fold larger than that of the bare PLLA after the pretreatment. Additionally, we have investigated the effect of interfacial serum proteins on the zeta potentials of these microspheres. On the basis of the obtained results, possible mechanism of cell adhesion to the HAp/PLLA and bare PLLA microspheres in the presence/absence of the interfacial serum proteins is discussed.
KW - Atomic force microscopy
KW - Cell adhesion
KW - Core-shell microspheres
KW - Hydroxyapatite
KW - Poly(l-lactic acid)
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U2 - 10.1016/j.colsurfb.2013.02.021
DO - 10.1016/j.colsurfb.2013.02.021
M3 - Article
C2 - 23524077
AN - SCOPUS:84875585629
VL - 108
SP - 8
EP - 15
JO - Colloids and Surfaces B: Biointerfaces
JF - Colloids and Surfaces B: Biointerfaces
SN - 0927-7765
ER -