TY - JOUR
T1 - Effect of insect pollinator on inbreeding versus outbreeding in open pollinated strawberry seeds
AU - Yanagi, Tomohiro
AU - Miura, Hiroto
AU - Isobe, Sachiko
AU - Okuda, Nobuyuki
AU - Yoshida, Yuichi
N1 - Publisher Copyright:
© 2016 Elsevier B.V.
Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 2017/1/27
Y1 - 2017/1/27
N2 - In Japan, honey bees, bumble bees, and flies are used in strawberry production to produce normally shaped fruits. These insects may determine the inbreeding or outbreeding of strawberry flowers. Whole-genome DNA of the strawberry cultivar ‘Shizutakara’ showed no amplification of DNA fragments when PCR was performed with a F3H CAPS DNA marker. However, in other ordinary strawberry cultivars, amplification of DNA fragments was observed on PCR with the F3H. Thus, growing one plant of ‘Shizutakara’ with other cultivars and a single insect species, harvesting ‘Shizutakara’ fruit, and performing DNA analysis of the seeds could show whether the seed resulted from inbreeding or outbreeding, as the DNA fragments would be amplified from outbred but not from inbred seed. On this basis, differences in pollination mode among these three pollinators were analyzed. DNA analysis showed that DNA fragments could not be detected in seeds obtained by fly pollination in the greenhouse. Flies appeared to promote inbreeding. For honey bees and bumble bee, amplified DNA fragments could be detected. Some seeds pollinated by honey bees and bumble bee appeared to be of outbred origin. From these results, differences in pollination mode among insect pollinators were identified.
AB - In Japan, honey bees, bumble bees, and flies are used in strawberry production to produce normally shaped fruits. These insects may determine the inbreeding or outbreeding of strawberry flowers. Whole-genome DNA of the strawberry cultivar ‘Shizutakara’ showed no amplification of DNA fragments when PCR was performed with a F3H CAPS DNA marker. However, in other ordinary strawberry cultivars, amplification of DNA fragments was observed on PCR with the F3H. Thus, growing one plant of ‘Shizutakara’ with other cultivars and a single insect species, harvesting ‘Shizutakara’ fruit, and performing DNA analysis of the seeds could show whether the seed resulted from inbreeding or outbreeding, as the DNA fragments would be amplified from outbred but not from inbred seed. On this basis, differences in pollination mode among these three pollinators were analyzed. DNA analysis showed that DNA fragments could not be detected in seeds obtained by fly pollination in the greenhouse. Flies appeared to promote inbreeding. For honey bees and bumble bee, amplified DNA fragments could be detected. Some seeds pollinated by honey bees and bumble bee appeared to be of outbred origin. From these results, differences in pollination mode among insect pollinators were identified.
KW - Bumble bee
KW - CAPS marker
KW - DNA analysis
KW - Fly
KW - Fragaria × ananassa
KW - Honey bee
KW - Inbreeding
KW - Outbreeding
KW - Strawberry
UR - http://www.scopus.com/inward/record.url?scp=85007270732&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85007270732&partnerID=8YFLogxK
U2 - 10.1016/j.scienta.2016.12.015
DO - 10.1016/j.scienta.2016.12.015
M3 - Article
AN - SCOPUS:85007270732
VL - 215
SP - 112
EP - 116
JO - Scientia Horticulturae
JF - Scientia Horticulturae
SN - 0304-4238
ER -