Effect of high temperature on grain filling period, yield, amylose content and activity of starch biosynthesis enzymes in endosperm of basmati rice

Nisar Ahmed, Ian J. Tetlow, Sehar Nawaz, Ahsan Iqbal, Muhammad Mubin, Muhammad Shah Nawaz ul Rehman, Aisha Butt, David A. Lightfoot, Masahiko Maekawa

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

BACKGROUND: High temperature during grain filling affects yield, starch amylose content and activity of starch biosynthesis enzymes in basmati rice. To investigate the physiological mechanisms underpinning the effects of high temperature on rice grain, basmati rice was grown under two temperature conditions - 32 and 22 °C - during grain filling. RESULTS: High temperature decreased the grain filling period from 32 to 26 days, reducing yield by 6%, and caused a reduction in total starch (3.1%) and amylose content (22%). Measurable activities of key enzymes involved in sucrose to starch conversion, sucrose synthase, ADP-glucose pyrophosphorylase, starch phosphorylase and soluble starch synthase in endosperms developed at 32 °C were lower than those at 22 °C compared with similar ripening stage on an endosperm basis. In particular, granule-bound starch synthase (GBSS) activity was significantly lower than corresponding activity in endosperms developing at 22 °C during all developmental stages analyzed. CONCLUSION: Results suggest changes in amylose/amylopectin ratio observed in plants grown at 32 °C was attributable to a reduction in activity of GBSS, the sole enzyme responsible for amylose biosynthesis.

Original languageEnglish
Pages (from-to)2237-2243
Number of pages7
JournalJournal of the Science of Food and Agriculture
Volume95
Issue number11
DOIs
Publication statusPublished - Aug 30 2015

Fingerprint

Amylose
Endosperm
Starch Synthase
filling period
amylose
Starch
endosperm
starch synthase
biosynthesis
starch
rice
Temperature
Enzymes
enzymes
Starch Phosphorylase
temperature
Glucose-1-Phosphate Adenylyltransferase
granules
Amylopectin
sucrose synthase

Keywords

  • Amylose content
  • Basmati rice
  • High temperature
  • Starch biosynthesis

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Food Science
  • Nutrition and Dietetics
  • Biotechnology

Cite this

Effect of high temperature on grain filling period, yield, amylose content and activity of starch biosynthesis enzymes in endosperm of basmati rice. / Ahmed, Nisar; Tetlow, Ian J.; Nawaz, Sehar; Iqbal, Ahsan; Mubin, Muhammad; Nawaz ul Rehman, Muhammad Shah; Butt, Aisha; Lightfoot, David A.; Maekawa, Masahiko.

In: Journal of the Science of Food and Agriculture, Vol. 95, No. 11, 30.08.2015, p. 2237-2243.

Research output: Contribution to journalArticle

Ahmed, N, Tetlow, IJ, Nawaz, S, Iqbal, A, Mubin, M, Nawaz ul Rehman, MS, Butt, A, Lightfoot, DA & Maekawa, M 2015, 'Effect of high temperature on grain filling period, yield, amylose content and activity of starch biosynthesis enzymes in endosperm of basmati rice', Journal of the Science of Food and Agriculture, vol. 95, no. 11, pp. 2237-2243. https://doi.org/10.1002/jsfa.6941
Ahmed, Nisar ; Tetlow, Ian J. ; Nawaz, Sehar ; Iqbal, Ahsan ; Mubin, Muhammad ; Nawaz ul Rehman, Muhammad Shah ; Butt, Aisha ; Lightfoot, David A. ; Maekawa, Masahiko. / Effect of high temperature on grain filling period, yield, amylose content and activity of starch biosynthesis enzymes in endosperm of basmati rice. In: Journal of the Science of Food and Agriculture. 2015 ; Vol. 95, No. 11. pp. 2237-2243.
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AU - Iqbal, Ahsan

AU - Mubin, Muhammad

AU - Nawaz ul Rehman, Muhammad Shah

AU - Butt, Aisha

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N2 - BACKGROUND: High temperature during grain filling affects yield, starch amylose content and activity of starch biosynthesis enzymes in basmati rice. To investigate the physiological mechanisms underpinning the effects of high temperature on rice grain, basmati rice was grown under two temperature conditions - 32 and 22 °C - during grain filling. RESULTS: High temperature decreased the grain filling period from 32 to 26 days, reducing yield by 6%, and caused a reduction in total starch (3.1%) and amylose content (22%). Measurable activities of key enzymes involved in sucrose to starch conversion, sucrose synthase, ADP-glucose pyrophosphorylase, starch phosphorylase and soluble starch synthase in endosperms developed at 32 °C were lower than those at 22 °C compared with similar ripening stage on an endosperm basis. In particular, granule-bound starch synthase (GBSS) activity was significantly lower than corresponding activity in endosperms developing at 22 °C during all developmental stages analyzed. CONCLUSION: Results suggest changes in amylose/amylopectin ratio observed in plants grown at 32 °C was attributable to a reduction in activity of GBSS, the sole enzyme responsible for amylose biosynthesis.

AB - BACKGROUND: High temperature during grain filling affects yield, starch amylose content and activity of starch biosynthesis enzymes in basmati rice. To investigate the physiological mechanisms underpinning the effects of high temperature on rice grain, basmati rice was grown under two temperature conditions - 32 and 22 °C - during grain filling. RESULTS: High temperature decreased the grain filling period from 32 to 26 days, reducing yield by 6%, and caused a reduction in total starch (3.1%) and amylose content (22%). Measurable activities of key enzymes involved in sucrose to starch conversion, sucrose synthase, ADP-glucose pyrophosphorylase, starch phosphorylase and soluble starch synthase in endosperms developed at 32 °C were lower than those at 22 °C compared with similar ripening stage on an endosperm basis. In particular, granule-bound starch synthase (GBSS) activity was significantly lower than corresponding activity in endosperms developing at 22 °C during all developmental stages analyzed. CONCLUSION: Results suggest changes in amylose/amylopectin ratio observed in plants grown at 32 °C was attributable to a reduction in activity of GBSS, the sole enzyme responsible for amylose biosynthesis.

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