Effect of heat stress on serotonin-2A receptor-mediated intracellular calcium mobilization in rat C6 glioma cells

A. Kagaya, A. Okada, H. Jitsuiki, Y. Tawara, M. Inagaki, M. Takebayashi, T. Saeki, A. Nishida, Y. Nakata, S. Yamawaki

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

This study was conducted to investigate an effect of heat stress at 44 °C for 30min on intracellular Ca 2+ signaling system and on heat shock protein (HSP)-70 expression. 5-HT-induced Ca 2+ mobilization was reduced 1, 3 and 6hrs after heat stress, and recovered to the control level 12 and 24hrs after heat stress. One hr after heat stress, Ca 2+ rise was significantly decreased when the cells were stimulated by any concentration of 5-HT. Thrombin-induced Ca 2+ increase was also markedly reduced 1hr after heat stress. HSP-70 level was increased 6 and 9 hr after heat stress. In HSP synthesis inhibitor quercetin-treated cells, HSP-70 expression was not enhanced after heat stress, and Ca 2+ rise in response to 5-HT did not return to the control level. However, the Ca 2+ rise induced by 5-HT was not restored to the control level after stress in Ac-Asp-Glu-Val-Asp-H (DEVD)-exposed cells while DEVD had little effect on heat stress-induced synthesis of HSP-70. Dexamethasone did not alter the change in HSP-70 expression or Ca 2+ response after heat stress. These results indicate that heat stress attenuated 5-HT-induced Ca 2+ mobilization and that HSP-70 expression played an important role in recovery from Ca 2+ impairment, possibly via protease activity in C6 cells.

Original languageEnglish
Pages (from-to)919-929
Number of pages11
JournalJournal of Neural Transmission
Volume107
Issue number8-9
DOIs
Publication statusPublished - 2000
Externally publishedYes

Fingerprint

Receptor, Serotonin, 5-HT2A
Glioma
HSP70 Heat-Shock Proteins
Hot Temperature
Calcium
Serotonin
Heat-Shock Response
Protein Synthesis Inhibitors
Quercetin
Heat-Shock Proteins
Thrombin
Dexamethasone
Peptide Hydrolases

Keywords

  • 5-HT-2A receptors
  • Calcium
  • Heat shock protein
  • Quercetin
  • Serotonin

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Kagaya, A., Okada, A., Jitsuiki, H., Tawara, Y., Inagaki, M., Takebayashi, M., ... Yamawaki, S. (2000). Effect of heat stress on serotonin-2A receptor-mediated intracellular calcium mobilization in rat C6 glioma cells. Journal of Neural Transmission, 107(8-9), 919-929. https://doi.org/10.1007/s007020070042

Effect of heat stress on serotonin-2A receptor-mediated intracellular calcium mobilization in rat C6 glioma cells. / Kagaya, A.; Okada, A.; Jitsuiki, H.; Tawara, Y.; Inagaki, M.; Takebayashi, M.; Saeki, T.; Nishida, A.; Nakata, Y.; Yamawaki, S.

In: Journal of Neural Transmission, Vol. 107, No. 8-9, 2000, p. 919-929.

Research output: Contribution to journalArticle

Kagaya, A, Okada, A, Jitsuiki, H, Tawara, Y, Inagaki, M, Takebayashi, M, Saeki, T, Nishida, A, Nakata, Y & Yamawaki, S 2000, 'Effect of heat stress on serotonin-2A receptor-mediated intracellular calcium mobilization in rat C6 glioma cells', Journal of Neural Transmission, vol. 107, no. 8-9, pp. 919-929. https://doi.org/10.1007/s007020070042
Kagaya, A. ; Okada, A. ; Jitsuiki, H. ; Tawara, Y. ; Inagaki, M. ; Takebayashi, M. ; Saeki, T. ; Nishida, A. ; Nakata, Y. ; Yamawaki, S. / Effect of heat stress on serotonin-2A receptor-mediated intracellular calcium mobilization in rat C6 glioma cells. In: Journal of Neural Transmission. 2000 ; Vol. 107, No. 8-9. pp. 919-929.
@article{42ab1aa39da344e99eaabe0e45609489,
title = "Effect of heat stress on serotonin-2A receptor-mediated intracellular calcium mobilization in rat C6 glioma cells",
abstract = "This study was conducted to investigate an effect of heat stress at 44 °C for 30min on intracellular Ca 2+ signaling system and on heat shock protein (HSP)-70 expression. 5-HT-induced Ca 2+ mobilization was reduced 1, 3 and 6hrs after heat stress, and recovered to the control level 12 and 24hrs after heat stress. One hr after heat stress, Ca 2+ rise was significantly decreased when the cells were stimulated by any concentration of 5-HT. Thrombin-induced Ca 2+ increase was also markedly reduced 1hr after heat stress. HSP-70 level was increased 6 and 9 hr after heat stress. In HSP synthesis inhibitor quercetin-treated cells, HSP-70 expression was not enhanced after heat stress, and Ca 2+ rise in response to 5-HT did not return to the control level. However, the Ca 2+ rise induced by 5-HT was not restored to the control level after stress in Ac-Asp-Glu-Val-Asp-H (DEVD)-exposed cells while DEVD had little effect on heat stress-induced synthesis of HSP-70. Dexamethasone did not alter the change in HSP-70 expression or Ca 2+ response after heat stress. These results indicate that heat stress attenuated 5-HT-induced Ca 2+ mobilization and that HSP-70 expression played an important role in recovery from Ca 2+ impairment, possibly via protease activity in C6 cells.",
keywords = "5-HT-2A receptors, Calcium, Heat shock protein, Quercetin, Serotonin",
author = "A. Kagaya and A. Okada and H. Jitsuiki and Y. Tawara and M. Inagaki and M. Takebayashi and T. Saeki and A. Nishida and Y. Nakata and S. Yamawaki",
year = "2000",
doi = "10.1007/s007020070042",
language = "English",
volume = "107",
pages = "919--929",
journal = "Journal of Neural Transmission",
issn = "0300-9564",
publisher = "Springer Verlag",
number = "8-9",

}

TY - JOUR

T1 - Effect of heat stress on serotonin-2A receptor-mediated intracellular calcium mobilization in rat C6 glioma cells

AU - Kagaya, A.

AU - Okada, A.

AU - Jitsuiki, H.

AU - Tawara, Y.

AU - Inagaki, M.

AU - Takebayashi, M.

AU - Saeki, T.

AU - Nishida, A.

AU - Nakata, Y.

AU - Yamawaki, S.

PY - 2000

Y1 - 2000

N2 - This study was conducted to investigate an effect of heat stress at 44 °C for 30min on intracellular Ca 2+ signaling system and on heat shock protein (HSP)-70 expression. 5-HT-induced Ca 2+ mobilization was reduced 1, 3 and 6hrs after heat stress, and recovered to the control level 12 and 24hrs after heat stress. One hr after heat stress, Ca 2+ rise was significantly decreased when the cells were stimulated by any concentration of 5-HT. Thrombin-induced Ca 2+ increase was also markedly reduced 1hr after heat stress. HSP-70 level was increased 6 and 9 hr after heat stress. In HSP synthesis inhibitor quercetin-treated cells, HSP-70 expression was not enhanced after heat stress, and Ca 2+ rise in response to 5-HT did not return to the control level. However, the Ca 2+ rise induced by 5-HT was not restored to the control level after stress in Ac-Asp-Glu-Val-Asp-H (DEVD)-exposed cells while DEVD had little effect on heat stress-induced synthesis of HSP-70. Dexamethasone did not alter the change in HSP-70 expression or Ca 2+ response after heat stress. These results indicate that heat stress attenuated 5-HT-induced Ca 2+ mobilization and that HSP-70 expression played an important role in recovery from Ca 2+ impairment, possibly via protease activity in C6 cells.

AB - This study was conducted to investigate an effect of heat stress at 44 °C for 30min on intracellular Ca 2+ signaling system and on heat shock protein (HSP)-70 expression. 5-HT-induced Ca 2+ mobilization was reduced 1, 3 and 6hrs after heat stress, and recovered to the control level 12 and 24hrs after heat stress. One hr after heat stress, Ca 2+ rise was significantly decreased when the cells were stimulated by any concentration of 5-HT. Thrombin-induced Ca 2+ increase was also markedly reduced 1hr after heat stress. HSP-70 level was increased 6 and 9 hr after heat stress. In HSP synthesis inhibitor quercetin-treated cells, HSP-70 expression was not enhanced after heat stress, and Ca 2+ rise in response to 5-HT did not return to the control level. However, the Ca 2+ rise induced by 5-HT was not restored to the control level after stress in Ac-Asp-Glu-Val-Asp-H (DEVD)-exposed cells while DEVD had little effect on heat stress-induced synthesis of HSP-70. Dexamethasone did not alter the change in HSP-70 expression or Ca 2+ response after heat stress. These results indicate that heat stress attenuated 5-HT-induced Ca 2+ mobilization and that HSP-70 expression played an important role in recovery from Ca 2+ impairment, possibly via protease activity in C6 cells.

KW - 5-HT-2A receptors

KW - Calcium

KW - Heat shock protein

KW - Quercetin

KW - Serotonin

UR - http://www.scopus.com/inward/record.url?scp=0033791872&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033791872&partnerID=8YFLogxK

U2 - 10.1007/s007020070042

DO - 10.1007/s007020070042

M3 - Article

C2 - 11041272

AN - SCOPUS:0033791872

VL - 107

SP - 919

EP - 929

JO - Journal of Neural Transmission

JF - Journal of Neural Transmission

SN - 0300-9564

IS - 8-9

ER -