Effect of heat stress on serotonin-2A receptor-mediated intracellular calcium mobilization in rat C6 glioma cells

A. Kagaya, A. Okada, H. Jitsuiki, Y. Tawara, M. Inagaki, M. Takebayashi, T. Saeki, A. Nishida, Y. Nakata, S. Yamawaki

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4 Citations (Scopus)

Abstract

This study was conducted to investigate an effect of heat stress at 44 °C for 30min on intracellular Ca 2+ signaling system and on heat shock protein (HSP)-70 expression. 5-HT-induced Ca 2+ mobilization was reduced 1, 3 and 6hrs after heat stress, and recovered to the control level 12 and 24hrs after heat stress. One hr after heat stress, Ca 2+ rise was significantly decreased when the cells were stimulated by any concentration of 5-HT. Thrombin-induced Ca 2+ increase was also markedly reduced 1hr after heat stress. HSP-70 level was increased 6 and 9 hr after heat stress. In HSP synthesis inhibitor quercetin-treated cells, HSP-70 expression was not enhanced after heat stress, and Ca 2+ rise in response to 5-HT did not return to the control level. However, the Ca 2+ rise induced by 5-HT was not restored to the control level after stress in Ac-Asp-Glu-Val-Asp-H (DEVD)-exposed cells while DEVD had little effect on heat stress-induced synthesis of HSP-70. Dexamethasone did not alter the change in HSP-70 expression or Ca 2+ response after heat stress. These results indicate that heat stress attenuated 5-HT-induced Ca 2+ mobilization and that HSP-70 expression played an important role in recovery from Ca 2+ impairment, possibly via protease activity in C6 cells.

Original languageEnglish
Pages (from-to)919-929
Number of pages11
JournalJournal of Neural Transmission
Volume107
Issue number8-9
DOIs
Publication statusPublished - Oct 30 2000

Keywords

  • 5-HT-2A receptors
  • Calcium
  • Heat shock protein
  • Quercetin
  • Serotonin

ASJC Scopus subject areas

  • Neurology
  • Clinical Neurology
  • Psychiatry and Mental health
  • Biological Psychiatry

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  • Cite this

    Kagaya, A., Okada, A., Jitsuiki, H., Tawara, Y., Inagaki, M., Takebayashi, M., Saeki, T., Nishida, A., Nakata, Y., & Yamawaki, S. (2000). Effect of heat stress on serotonin-2A receptor-mediated intracellular calcium mobilization in rat C6 glioma cells. Journal of Neural Transmission, 107(8-9), 919-929. https://doi.org/10.1007/s007020070042