Effect of geometry and microstructure of honeycomb TCP scaffolds on bone regeneration

Kiyofumi Takabatake, Eiki Yamachika, Hidetsugu Tsujigiwa, Yasushi Takeda, Mariko Kimura, Shin Takagi, Hitoshi Nagatsuka, Seiji Iida

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

In recent years, artificial biological materials have been commonly used for the treatment of bone tissue defects caused by trauma, tumors, or surgical stress. Although tricalcium phosphate (TCP) is a promising absorbent bone tissue reconstruction biomaterial, it has been reported that its biocompatibility and osteoconductivity depend on its preparation method and sintering temperature. In addition, although it is thought that the microenvironment produced by the extracellular matrix plays an important role in cell growth and differentiation, there have been few studies on how the geometric structure of artificial biological materials affects cells. In the present study, a new honeycomb TCP scaffold containing through-holes with diameters of 300 μm has been developed. The influence of the sintering temperature on the crystal structure and material properties of the honeycomb TCP scaffold was investigated using scanning electron microscopy and X-ray diffraction. Its biocompatibility and osteoconductivity were also evaluated by implantation into experimental animals. It was found that a β-TCP scaffold sintered at 1200°C exhibited high biocompatibility and osteoconductivity, and when it was loaded with BMP-2, it exhibited both osteoconductivity and osteoinductivity, promoting rapid bone formation in both ectopic and orthotopic areas. It is thus a highly promising bone reconstruction material that is expected to find clinical applications.

Original languageEnglish
Pages (from-to)2952-2960
Number of pages9
JournalJournal of Biomedical Materials Research - Part A
Volume102
Issue number9
DOIs
Publication statusPublished - 2014

Fingerprint

Bone Regeneration
Scaffolds
Bone
Phosphates
Biocompatibility
Microstructure
Geometry
Bone and Bones
Biological materials
Sintering
Tissue
Temperature
Cell growth
Biocompatible Materials
Scaffolds (biology)
Osteogenesis
Biomaterials
X-Ray Diffraction
Electron Scanning Microscopy
Extracellular Matrix

Keywords

  • bone
  • geometric structure
  • honeycomb TCP
  • scaffold
  • sintering temperature

ASJC Scopus subject areas

  • Metals and Alloys
  • Biomedical Engineering
  • Biomaterials
  • Ceramics and Composites
  • Medicine(all)

Cite this

Effect of geometry and microstructure of honeycomb TCP scaffolds on bone regeneration. / Takabatake, Kiyofumi; Yamachika, Eiki; Tsujigiwa, Hidetsugu; Takeda, Yasushi; Kimura, Mariko; Takagi, Shin; Nagatsuka, Hitoshi; Iida, Seiji.

In: Journal of Biomedical Materials Research - Part A, Vol. 102, No. 9, 2014, p. 2952-2960.

Research output: Contribution to journalArticle

@article{d9fd4dbfca9645fa9b81292ce666ab8a,
title = "Effect of geometry and microstructure of honeycomb TCP scaffolds on bone regeneration",
abstract = "In recent years, artificial biological materials have been commonly used for the treatment of bone tissue defects caused by trauma, tumors, or surgical stress. Although tricalcium phosphate (TCP) is a promising absorbent bone tissue reconstruction biomaterial, it has been reported that its biocompatibility and osteoconductivity depend on its preparation method and sintering temperature. In addition, although it is thought that the microenvironment produced by the extracellular matrix plays an important role in cell growth and differentiation, there have been few studies on how the geometric structure of artificial biological materials affects cells. In the present study, a new honeycomb TCP scaffold containing through-holes with diameters of 300 μm has been developed. The influence of the sintering temperature on the crystal structure and material properties of the honeycomb TCP scaffold was investigated using scanning electron microscopy and X-ray diffraction. Its biocompatibility and osteoconductivity were also evaluated by implantation into experimental animals. It was found that a β-TCP scaffold sintered at 1200°C exhibited high biocompatibility and osteoconductivity, and when it was loaded with BMP-2, it exhibited both osteoconductivity and osteoinductivity, promoting rapid bone formation in both ectopic and orthotopic areas. It is thus a highly promising bone reconstruction material that is expected to find clinical applications.",
keywords = "bone, geometric structure, honeycomb TCP, scaffold, sintering temperature",
author = "Kiyofumi Takabatake and Eiki Yamachika and Hidetsugu Tsujigiwa and Yasushi Takeda and Mariko Kimura and Shin Takagi and Hitoshi Nagatsuka and Seiji Iida",
year = "2014",
doi = "10.1002/jbm.a.34966",
language = "English",
volume = "102",
pages = "2952--2960",
journal = "Journal of Biomedical Materials Research - Part A",
issn = "1549-3296",
publisher = "John Wiley and Sons Inc.",
number = "9",

}

TY - JOUR

T1 - Effect of geometry and microstructure of honeycomb TCP scaffolds on bone regeneration

AU - Takabatake, Kiyofumi

AU - Yamachika, Eiki

AU - Tsujigiwa, Hidetsugu

AU - Takeda, Yasushi

AU - Kimura, Mariko

AU - Takagi, Shin

AU - Nagatsuka, Hitoshi

AU - Iida, Seiji

PY - 2014

Y1 - 2014

N2 - In recent years, artificial biological materials have been commonly used for the treatment of bone tissue defects caused by trauma, tumors, or surgical stress. Although tricalcium phosphate (TCP) is a promising absorbent bone tissue reconstruction biomaterial, it has been reported that its biocompatibility and osteoconductivity depend on its preparation method and sintering temperature. In addition, although it is thought that the microenvironment produced by the extracellular matrix plays an important role in cell growth and differentiation, there have been few studies on how the geometric structure of artificial biological materials affects cells. In the present study, a new honeycomb TCP scaffold containing through-holes with diameters of 300 μm has been developed. The influence of the sintering temperature on the crystal structure and material properties of the honeycomb TCP scaffold was investigated using scanning electron microscopy and X-ray diffraction. Its biocompatibility and osteoconductivity were also evaluated by implantation into experimental animals. It was found that a β-TCP scaffold sintered at 1200°C exhibited high biocompatibility and osteoconductivity, and when it was loaded with BMP-2, it exhibited both osteoconductivity and osteoinductivity, promoting rapid bone formation in both ectopic and orthotopic areas. It is thus a highly promising bone reconstruction material that is expected to find clinical applications.

AB - In recent years, artificial biological materials have been commonly used for the treatment of bone tissue defects caused by trauma, tumors, or surgical stress. Although tricalcium phosphate (TCP) is a promising absorbent bone tissue reconstruction biomaterial, it has been reported that its biocompatibility and osteoconductivity depend on its preparation method and sintering temperature. In addition, although it is thought that the microenvironment produced by the extracellular matrix plays an important role in cell growth and differentiation, there have been few studies on how the geometric structure of artificial biological materials affects cells. In the present study, a new honeycomb TCP scaffold containing through-holes with diameters of 300 μm has been developed. The influence of the sintering temperature on the crystal structure and material properties of the honeycomb TCP scaffold was investigated using scanning electron microscopy and X-ray diffraction. Its biocompatibility and osteoconductivity were also evaluated by implantation into experimental animals. It was found that a β-TCP scaffold sintered at 1200°C exhibited high biocompatibility and osteoconductivity, and when it was loaded with BMP-2, it exhibited both osteoconductivity and osteoinductivity, promoting rapid bone formation in both ectopic and orthotopic areas. It is thus a highly promising bone reconstruction material that is expected to find clinical applications.

KW - bone

KW - geometric structure

KW - honeycomb TCP

KW - scaffold

KW - sintering temperature

UR - http://www.scopus.com/inward/record.url?scp=84904822762&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84904822762&partnerID=8YFLogxK

U2 - 10.1002/jbm.a.34966

DO - 10.1002/jbm.a.34966

M3 - Article

C2 - 24115688

AN - SCOPUS:84904822762

VL - 102

SP - 2952

EP - 2960

JO - Journal of Biomedical Materials Research - Part A

JF - Journal of Biomedical Materials Research - Part A

SN - 1549-3296

IS - 9

ER -