TY - JOUR
T1 - Effect of formaldehyde gas exposure in a murine allergic contact hypersensitivity model
AU - Fujii, Kazuyasu
AU - Tsuji, Kazuhide
AU - Matsuura, Hironori
AU - Okazaki, Fusako
AU - Takahashi, Sachiko
AU - Arata, Jirô
AU - Iwatsuki, Keiji
N1 - Funding Information:
This work was partly supported by Health and Labour Sciences Research Grants, Research on Health Services; ‘‘Sick House Syndrome (SHS): Studies of patho-physiology, method of diagnosis and treatment (H12-Seikatsu-005)’’; and by the Long-range Research Initiative (LRI) Grants 2003CS04 from the Japan Chemical Industry Association (JCIA).
PY - 2005
Y1 - 2005
N2 - To clarify the effect of formaldehyde (FA) gas exposure on contact hypersensitivity (CHS), CHS reactions against 2,4,6-trinitrochlorobenzene (TNCB) was studied in BALB/c mice with a low dose of FA gas exposure. The TNCB-induced CHS reactions were slightly suppressed by the FA gas exposure immediately after sensitization, whereas they were significantly enhanced and prolonged in mice continuously exposed to FA gas before and after sensitization. We showed that exposure to FA gas enhanced the Th2 dominant responses in draining lymph node (LN) in early stage of CHS. In contrast, T cell subsets and their intracellular cytokine production in the draining LN were similar during the early stage of CHS by FA gas exposure during the sensitization phase. The percentage of CD8+ T cells was increased, and the percentage of CD4 +CD25+ T cells was decreased in the FA gas-exposed group at 72 hr after elicitation. These results indicate that FA gas-exposed might influence regulatory T cells. Furthermore, in the chronic CHS model that was repetitively elicited with TNCB, more intensive and prolonged CHS reactions, and increased numbers of mast cells were found in the FA gas-exposed group at 4 hr after elicitation than in the control group, FA gas exposure may alter the intensity of allergic CHS.
AB - To clarify the effect of formaldehyde (FA) gas exposure on contact hypersensitivity (CHS), CHS reactions against 2,4,6-trinitrochlorobenzene (TNCB) was studied in BALB/c mice with a low dose of FA gas exposure. The TNCB-induced CHS reactions were slightly suppressed by the FA gas exposure immediately after sensitization, whereas they were significantly enhanced and prolonged in mice continuously exposed to FA gas before and after sensitization. We showed that exposure to FA gas enhanced the Th2 dominant responses in draining lymph node (LN) in early stage of CHS. In contrast, T cell subsets and their intracellular cytokine production in the draining LN were similar during the early stage of CHS by FA gas exposure during the sensitization phase. The percentage of CD8+ T cells was increased, and the percentage of CD4 +CD25+ T cells was decreased in the FA gas-exposed group at 72 hr after elicitation. These results indicate that FA gas-exposed might influence regulatory T cells. Furthermore, in the chronic CHS model that was repetitively elicited with TNCB, more intensive and prolonged CHS reactions, and increased numbers of mast cells were found in the FA gas-exposed group at 4 hr after elicitation than in the control group, FA gas exposure may alter the intensity of allergic CHS.
KW - Contact hypersensitivity
KW - Formaldehyde gas exposure
KW - Sick-building syndrome
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U2 - 10.1081/IPH-51768
DO - 10.1081/IPH-51768
M3 - Article
C2 - 15803868
AN - SCOPUS:15244351023
VL - 27
SP - 163
EP - 175
JO - Immunopharmacology and Immunotoxicology
JF - Immunopharmacology and Immunotoxicology
SN - 0892-3973
IS - 1
ER -