Effect of Estradiol and Nonylphenol on mRNA Expression of Estrogen Receptors α and β, and Cytochrome P450 Aromatase in the Gonad of Chicken Embryos

Masanori Sakimura, Akira Tsukada, Noboru Saito, Kiyoshi Shimada, Makoto Usami, Yasuo Ohno, Shozo Hanzawa

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Effects of estradiol-17β (E2) and p-nonylphenol (NP) on mRNA expression of estrogen receptor α (ERα), estrogen receptor β (ERβ) and cytochrome P450 aromatase (P450arom) were examined in the early stage of chicken embryonic gonads. Fertilized eggs were treated with either E2 (1.0mg/egg) or NP (0.01, O.1mg/egg) immediately before incubation (day 0 of incubation) and were incubated at 37.5°C. In the control group the eggs were treated with the vehicle (10% propanediol, PD, 50μl). On day 10 of incubation the gonad was collected and mRNA expression was determined for semi-quantitative assay by the reverse transcription-polymerase chain reaction (RT-PCR). In the control group, ERα and P450arom mRNA showed higher expression in females than in males while ERβ mRNA showed no sexual difference. In the E2 treated group, the expression of ERα and P450arom mRNA markedly increased in males (about 4- and 16-fold, respectively). NP increased mRNA expression of ERα and P450 arom (about 2-fold each) in males. ERβ mRNA expression did not apparently change after the E2 or NP treatment in both sexes. These results suggest that NP induces some female-specific genes in the male gonads during the critical period of sex determination in chicken embryos.

Original languageEnglish
Pages (from-to)302-309
Number of pages8
JournalJournal of Poultry Science
Volume39
Issue number4
DOIs
Publication statusPublished - 2002
Externally publishedYes

Fingerprint

cytochrome P-450
estradiol
gonads
embryo (animal)
chickens
propanediols
gender
estrogen receptors
aromatase
reverse transcriptase polymerase chain reaction
assays
genes

Keywords

  • endocrine disruptor
  • ERα
  • ERβ
  • p-nonylphenol
  • P450arom

ASJC Scopus subject areas

  • Animal Science and Zoology

Cite this

Effect of Estradiol and Nonylphenol on mRNA Expression of Estrogen Receptors α and β, and Cytochrome P450 Aromatase in the Gonad of Chicken Embryos. / Sakimura, Masanori; Tsukada, Akira; Saito, Noboru; Shimada, Kiyoshi; Usami, Makoto; Ohno, Yasuo; Hanzawa, Shozo.

In: Journal of Poultry Science, Vol. 39, No. 4, 2002, p. 302-309.

Research output: Contribution to journalArticle

Sakimura, Masanori ; Tsukada, Akira ; Saito, Noboru ; Shimada, Kiyoshi ; Usami, Makoto ; Ohno, Yasuo ; Hanzawa, Shozo. / Effect of Estradiol and Nonylphenol on mRNA Expression of Estrogen Receptors α and β, and Cytochrome P450 Aromatase in the Gonad of Chicken Embryos. In: Journal of Poultry Science. 2002 ; Vol. 39, No. 4. pp. 302-309.
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