Effect of endotoxin-induced reactive oxygen species on sperm motility

Kenichiro Urata, Hisashi Narahara, Yuichiro Tanaka, Toru Egashira, Fusako Takayama, Isao Miyakawa

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

Objective: To define the mechanism of infection-induced damage of sperm. Design: The effect of lipopolysaccharide (LPS)-induced reactive oxygen species (ROS) on sperm motility and its modification by scavengers were investigated. Setting: Research laboratory of a university hospital. Patient(s): Normozoospermic semen samples were obtained from 37 healthy volunteers. Intervention(s): The sperms were incubated in the presence of LPS with or without scavengers. Main Outcome Measure(s): Sperm motility was evaluated by a sperm quality analyzer (SQAIIB). ROS formation in semen samples was measured by a Berthold luminometer (LB953). Result(s): Motility of spermatozoa was decreased in the LPS-treated samples compared with that in the control groups. ROS was significantly higher in the LPS-treated groups than in the control groups. The addition of ROS scavengers restored the motility index and suppressed ROS production in the LPS-treated semen samples. Conclusion(s): These data suggest that endotoxin-induced excessive production of ROS is responsible for the decrease in sperm motility and that antioxidant therapy may be a therapeutic option for infertile men with bacterial genital tract infection.

Original languageEnglish
Pages (from-to)163-166
Number of pages4
JournalFertility and Sterility
Volume76
Issue number1
DOIs
Publication statusPublished - 2001
Externally publishedYes

Fingerprint

Sperm Motility
Endotoxins
Reactive Oxygen Species
Lipopolysaccharides
Spermatozoa
Semen
Reproductive Tract Infections
Control Groups
Healthy Volunteers
Antioxidants
Outcome Assessment (Health Care)
Therapeutics
Infection
Research

Keywords

  • Lipopolysaccharide
  • Reactive oxygen species
  • Scavengers
  • Sperm
  • Sperm motility

ASJC Scopus subject areas

  • Obstetrics and Gynaecology

Cite this

Effect of endotoxin-induced reactive oxygen species on sperm motility. / Urata, Kenichiro; Narahara, Hisashi; Tanaka, Yuichiro; Egashira, Toru; Takayama, Fusako; Miyakawa, Isao.

In: Fertility and Sterility, Vol. 76, No. 1, 2001, p. 163-166.

Research output: Contribution to journalArticle

Urata, K, Narahara, H, Tanaka, Y, Egashira, T, Takayama, F & Miyakawa, I 2001, 'Effect of endotoxin-induced reactive oxygen species on sperm motility', Fertility and Sterility, vol. 76, no. 1, pp. 163-166. https://doi.org/10.1016/S0015-0282(01)01850-7
Urata, Kenichiro ; Narahara, Hisashi ; Tanaka, Yuichiro ; Egashira, Toru ; Takayama, Fusako ; Miyakawa, Isao. / Effect of endotoxin-induced reactive oxygen species on sperm motility. In: Fertility and Sterility. 2001 ; Vol. 76, No. 1. pp. 163-166.
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