TY - JOUR
T1 - Effect of docosahexaenoic acid on in vitro growth of bovine oocytes
AU - Nagata, Shuta
AU - Tatematsu, Kaoru
AU - Yamaguchi, Hitoki
AU - Inoue, Yuki
AU - Tanaka, Keisuke
AU - Tasaki, Hidetaka
AU - Shirasuna, Koumei
AU - Iwata, Hisataka
N1 - Funding Information:
This study was supported by JSPS KAKENHI (grant number 16K07996).
Publisher Copyright:
© 2021 The Authors. Reproductive Medicine and Biology published by John Wiley & Sons Australia, Ltd on behalf of Japan Society for Reproductive Medicine.
PY - 2021/10
Y1 - 2021/10
N2 - Purpose: The present study investigated the effects of docosahexaenoic acid (DHA) on the growth of bovine oocytes. Methods: Oocytes and granulosa cell complexes (OGCs) were collected from early antral follicles (0.4-0.7 mm) on the surface of ovaries harvested from a slaughterhouse. The OGCs were cultured with 0, 1, and 10 μmol/L docosahexanoic acid (DHA) for 16 days. Results: Antrum formation of the OGCs and the number of granulosa cells (GCs) surrounding the oocytes were comparable among groups, whereas supplementation of 0.1 μmol/L of DHA significantly improved oocyte growth. Oocytes grown with DHA had a higher fertilization rate, acetylation levels of H4K12, and ATP contents, as well as a lower lipid content compared with those grown without DHA. In addition, GCs surrounding OGCs grown with DHA had low lipid content compared with vehicle counterparts. Furthermore, when GCs were cultured in vitro, DHA increased ATP production, mitochondrial membrane potential, and reduced lipid content and levels of reactive oxygen species. RNA-seq of GCs revealed that DHA increased CPT1A expression levels and affect genes associated with focal adhesion, oxidative phosphorylation, and PI3K-AKT etc. Conclusion: The results suggest that DHA supplementation affects granulosa cell characteristics and supports oocyte growth in vitro.
AB - Purpose: The present study investigated the effects of docosahexaenoic acid (DHA) on the growth of bovine oocytes. Methods: Oocytes and granulosa cell complexes (OGCs) were collected from early antral follicles (0.4-0.7 mm) on the surface of ovaries harvested from a slaughterhouse. The OGCs were cultured with 0, 1, and 10 μmol/L docosahexanoic acid (DHA) for 16 days. Results: Antrum formation of the OGCs and the number of granulosa cells (GCs) surrounding the oocytes were comparable among groups, whereas supplementation of 0.1 μmol/L of DHA significantly improved oocyte growth. Oocytes grown with DHA had a higher fertilization rate, acetylation levels of H4K12, and ATP contents, as well as a lower lipid content compared with those grown without DHA. In addition, GCs surrounding OGCs grown with DHA had low lipid content compared with vehicle counterparts. Furthermore, when GCs were cultured in vitro, DHA increased ATP production, mitochondrial membrane potential, and reduced lipid content and levels of reactive oxygen species. RNA-seq of GCs revealed that DHA increased CPT1A expression levels and affect genes associated with focal adhesion, oxidative phosphorylation, and PI3K-AKT etc. Conclusion: The results suggest that DHA supplementation affects granulosa cell characteristics and supports oocyte growth in vitro.
KW - ATP
KW - DHA
KW - lipid
KW - oocyte growth
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U2 - 10.1002/rmb2.12403
DO - 10.1002/rmb2.12403
M3 - Article
AN - SCOPUS:85109363038
SN - 1445-5781
VL - 20
SP - 485
EP - 493
JO - Reproductive Medicine and Biology
JF - Reproductive Medicine and Biology
IS - 4
ER -