Effect of diglucosamine on the entrapment of protein into liposomes

S. Murakami, Tsutomu Ono, S. Sakai, H. Ijima, K. Kawakami

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Liposomes, which had entrapped bovine serum albumin (BSA), were modified with diglucosamine by two methods. The liposome was prepared by a freeze-thawing method in the presence of the disaccharide, or the disaccharide was added to the liposome prepared in advance without it. To examine the effects of diglucosamine, the morphology, mean particle size, and zeta potential of both liposomes were compared with those of BSA-entrapping liposome prepared without the disaccharide. Diglucosamine caused no remarkable change in shape and no aggregation of the liposome. The presence of the disaccharide was confirmed on the surfaces of modified liposomes, and the entrapment of BSA into the liposomes was increased by the disaccharide. The entrapment behavior was affected by the way the disaccharide was added, and the difference in the way the BSA was entrapped was also indicated.

Original languageEnglish
Pages (from-to)103-112
Number of pages10
JournalJournal of Liposome Research
Volume16
Issue number2
DOIs
Publication statusPublished - Jul 2006
Externally publishedYes

Fingerprint

Liposomes
Disaccharides
Bovine Serum Albumin
Proteins
Particle Size

Keywords

  • Bovine serum albumin
  • Diglucosamine
  • Entrapment
  • Freeze-thawing method
  • Liposomes

ASJC Scopus subject areas

  • Pharmacology

Cite this

Effect of diglucosamine on the entrapment of protein into liposomes. / Murakami, S.; Ono, Tsutomu; Sakai, S.; Ijima, H.; Kawakami, K.

In: Journal of Liposome Research, Vol. 16, No. 2, 07.2006, p. 103-112.

Research output: Contribution to journalArticle

Murakami, S. ; Ono, Tsutomu ; Sakai, S. ; Ijima, H. ; Kawakami, K. / Effect of diglucosamine on the entrapment of protein into liposomes. In: Journal of Liposome Research. 2006 ; Vol. 16, No. 2. pp. 103-112.
@article{c7731179d1cf458bbc437f0eaad8dbc4,
title = "Effect of diglucosamine on the entrapment of protein into liposomes",
abstract = "Liposomes, which had entrapped bovine serum albumin (BSA), were modified with diglucosamine by two methods. The liposome was prepared by a freeze-thawing method in the presence of the disaccharide, or the disaccharide was added to the liposome prepared in advance without it. To examine the effects of diglucosamine, the morphology, mean particle size, and zeta potential of both liposomes were compared with those of BSA-entrapping liposome prepared without the disaccharide. Diglucosamine caused no remarkable change in shape and no aggregation of the liposome. The presence of the disaccharide was confirmed on the surfaces of modified liposomes, and the entrapment of BSA into the liposomes was increased by the disaccharide. The entrapment behavior was affected by the way the disaccharide was added, and the difference in the way the BSA was entrapped was also indicated.",
keywords = "Bovine serum albumin, Diglucosamine, Entrapment, Freeze-thawing method, Liposomes",
author = "S. Murakami and Tsutomu Ono and S. Sakai and H. Ijima and K. Kawakami",
year = "2006",
month = "7",
doi = "10.1080/08982100600680667",
language = "English",
volume = "16",
pages = "103--112",
journal = "Journal of Liposome Research",
issn = "0898-2104",
publisher = "Informa Healthcare",
number = "2",

}

TY - JOUR

T1 - Effect of diglucosamine on the entrapment of protein into liposomes

AU - Murakami, S.

AU - Ono, Tsutomu

AU - Sakai, S.

AU - Ijima, H.

AU - Kawakami, K.

PY - 2006/7

Y1 - 2006/7

N2 - Liposomes, which had entrapped bovine serum albumin (BSA), were modified with diglucosamine by two methods. The liposome was prepared by a freeze-thawing method in the presence of the disaccharide, or the disaccharide was added to the liposome prepared in advance without it. To examine the effects of diglucosamine, the morphology, mean particle size, and zeta potential of both liposomes were compared with those of BSA-entrapping liposome prepared without the disaccharide. Diglucosamine caused no remarkable change in shape and no aggregation of the liposome. The presence of the disaccharide was confirmed on the surfaces of modified liposomes, and the entrapment of BSA into the liposomes was increased by the disaccharide. The entrapment behavior was affected by the way the disaccharide was added, and the difference in the way the BSA was entrapped was also indicated.

AB - Liposomes, which had entrapped bovine serum albumin (BSA), were modified with diglucosamine by two methods. The liposome was prepared by a freeze-thawing method in the presence of the disaccharide, or the disaccharide was added to the liposome prepared in advance without it. To examine the effects of diglucosamine, the morphology, mean particle size, and zeta potential of both liposomes were compared with those of BSA-entrapping liposome prepared without the disaccharide. Diglucosamine caused no remarkable change in shape and no aggregation of the liposome. The presence of the disaccharide was confirmed on the surfaces of modified liposomes, and the entrapment of BSA into the liposomes was increased by the disaccharide. The entrapment behavior was affected by the way the disaccharide was added, and the difference in the way the BSA was entrapped was also indicated.

KW - Bovine serum albumin

KW - Diglucosamine

KW - Entrapment

KW - Freeze-thawing method

KW - Liposomes

UR - http://www.scopus.com/inward/record.url?scp=33744982085&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33744982085&partnerID=8YFLogxK

U2 - 10.1080/08982100600680667

DO - 10.1080/08982100600680667

M3 - Article

C2 - 16753965

AN - SCOPUS:33744982085

VL - 16

SP - 103

EP - 112

JO - Journal of Liposome Research

JF - Journal of Liposome Research

SN - 0898-2104

IS - 2

ER -