Effect of deferoxamine-activated hypoxia inducible factor-1 on the brainstem following subarachnoid haemorrhage

S. Ono, Tomohito Hishikawa, T. Ogawa, M. Nishiguchi, K. Onoda, K. Tokunaga, K. Sugiu, I. Date

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Hypoxia inducible factor-1 (HIF-1) is a transcription factor that regulates the expression of various neuroprotective genes. The goal of this study was to clarify the relationship between HIF-1α expression and subarachnoid haemorrhage (SAH) and to determine the effects of the deferoxamine (DFO)-induced increase in HIF-1α protein levels on the brainstem and the basilar artery (BA) following experimental SAH. Rat single- (10min, 6 h, Day 1, Day 2) and double-haemorrhage (Day 7) models of SAH were used. The time course of HIF-1α protein levels of the brainstems and the diameter of the BA was assessed. After an induction of double-haemorrhage in rats, DFO was injected intraperitoneally and HIF-1α protein expression, activity in the brainstems, the diameter of the BA and the brainstem blood flow were assessed. The expression of HIF-1α protein was significantly greater at 10 min and 7 days after SAH. The increase of HIF-1α protein correlated with the degree of cerebral vasospasm. An injection of DFO resulted in significant increases in HIF-1α protein expression and activity in the brainstem of rats with SAH. These results indicate that DFO-induced increase in HIF-1α protein levels and activity exerts neuroprotective and antivasospastic effects. DFO may be a potential therapeutic tool for cerebral vasospasm after SAH.

Original languageEnglish
Title of host publicationCerebral Vasospasm
Subtitle of host publicationNew Strategies in Research and Treatment
PublisherSpringer-Verlag Wien
Pages69-73
Number of pages5
Edition104
ISBN (Print)9783211757178
DOIs
Publication statusPublished - 2008

Publication series

NameActa Neurochirurgica, Supplementum
Number104
ISSN (Print)0065-1419

Keywords

  • Brainstem
  • cerebral vasospasm
  • deferoxamine
  • hypoxia inducible factor-1
  • rats
  • subarachnoid haemorrhage

ASJC Scopus subject areas

  • Surgery
  • Clinical Neurology

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    Ono, S., Hishikawa, T., Ogawa, T., Nishiguchi, M., Onoda, K., Tokunaga, K., Sugiu, K., & Date, I. (2008). Effect of deferoxamine-activated hypoxia inducible factor-1 on the brainstem following subarachnoid haemorrhage. In Cerebral Vasospasm: New Strategies in Research and Treatment (104 ed., pp. 69-73). (Acta Neurochirurgica, Supplementum; No. 104). Springer-Verlag Wien. https://doi.org/10.1007/978-3-211-75718-5_13