Effect of dantrolene on KCl- or NMDA-induced intracellular Ca2+ changes and spontaneous Ca2+ oscillation in cultured rat frontal cortical neurons

T. Hayashi, A. Kagaya, M. Takebayashi, T. Oyamada, M. Inagaki, Y. Tawara, N. Yokota, J. Horiguchi, T. P. Su, S. Yamawaki

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Dantrolene has been known to affect intracellular Ca2+ concentration ([Ca2+](i)) by inhibiting Ca2+ release from intracellular stores in cultured neurons. We were interested in examining this property of dantrolene in influencing the [Ca2+](i) affected by the NMDA receptor ligands, KCl, L-type Ca2+ channel blocker nifedipine, and two other intracellular Ca2+-mobilizing agents caffeine and bradykinin. Effect of dantrolene on the spontaneous oscillation of [Ca2+](i) was also examined. Dantrolene in μM concentrations dose-dependently inhibited the increase in [Ca2+](i) elicited by NMDA and KCl. AP-5, MK-801 (NMDA antagonists), and nifedipine respectively reduced the NMDA and KCl-induced increase in [Ca2+](i). Dantrolene, added to the buffer solution together with the antagonists or nifedipine, caused a further reduction in [Ca2+](i) to a degree similar to that seen with dantrolene alone inhibiting the increase in [Ca2+](i) caused by NMDA or KCl. At 30 μM, dantrolene partially inhibited caffeine-induced increase in [Ca2+](i) whereas it has no effect on the bradykinin-induced change in [Ca2+](i). The spontaneous oscillation of [Ca2+](i) in frontal cortical neurons was reduced both in amplitude and in base line concentration in the presence of 10 μM dantrolene. Our results indicate that dantrolene's mobilizing effects on intracellular Ca2+ stores operate independently from the influxed Ca2+ and that a component of the apparent increase in [Ca2+](i) elicited by NMDA or KCl represents a dantrolene-sensitive Ca2+ release from intracellular stores. Results also suggest that dantrolene does not affect the IP3-gated release of intracellular Ca2+ and that the spontaneous Ca2+ oscillation is, at least partially, under the control of Ca2+ mobilization from internal stores.

Original languageEnglish
Pages (from-to)811-824
Number of pages14
JournalJournal of Neural Transmission
Volume104
Issue number8-9
Publication statusPublished - 1997
Externally publishedYes

Fingerprint

Dantrolene
N-Methylaspartate
Neurons
Nifedipine
Bradykinin
Caffeine
Dizocilpine Maleate
N-Methyl-D-Aspartate Receptors
Buffers

Keywords

  • Ca-induced Ca release
  • Intracellular Ca stores
  • Intracellular free Ca concentration
  • N-methyl-D-aspartate receptor
  • Primary cultures of rat frontal cortical neurons
  • Spontaneous Ca oscillation

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Hayashi, T., Kagaya, A., Takebayashi, M., Oyamada, T., Inagaki, M., Tawara, Y., ... Yamawaki, S. (1997). Effect of dantrolene on KCl- or NMDA-induced intracellular Ca2+ changes and spontaneous Ca2+ oscillation in cultured rat frontal cortical neurons. Journal of Neural Transmission, 104(8-9), 811-824.

Effect of dantrolene on KCl- or NMDA-induced intracellular Ca2+ changes and spontaneous Ca2+ oscillation in cultured rat frontal cortical neurons. / Hayashi, T.; Kagaya, A.; Takebayashi, M.; Oyamada, T.; Inagaki, M.; Tawara, Y.; Yokota, N.; Horiguchi, J.; Su, T. P.; Yamawaki, S.

In: Journal of Neural Transmission, Vol. 104, No. 8-9, 1997, p. 811-824.

Research output: Contribution to journalArticle

Hayashi, T, Kagaya, A, Takebayashi, M, Oyamada, T, Inagaki, M, Tawara, Y, Yokota, N, Horiguchi, J, Su, TP & Yamawaki, S 1997, 'Effect of dantrolene on KCl- or NMDA-induced intracellular Ca2+ changes and spontaneous Ca2+ oscillation in cultured rat frontal cortical neurons', Journal of Neural Transmission, vol. 104, no. 8-9, pp. 811-824.
Hayashi, T. ; Kagaya, A. ; Takebayashi, M. ; Oyamada, T. ; Inagaki, M. ; Tawara, Y. ; Yokota, N. ; Horiguchi, J. ; Su, T. P. ; Yamawaki, S. / Effect of dantrolene on KCl- or NMDA-induced intracellular Ca2+ changes and spontaneous Ca2+ oscillation in cultured rat frontal cortical neurons. In: Journal of Neural Transmission. 1997 ; Vol. 104, No. 8-9. pp. 811-824.
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AU - Kagaya, A.

AU - Takebayashi, M.

AU - Oyamada, T.

AU - Inagaki, M.

AU - Tawara, Y.

AU - Yokota, N.

AU - Horiguchi, J.

AU - Su, T. P.

AU - Yamawaki, S.

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