Effect of D-alanine in teichoic acid from the Streptococcus thermophilus cell wall on the barrier-protection of intestinal epithelial cells

Eiji Miyauchi, Maho Morita, Mauro Rossi, Hidetoshi Morita, Takuya Suzuki, Soichi Tanabe

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

D-Alanylation of teichoic acid (TA) affects various functions of Gram-positive bacteria, including immunomodulatory effects. We investigated in this study the impact of D-alanine (D-Ala) in TA from Streptococcus thermophilus ATCC 19258 T on the barrier-protecting effect in human intestinal Caco-2 cells. ATCC 19258 T suppressed the tumor necrosis factor-α-induced decrease in transepithelial electrical resistance (TER), an indicator of the barrier function. The D-alanylation of TA in ATCC 19258 T was growth phase-and culture temperature-dependent. Treatment of ATCC 19258 T with Mg 2+ decreased the dlt mRNA expression and DAla content in TA and also abolished the suppressive effect on the TER decrease. Supplementation with Lalanine (L-Ala) to the broth led to an increase of D-Ala in ATCC 19258 T and of the intestinal barrier-protecting effect. Taken together, D-Ala in TA played an important role in the barrier-protecting effect of S. thermophilus in the intestinal epithelium, and these beneficial effects could be enhanced by exogenous L-Ala.

Original languageEnglish
Pages (from-to)283-288
Number of pages6
JournalBioscience, Biotechnology and Biochemistry
Volume76
Issue number2
DOIs
Publication statusPublished - 2012
Externally publishedYes

Keywords

  • Caco-2
  • D-alanine
  • Intestinal barrier
  • Streptococcus thermophilus
  • Teichoic acid

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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