Abstract
D-Alanylation of teichoic acid (TA) affects various functions of Gram-positive bacteria, including immunomodulatory effects. We investigated in this study the impact of D-alanine (D-Ala) in TA from Streptococcus thermophilus ATCC 19258 T on the barrier-protecting effect in human intestinal Caco-2 cells. ATCC 19258 T suppressed the tumor necrosis factor-α-induced decrease in transepithelial electrical resistance (TER), an indicator of the barrier function. The D-alanylation of TA in ATCC 19258 T was growth phase-and culture temperature-dependent. Treatment of ATCC 19258 T with Mg 2+ decreased the dlt mRNA expression and DAla content in TA and also abolished the suppressive effect on the TER decrease. Supplementation with Lalanine (L-Ala) to the broth led to an increase of D-Ala in ATCC 19258 T and of the intestinal barrier-protecting effect. Taken together, D-Ala in TA played an important role in the barrier-protecting effect of S. thermophilus in the intestinal epithelium, and these beneficial effects could be enhanced by exogenous L-Ala.
| Original language | English |
|---|---|
| Pages (from-to) | 283-288 |
| Number of pages | 6 |
| Journal | Bioscience, Biotechnology and Biochemistry |
| Volume | 76 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 2012 |
| Externally published | Yes |
Fingerprint
Keywords
- Caco-2
- D-alanine
- Intestinal barrier
- Streptococcus thermophilus
- Teichoic acid
ASJC Scopus subject areas
- Biotechnology
- Biochemistry
- Molecular Biology
- Applied Microbiology and Biotechnology
- Analytical Chemistry
- Organic Chemistry
Cite this
Effect of D-alanine in teichoic acid from the Streptococcus thermophilus cell wall on the barrier-protection of intestinal epithelial cells. / Miyauchi, Eiji; Morita, Maho; Rossi, Mauro; Morita, Hidetoshi; Suzuki, Takuya; Tanabe, Soichi.
In: Bioscience, Biotechnology and Biochemistry, Vol. 76, No. 2, 2012, p. 283-288.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Effect of D-alanine in teichoic acid from the Streptococcus thermophilus cell wall on the barrier-protection of intestinal epithelial cells
AU - Miyauchi, Eiji
AU - Morita, Maho
AU - Rossi, Mauro
AU - Morita, Hidetoshi
AU - Suzuki, Takuya
AU - Tanabe, Soichi
PY - 2012
Y1 - 2012
N2 - D-Alanylation of teichoic acid (TA) affects various functions of Gram-positive bacteria, including immunomodulatory effects. We investigated in this study the impact of D-alanine (D-Ala) in TA from Streptococcus thermophilus ATCC 19258 T on the barrier-protecting effect in human intestinal Caco-2 cells. ATCC 19258 T suppressed the tumor necrosis factor-α-induced decrease in transepithelial electrical resistance (TER), an indicator of the barrier function. The D-alanylation of TA in ATCC 19258 T was growth phase-and culture temperature-dependent. Treatment of ATCC 19258 T with Mg 2+ decreased the dlt mRNA expression and DAla content in TA and also abolished the suppressive effect on the TER decrease. Supplementation with Lalanine (L-Ala) to the broth led to an increase of D-Ala in ATCC 19258 T and of the intestinal barrier-protecting effect. Taken together, D-Ala in TA played an important role in the barrier-protecting effect of S. thermophilus in the intestinal epithelium, and these beneficial effects could be enhanced by exogenous L-Ala.
AB - D-Alanylation of teichoic acid (TA) affects various functions of Gram-positive bacteria, including immunomodulatory effects. We investigated in this study the impact of D-alanine (D-Ala) in TA from Streptococcus thermophilus ATCC 19258 T on the barrier-protecting effect in human intestinal Caco-2 cells. ATCC 19258 T suppressed the tumor necrosis factor-α-induced decrease in transepithelial electrical resistance (TER), an indicator of the barrier function. The D-alanylation of TA in ATCC 19258 T was growth phase-and culture temperature-dependent. Treatment of ATCC 19258 T with Mg 2+ decreased the dlt mRNA expression and DAla content in TA and also abolished the suppressive effect on the TER decrease. Supplementation with Lalanine (L-Ala) to the broth led to an increase of D-Ala in ATCC 19258 T and of the intestinal barrier-protecting effect. Taken together, D-Ala in TA played an important role in the barrier-protecting effect of S. thermophilus in the intestinal epithelium, and these beneficial effects could be enhanced by exogenous L-Ala.
KW - Caco-2
KW - D-alanine
KW - Intestinal barrier
KW - Streptococcus thermophilus
KW - Teichoic acid
UR - http://www.scopus.com/inward/record.url?scp=84863296694&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84863296694&partnerID=8YFLogxK
U2 - 10.1271/bbb.110646
DO - 10.1271/bbb.110646
M3 - Article
C2 - 22313760
AN - SCOPUS:84863296694
VL - 76
SP - 283
EP - 288
JO - Bioscience, Biotechnology and Biochemistry
JF - Bioscience, Biotechnology and Biochemistry
SN - 0916-8451
IS - 2
ER -