Abstract
D-Alanylation of teichoic acid (TA) affects various functions of Gram-positive bacteria, including immunomodulatory effects. We investigated in this study the impact of D-alanine (D-Ala) in TA from Streptococcus thermophilus ATCC 19258 T on the barrier-protecting effect in human intestinal Caco-2 cells. ATCC 19258 T suppressed the tumor necrosis factor-α-induced decrease in transepithelial electrical resistance (TER), an indicator of the barrier function. The D-alanylation of TA in ATCC 19258 T was growth phase-and culture temperature-dependent. Treatment of ATCC 19258 T with Mg 2+ decreased the dlt mRNA expression and DAla content in TA and also abolished the suppressive effect on the TER decrease. Supplementation with Lalanine (L-Ala) to the broth led to an increase of D-Ala in ATCC 19258 T and of the intestinal barrier-protecting effect. Taken together, D-Ala in TA played an important role in the barrier-protecting effect of S. thermophilus in the intestinal epithelium, and these beneficial effects could be enhanced by exogenous L-Ala.
Original language | English |
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Pages (from-to) | 283-288 |
Number of pages | 6 |
Journal | Bioscience, Biotechnology and Biochemistry |
Volume | 76 |
Issue number | 2 |
DOIs | |
Publication status | Published - 2012 |
Externally published | Yes |
Keywords
- Caco-2
- D-alanine
- Intestinal barrier
- Streptococcus thermophilus
- Teichoic acid
ASJC Scopus subject areas
- Biotechnology
- Analytical Chemistry
- Biochemistry
- Applied Microbiology and Biotechnology
- Molecular Biology
- Organic Chemistry