Effect of D-alanine in teichoic acid from the Streptococcus thermophilus cell wall on the barrier-protection of intestinal epithelial cells

Eiji Miyauchi, Maho Morita, Mauro Rossi, Hidetoshi Morita, Takuya Suzuki, Soichi Tanabe

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

D-Alanylation of teichoic acid (TA) affects various functions of Gram-positive bacteria, including immunomodulatory effects. We investigated in this study the impact of D-alanine (D-Ala) in TA from Streptococcus thermophilus ATCC 19258 T on the barrier-protecting effect in human intestinal Caco-2 cells. ATCC 19258 T suppressed the tumor necrosis factor-α-induced decrease in transepithelial electrical resistance (TER), an indicator of the barrier function. The D-alanylation of TA in ATCC 19258 T was growth phase-and culture temperature-dependent. Treatment of ATCC 19258 T with Mg 2+ decreased the dlt mRNA expression and DAla content in TA and also abolished the suppressive effect on the TER decrease. Supplementation with Lalanine (L-Ala) to the broth led to an increase of D-Ala in ATCC 19258 T and of the intestinal barrier-protecting effect. Taken together, D-Ala in TA played an important role in the barrier-protecting effect of S. thermophilus in the intestinal epithelium, and these beneficial effects could be enhanced by exogenous L-Ala.

Original languageEnglish
Pages (from-to)283-288
Number of pages6
JournalBioscience, Biotechnology and Biochemistry
Volume76
Issue number2
DOIs
Publication statusPublished - 2012
Externally publishedYes

Fingerprint

Teichoic Acids
Streptococcus thermophilus
Alanine
Cell Wall
Epithelial Cells
Cells
Acoustic impedance
Electric Impedance
Caco-2 Cells
Gram-Positive Bacteria
Intestinal Mucosa
Bacteria
Tumor Necrosis Factor-alpha
Messenger RNA
Temperature
Growth

Keywords

  • Caco-2
  • D-alanine
  • Intestinal barrier
  • Streptococcus thermophilus
  • Teichoic acid

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Applied Microbiology and Biotechnology
  • Analytical Chemistry
  • Organic Chemistry

Cite this

Effect of D-alanine in teichoic acid from the Streptococcus thermophilus cell wall on the barrier-protection of intestinal epithelial cells. / Miyauchi, Eiji; Morita, Maho; Rossi, Mauro; Morita, Hidetoshi; Suzuki, Takuya; Tanabe, Soichi.

In: Bioscience, Biotechnology and Biochemistry, Vol. 76, No. 2, 2012, p. 283-288.

Research output: Contribution to journalArticle

@article{dfb314d2041340dd9cbf087cbc5037cd,
title = "Effect of D-alanine in teichoic acid from the Streptococcus thermophilus cell wall on the barrier-protection of intestinal epithelial cells",
abstract = "D-Alanylation of teichoic acid (TA) affects various functions of Gram-positive bacteria, including immunomodulatory effects. We investigated in this study the impact of D-alanine (D-Ala) in TA from Streptococcus thermophilus ATCC 19258 T on the barrier-protecting effect in human intestinal Caco-2 cells. ATCC 19258 T suppressed the tumor necrosis factor-α-induced decrease in transepithelial electrical resistance (TER), an indicator of the barrier function. The D-alanylation of TA in ATCC 19258 T was growth phase-and culture temperature-dependent. Treatment of ATCC 19258 T with Mg 2+ decreased the dlt mRNA expression and DAla content in TA and also abolished the suppressive effect on the TER decrease. Supplementation with Lalanine (L-Ala) to the broth led to an increase of D-Ala in ATCC 19258 T and of the intestinal barrier-protecting effect. Taken together, D-Ala in TA played an important role in the barrier-protecting effect of S. thermophilus in the intestinal epithelium, and these beneficial effects could be enhanced by exogenous L-Ala.",
keywords = "Caco-2, D-alanine, Intestinal barrier, Streptococcus thermophilus, Teichoic acid",
author = "Eiji Miyauchi and Maho Morita and Mauro Rossi and Hidetoshi Morita and Takuya Suzuki and Soichi Tanabe",
year = "2012",
doi = "10.1271/bbb.110646",
language = "English",
volume = "76",
pages = "283--288",
journal = "Bioscience, Biotechnology and Biochemistry",
issn = "0916-8451",
publisher = "Japan Society for Bioscience Biotechnology and Agrochemistry",
number = "2",

}

TY - JOUR

T1 - Effect of D-alanine in teichoic acid from the Streptococcus thermophilus cell wall on the barrier-protection of intestinal epithelial cells

AU - Miyauchi, Eiji

AU - Morita, Maho

AU - Rossi, Mauro

AU - Morita, Hidetoshi

AU - Suzuki, Takuya

AU - Tanabe, Soichi

PY - 2012

Y1 - 2012

N2 - D-Alanylation of teichoic acid (TA) affects various functions of Gram-positive bacteria, including immunomodulatory effects. We investigated in this study the impact of D-alanine (D-Ala) in TA from Streptococcus thermophilus ATCC 19258 T on the barrier-protecting effect in human intestinal Caco-2 cells. ATCC 19258 T suppressed the tumor necrosis factor-α-induced decrease in transepithelial electrical resistance (TER), an indicator of the barrier function. The D-alanylation of TA in ATCC 19258 T was growth phase-and culture temperature-dependent. Treatment of ATCC 19258 T with Mg 2+ decreased the dlt mRNA expression and DAla content in TA and also abolished the suppressive effect on the TER decrease. Supplementation with Lalanine (L-Ala) to the broth led to an increase of D-Ala in ATCC 19258 T and of the intestinal barrier-protecting effect. Taken together, D-Ala in TA played an important role in the barrier-protecting effect of S. thermophilus in the intestinal epithelium, and these beneficial effects could be enhanced by exogenous L-Ala.

AB - D-Alanylation of teichoic acid (TA) affects various functions of Gram-positive bacteria, including immunomodulatory effects. We investigated in this study the impact of D-alanine (D-Ala) in TA from Streptococcus thermophilus ATCC 19258 T on the barrier-protecting effect in human intestinal Caco-2 cells. ATCC 19258 T suppressed the tumor necrosis factor-α-induced decrease in transepithelial electrical resistance (TER), an indicator of the barrier function. The D-alanylation of TA in ATCC 19258 T was growth phase-and culture temperature-dependent. Treatment of ATCC 19258 T with Mg 2+ decreased the dlt mRNA expression and DAla content in TA and also abolished the suppressive effect on the TER decrease. Supplementation with Lalanine (L-Ala) to the broth led to an increase of D-Ala in ATCC 19258 T and of the intestinal barrier-protecting effect. Taken together, D-Ala in TA played an important role in the barrier-protecting effect of S. thermophilus in the intestinal epithelium, and these beneficial effects could be enhanced by exogenous L-Ala.

KW - Caco-2

KW - D-alanine

KW - Intestinal barrier

KW - Streptococcus thermophilus

KW - Teichoic acid

UR - http://www.scopus.com/inward/record.url?scp=84863296694&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84863296694&partnerID=8YFLogxK

U2 - 10.1271/bbb.110646

DO - 10.1271/bbb.110646

M3 - Article

C2 - 22313760

AN - SCOPUS:84863296694

VL - 76

SP - 283

EP - 288

JO - Bioscience, Biotechnology and Biochemistry

JF - Bioscience, Biotechnology and Biochemistry

SN - 0916-8451

IS - 2

ER -