EFFECT OF CYP2A6*4 GENETIC POLYMORPHISMS ON SMOKING BEHAVIORS AND NICOTINE DEPENDENCE IN A GENERAL POPULATION OF JAPANESE MEN

Teruna Ito, Masayoshi Tsuji, Yayoi Mori, Hideyuki Kanda, Tomoo Hidaka, Takeyasu Kakamu, Tomohiro Kumagai, Takehito Hayakawa, Yoneatsu Osaki, And Tetsuhito Fukushima

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

OBJECTIVES: Nicotine in cigarettes is metabolized primarily by CYP2A6-catalyzed oxidation. The CYP2A6*4 allele, in which CYP2A6 is a homozygous whole-deletion variant, completely lacks enzyme activity. The aim of this study was to examine the effects of CYP2A6*4 genetic polymorphism on smoking behavior and nicotine dependence in a general population of Japanese men.

METHODS: The subjects were 124 healthy Japanese men who gave informed consent to give saliva samples. The survey items included general information, smoking behaviors and nicotine dependence. The polymerase chain reaction restriction fragment length polymorphism method was used to analyze the genetic polymorphisms of CYP2A6. The subjects were classified into two groups: Group W (CYP2A6*4 absence: *1A/*1A, *1A/*1B and *1B/*1B) and Group D (CYP2A6*4 presence: *1B/*4A, *4A/*4A, *1A/*4A or *1B/*4D, and *1A/*4D). We analyzed the differences in the survey items between the two groups.

RESULTS: There were no significant differences in smoking behaviors between the two groups. However, Group D tended to have less difficulty in refraining from smoking after waking in the morning compared to Group W (p=0.051).

CONCLUSIONS: CYP2A6*4 genetic polymorphisms may not strongly affect smoking behavior but may possibly have an effect on nicotine dependence.

Original languageEnglish
Pages (from-to)125-130
Number of pages6
JournalFukushima journal of medical science
Volume61
Issue number2
DOIs
Publication statusPublished - Jan 1 2015
Externally publishedYes

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Tobacco Use Disorder
Genetic Polymorphisms
Smoking
Population
Informed Consent
Nicotine
Saliva
Tobacco Products
Restriction Fragment Length Polymorphisms
Alleles
Polymerase Chain Reaction
Enzymes

ASJC Scopus subject areas

  • Medicine(all)

Cite this

EFFECT OF CYP2A6*4 GENETIC POLYMORPHISMS ON SMOKING BEHAVIORS AND NICOTINE DEPENDENCE IN A GENERAL POPULATION OF JAPANESE MEN. / Ito, Teruna; Tsuji, Masayoshi; Mori, Yayoi; Kanda, Hideyuki; Hidaka, Tomoo; Kakamu, Takeyasu; Kumagai, Tomohiro; Hayakawa, Takehito; Osaki, Yoneatsu; Fukushima, And Tetsuhito.

In: Fukushima journal of medical science, Vol. 61, No. 2, 01.01.2015, p. 125-130.

Research output: Contribution to journalArticle

Ito, T, Tsuji, M, Mori, Y, Kanda, H, Hidaka, T, Kakamu, T, Kumagai, T, Hayakawa, T, Osaki, Y & Fukushima, AT 2015, 'EFFECT OF CYP2A6*4 GENETIC POLYMORPHISMS ON SMOKING BEHAVIORS AND NICOTINE DEPENDENCE IN A GENERAL POPULATION OF JAPANESE MEN', Fukushima journal of medical science, vol. 61, no. 2, pp. 125-130. https://doi.org/10.5387/fms.2015-14
Ito, Teruna ; Tsuji, Masayoshi ; Mori, Yayoi ; Kanda, Hideyuki ; Hidaka, Tomoo ; Kakamu, Takeyasu ; Kumagai, Tomohiro ; Hayakawa, Takehito ; Osaki, Yoneatsu ; Fukushima, And Tetsuhito. / EFFECT OF CYP2A6*4 GENETIC POLYMORPHISMS ON SMOKING BEHAVIORS AND NICOTINE DEPENDENCE IN A GENERAL POPULATION OF JAPANESE MEN. In: Fukushima journal of medical science. 2015 ; Vol. 61, No. 2. pp. 125-130.
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abstract = "OBJECTIVES: Nicotine in cigarettes is metabolized primarily by CYP2A6-catalyzed oxidation. The CYP2A6*4 allele, in which CYP2A6 is a homozygous whole-deletion variant, completely lacks enzyme activity. The aim of this study was to examine the effects of CYP2A6*4 genetic polymorphism on smoking behavior and nicotine dependence in a general population of Japanese men.METHODS: The subjects were 124 healthy Japanese men who gave informed consent to give saliva samples. The survey items included general information, smoking behaviors and nicotine dependence. The polymerase chain reaction restriction fragment length polymorphism method was used to analyze the genetic polymorphisms of CYP2A6. The subjects were classified into two groups: Group W (CYP2A6*4 absence: *1A/*1A, *1A/*1B and *1B/*1B) and Group D (CYP2A6*4 presence: *1B/*4A, *4A/*4A, *1A/*4A or *1B/*4D, and *1A/*4D). We analyzed the differences in the survey items between the two groups.RESULTS: There were no significant differences in smoking behaviors between the two groups. However, Group D tended to have less difficulty in refraining from smoking after waking in the morning compared to Group W (p=0.051).CONCLUSIONS: CYP2A6*4 genetic polymorphisms may not strongly affect smoking behavior but may possibly have an effect on nicotine dependence.",
author = "Teruna Ito and Masayoshi Tsuji and Yayoi Mori and Hideyuki Kanda and Tomoo Hidaka and Takeyasu Kakamu and Tomohiro Kumagai and Takehito Hayakawa and Yoneatsu Osaki and Fukushima, {And Tetsuhito}",
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AU - Ito, Teruna

AU - Tsuji, Masayoshi

AU - Mori, Yayoi

AU - Kanda, Hideyuki

AU - Hidaka, Tomoo

AU - Kakamu, Takeyasu

AU - Kumagai, Tomohiro

AU - Hayakawa, Takehito

AU - Osaki, Yoneatsu

AU - Fukushima, And Tetsuhito

PY - 2015/1/1

Y1 - 2015/1/1

N2 - OBJECTIVES: Nicotine in cigarettes is metabolized primarily by CYP2A6-catalyzed oxidation. The CYP2A6*4 allele, in which CYP2A6 is a homozygous whole-deletion variant, completely lacks enzyme activity. The aim of this study was to examine the effects of CYP2A6*4 genetic polymorphism on smoking behavior and nicotine dependence in a general population of Japanese men.METHODS: The subjects were 124 healthy Japanese men who gave informed consent to give saliva samples. The survey items included general information, smoking behaviors and nicotine dependence. The polymerase chain reaction restriction fragment length polymorphism method was used to analyze the genetic polymorphisms of CYP2A6. The subjects were classified into two groups: Group W (CYP2A6*4 absence: *1A/*1A, *1A/*1B and *1B/*1B) and Group D (CYP2A6*4 presence: *1B/*4A, *4A/*4A, *1A/*4A or *1B/*4D, and *1A/*4D). We analyzed the differences in the survey items between the two groups.RESULTS: There were no significant differences in smoking behaviors between the two groups. However, Group D tended to have less difficulty in refraining from smoking after waking in the morning compared to Group W (p=0.051).CONCLUSIONS: CYP2A6*4 genetic polymorphisms may not strongly affect smoking behavior but may possibly have an effect on nicotine dependence.

AB - OBJECTIVES: Nicotine in cigarettes is metabolized primarily by CYP2A6-catalyzed oxidation. The CYP2A6*4 allele, in which CYP2A6 is a homozygous whole-deletion variant, completely lacks enzyme activity. The aim of this study was to examine the effects of CYP2A6*4 genetic polymorphism on smoking behavior and nicotine dependence in a general population of Japanese men.METHODS: The subjects were 124 healthy Japanese men who gave informed consent to give saliva samples. The survey items included general information, smoking behaviors and nicotine dependence. The polymerase chain reaction restriction fragment length polymorphism method was used to analyze the genetic polymorphisms of CYP2A6. The subjects were classified into two groups: Group W (CYP2A6*4 absence: *1A/*1A, *1A/*1B and *1B/*1B) and Group D (CYP2A6*4 presence: *1B/*4A, *4A/*4A, *1A/*4A or *1B/*4D, and *1A/*4D). We analyzed the differences in the survey items between the two groups.RESULTS: There were no significant differences in smoking behaviors between the two groups. However, Group D tended to have less difficulty in refraining from smoking after waking in the morning compared to Group W (p=0.051).CONCLUSIONS: CYP2A6*4 genetic polymorphisms may not strongly affect smoking behavior but may possibly have an effect on nicotine dependence.

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