Effect of cobalamin on the allergic response in mice

Ulala Funada, Masahiro Wada, Tetsunori Kawata, Nobuo Tanaka, Tadahiro Tadokoro, Akio Maekawa

Research output: Contribution to journalArticlepeer-review


Cyanocobalamin, deoxyadenosylcobalamin and methylcobalamin were separately administered to mice sensitized with the ovalbumin antigen to investigate the allergic response. The serum IgE and pulmonary histamine concentrations were significantly lower in all cobalamin (Cbl)-administered groups. The production of interleukin (IL)-2 and IL-4 in splenocytes was also lower in all Cbl-administered groups. These results show that Cbls were effective in mitigating allergic reactions and IL-2 production. CD3+CD28+ (CD28 is an accessory molecule related to IL-2 production) and CD4+CD28+ in splenocytes were higher in all the Cbl-administered groups. However, CD3+CD28, CD4+CD28 and CD5+CD25 (CD25: IL-2 Rα/p55) were lower in the Cbl-administered groups. In addition, Cbl specifically inhibited the cellular phosphorylation of tyrosine induced by ovalbumin sensitization. These results indicate that the signal in a cell by CD 28 was restrained by Cbl. We infer that Cbl administration significantly reduced the IL-2 concentration, and secondarily the IL-4, IgE and histamine concentrations.

Original languageEnglish
Pages (from-to)2053-2058
Number of pages6
JournalBioscience, Biotechnology and Biochemistry
Issue number10
Publication statusPublished - 2000


  • Allergy
  • Cobalamin
  • IL-2
  • IL-4
  • IgE

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry


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