Effect of calcium ion concentrations on osteogenic differentiation and hematopoietic stem cell niche-related protein expression in osteoblasts

Shinya Nakamura, Takuya Matsumoto, Jun Ichi Sasaki, Hiroshi Egusa, Kuen Yong Lee, Takayoshi Nakano, Taiji Sohmura, Atsushi Nakahira

Research output: Contribution to journalArticle

82 Citations (Scopus)

Abstract

Apatite biomaterials have potential not only as cell vehicles for engineering bone tissue but also as regulators of calcium (Ca) concentration in situ for controlling osteoblast functions, for example, osteogenic differentiation and fate management of hematopoietic stem cells (HSCs). To design apatite materials having optimal chemical properties for the latter purpose, more detailed investigations into what effect Ca concentrations have on osteoblast functions is crucial. In this study, osteoblasts were cultured at different Ca concentrations, and the temporal alterations in osteogenic differentiation and HSC niche-related protein (angiopoietin-1, 2 [Ang1, 2]) expression were investigated. The different Ca concentrations (1.8-50mmol/L) in the cell culture medium had no effect on the proliferation of osteoblasts, but did on the cell morphology. The higher Ca concentrations (

Original languageEnglish
Pages (from-to)2467-2473
Number of pages7
JournalTissue Engineering - Part A
Volume16
Issue number8
DOIs
Publication statusPublished - Aug 1 2010
Externally publishedYes

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Stem Cell Niche
Osteoblasts
Hematopoietic Stem Cells
Stem cells
Calcium
Ions
Proteins
Apatites
Apatite
Angiopoietin-2
Angiopoietin-1
Cell Engineering
Biocompatible Materials
Cell culture
Biomaterials
Chemical properties
Culture Media
Bone
Cell Culture Techniques
Tissue

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Biomedical Engineering
  • Biomaterials
  • Medicine(all)

Cite this

Effect of calcium ion concentrations on osteogenic differentiation and hematopoietic stem cell niche-related protein expression in osteoblasts. / Nakamura, Shinya; Matsumoto, Takuya; Sasaki, Jun Ichi; Egusa, Hiroshi; Lee, Kuen Yong; Nakano, Takayoshi; Sohmura, Taiji; Nakahira, Atsushi.

In: Tissue Engineering - Part A, Vol. 16, No. 8, 01.08.2010, p. 2467-2473.

Research output: Contribution to journalArticle

Nakamura, Shinya ; Matsumoto, Takuya ; Sasaki, Jun Ichi ; Egusa, Hiroshi ; Lee, Kuen Yong ; Nakano, Takayoshi ; Sohmura, Taiji ; Nakahira, Atsushi. / Effect of calcium ion concentrations on osteogenic differentiation and hematopoietic stem cell niche-related protein expression in osteoblasts. In: Tissue Engineering - Part A. 2010 ; Vol. 16, No. 8. pp. 2467-2473.
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