Effect of Brn-3a deficiency on primary nociceptors in the trigeminal ganglion

H. Ichikawa, S. Schulz, V. Höllt, Z. Mo, M. Xiang, T. Sugimoto

Research output: Contribution to journalArticle

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Abstract

Immunohistochemistry for substance P, somatostatin and vanilloid receptor subtype 1 as well as receptors for somatostatin and opioids was performed on the trigeminal ganglion in wild-type and Brn-3a knockout mice at postnatal day 0. In wild-type mice, the trigeminal ganglion contained abundant substance P-, vanilloid receptor subtype 1-, sst2A receptor- and delta-opioid receptor-immunoreactive neurons, while the ganglion had only a few mu-opioid receptor-immunoreactive neurons. The Brn-3a deficiency had an effect on the cell size but not the number of substance P-immunoreactive neurons. In knockout mice, the proportion of small immunoreactive neurons markedly increased and that of medium- to large-sized immunoreactive ones correspondingly decreased (mean ± S.D. = 54.7 ± 29.1 μm2, range = 10.9-220.8 μm2) compared to wild-type mice (mean ± S.D. = 116.6 ± 58.6 μm2, range = 27.3-400.7 μm2). As for vanilloid receptor subtype 1-immunoreactive neurons, the number and cell size was barely affected by the deficiency. On the other hand, the loss of Brn-3a caused a decrease in the number of sst2A receptor- or delta-opioid receptor-immunoreactive neurons (more than 95% reduction) and an increase in the number of mu-opioid receptor-immunoreactive neurons (9.3-fold increase). Somatostatin-immunoreactive neurons were not detected in the trigeminal ganglion of wild-type or mutant mice at postnatal day 0. The present study suggests that Brn-3a deficiency may have effects on the survival of trigeminal nociceptors and their expression of some neurochemical substances.

Original languageEnglish
Pages (from-to)445-451
Number of pages7
JournalNeuroscience Research
Volume51
Issue number4
DOIs
Publication statusPublished - Apr 2005

Fingerprint

Trigeminal Ganglion
Nociceptors
Neurons
Neurokinin-1 Receptors
Somatostatin Receptors
delta Opioid Receptor
mu Opioid Receptor
Cell Size
Knockout Mice
Opioid Receptors
Substance P
Somatostatin
Ganglia
Immunohistochemistry

Keywords

  • Capsaicin receptor
  • Immunohistochemistry
  • Knockout mouse
  • Neuropeptides
  • Transcription factor
  • Trigeminal ganglion

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Ichikawa, H., Schulz, S., Höllt, V., Mo, Z., Xiang, M., & Sugimoto, T. (2005). Effect of Brn-3a deficiency on primary nociceptors in the trigeminal ganglion. Neuroscience Research, 51(4), 445-451. https://doi.org/10.1016/j.neures.2004.12.014

Effect of Brn-3a deficiency on primary nociceptors in the trigeminal ganglion. / Ichikawa, H.; Schulz, S.; Höllt, V.; Mo, Z.; Xiang, M.; Sugimoto, T.

In: Neuroscience Research, Vol. 51, No. 4, 04.2005, p. 445-451.

Research output: Contribution to journalArticle

Ichikawa, H, Schulz, S, Höllt, V, Mo, Z, Xiang, M & Sugimoto, T 2005, 'Effect of Brn-3a deficiency on primary nociceptors in the trigeminal ganglion', Neuroscience Research, vol. 51, no. 4, pp. 445-451. https://doi.org/10.1016/j.neures.2004.12.014
Ichikawa, H. ; Schulz, S. ; Höllt, V. ; Mo, Z. ; Xiang, M. ; Sugimoto, T. / Effect of Brn-3a deficiency on primary nociceptors in the trigeminal ganglion. In: Neuroscience Research. 2005 ; Vol. 51, No. 4. pp. 445-451.
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