Effect of biological response modifiers against pulmonary candidiasis in neutropenic mice

M. Futenma, F. Higa, K. X. Zhang, Nobuchika Kusano, A. Saito

Research output: Contribution to journalArticle

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Abstract

We investigated the prophylactic and therapeutic effects of biological response modifiers (rHG-CSF, M-CSF, rhIL-2) on pulmonary candidiasis in neutropenic mice. Cyclophosphamide treated mice were injected by the intratracheal route with 5 x 10(6) Candida yeast cells. Prophylactic treatment with rhG-CSF afforded significant protection against pulmonary candidiasis in neutropenic mice. Treatment with rhG-CSF also increased the number of peripheral blood neutrophils. The histopathological investigations in our experiments showed that the assembly of PMNs to the infected lung at 24 hrs after bacterial challenge was more remarkable in the rhG-CSF treated mice than that in the vehicle alone. Number of viable candida cells in the infected lung in the rhG-CSF treated mice were significantly decreased. The combination of rhG-CSF and fluconazole was more effective than those of each monotherapy. Prophylactic treatment with M-CSF or rhIL-2 had no influence on pulmonary candidiasis. These results show the possibility that rhG-CSF could be of help for treating human deep candidiasis not successfully treated with antimicrobial agents alone.

Original languageEnglish
Pages (from-to)513-519
Number of pages7
JournalKansenshogaku zasshi. The Journal of the Japanese Association for Infectious Diseases
Volume68
Issue number4
Publication statusPublished - Apr 1994
Externally publishedYes

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Candidiasis
Immunologic Factors
Macrophage Colony-Stimulating Factor
Lung
Candida
Fluconazole
Therapeutic Uses
Granulocyte-Macrophage Colony-Stimulating Factor
Anti-Infective Agents
Cyclophosphamide
Neutrophils
Yeasts

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Effect of biological response modifiers against pulmonary candidiasis in neutropenic mice. / Futenma, M.; Higa, F.; Zhang, K. X.; Kusano, Nobuchika; Saito, A.

In: Kansenshogaku zasshi. The Journal of the Japanese Association for Infectious Diseases, Vol. 68, No. 4, 04.1994, p. 513-519.

Research output: Contribution to journalArticle

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