Abstract
Removal of bicarbonate from spinach photosystem II BBY particles by means of washing in a CO2-free medium results in the loss of their capability to accumulate the S2 multiline EPR signal upon continuous illumination at 190 K. Addition of 1 mM NaHCO3 before illumination leads to a 50-60% restoration of the multiline signal. Similarly, in BBY particles depleted of Mn by treatment with 1 mM Tris-HCl (pH 8.0) and 0.5 M MgCl2, re-addition of MnCl2 in the presence of 1 mM NaHCO3 results in a partial restoration (~ 30%) of the S2 multiline EPR signal of the Mn cluster, while in the absence of NaHCO3 no restoration is observed. The results provide further evidence that bicarbonate is essential for maintaining the Mn-containing oxygen-evolving complex of PS II in a functionally active form.
| Original language | English |
|---|---|
| Pages (from-to) | 146-148 |
| Number of pages | 3 |
| Journal | FEBS Letters |
| Volume | 424 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Mar 13 1998 |
| Externally published | Yes |
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Keywords
- Bicarbonate
- Donor side
- Manganese
- Multiline EPR signal
- Photosystem II
- S state
ASJC Scopus subject areas
- Biophysics
- Structural Biology
- Biochemistry
- Molecular Biology
- Genetics
- Cell Biology
Cite this
Effect of bicarbonate on the S2 multiline EPR signal of the oxygen-evolving complex in photosystem II membrane fragments. / Hulsebosch, R. J.; Allakhverdiev, Suleyman; Klimov, V. V.; Picorel, R.; Hoff, A. J.
In: FEBS Letters, Vol. 424, No. 3, 13.03.1998, p. 146-148.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Effect of bicarbonate on the S2 multiline EPR signal of the oxygen-evolving complex in photosystem II membrane fragments
AU - Hulsebosch, R. J.
AU - Allakhverdiev, Suleyman
AU - Klimov, V. V.
AU - Picorel, R.
AU - Hoff, A. J.
PY - 1998/3/13
Y1 - 1998/3/13
N2 - Removal of bicarbonate from spinach photosystem II BBY particles by means of washing in a CO2-free medium results in the loss of their capability to accumulate the S2 multiline EPR signal upon continuous illumination at 190 K. Addition of 1 mM NaHCO3 before illumination leads to a 50-60% restoration of the multiline signal. Similarly, in BBY particles depleted of Mn by treatment with 1 mM Tris-HCl (pH 8.0) and 0.5 M MgCl2, re-addition of MnCl2 in the presence of 1 mM NaHCO3 results in a partial restoration (~ 30%) of the S2 multiline EPR signal of the Mn cluster, while in the absence of NaHCO3 no restoration is observed. The results provide further evidence that bicarbonate is essential for maintaining the Mn-containing oxygen-evolving complex of PS II in a functionally active form.
AB - Removal of bicarbonate from spinach photosystem II BBY particles by means of washing in a CO2-free medium results in the loss of their capability to accumulate the S2 multiline EPR signal upon continuous illumination at 190 K. Addition of 1 mM NaHCO3 before illumination leads to a 50-60% restoration of the multiline signal. Similarly, in BBY particles depleted of Mn by treatment with 1 mM Tris-HCl (pH 8.0) and 0.5 M MgCl2, re-addition of MnCl2 in the presence of 1 mM NaHCO3 results in a partial restoration (~ 30%) of the S2 multiline EPR signal of the Mn cluster, while in the absence of NaHCO3 no restoration is observed. The results provide further evidence that bicarbonate is essential for maintaining the Mn-containing oxygen-evolving complex of PS II in a functionally active form.
KW - Bicarbonate
KW - Donor side
KW - Manganese
KW - Multiline EPR signal
KW - Photosystem II
KW - S state
UR - http://www.scopus.com/inward/record.url?scp=0032512934&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032512934&partnerID=8YFLogxK
U2 - 10.1016/S0014-5793(98)00163-X
DO - 10.1016/S0014-5793(98)00163-X
M3 - Article
C2 - 9539139
AN - SCOPUS:0032512934
VL - 424
SP - 146
EP - 148
JO - FEBS Letters
JF - FEBS Letters
SN - 0014-5793
IS - 3
ER -