Effect of bicarbonate on the S2 multiline EPR signal of the oxygen-evolving complex in photosystem II membrane fragments

R. J. Hulsebosch, Suleyman Allakhverdiev, V. V. Klimov, R. Picorel, A. J. Hoff

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Removal of bicarbonate from spinach photosystem II BBY particles by means of washing in a CO2-free medium results in the loss of their capability to accumulate the S2 multiline EPR signal upon continuous illumination at 190 K. Addition of 1 mM NaHCO3 before illumination leads to a 50-60% restoration of the multiline signal. Similarly, in BBY particles depleted of Mn by treatment with 1 mM Tris-HCl (pH 8.0) and 0.5 M MgCl2, re-addition of MnCl2 in the presence of 1 mM NaHCO3 results in a partial restoration (~ 30%) of the S2 multiline EPR signal of the Mn cluster, while in the absence of NaHCO3 no restoration is observed. The results provide further evidence that bicarbonate is essential for maintaining the Mn-containing oxygen-evolving complex of PS II in a functionally active form.

Original languageEnglish
Pages (from-to)146-148
Number of pages3
JournalFEBS Letters
Volume424
Issue number3
DOIs
Publication statusPublished - Mar 13 1998
Externally publishedYes

Fingerprint

Photosystem II Protein Complex
Bicarbonates
Lighting
Restoration
Paramagnetic resonance
Oxygen
Membranes
Magnesium Chloride
Spinacia oleracea
Washing
manganese chloride

Keywords

  • Bicarbonate
  • Donor side
  • Manganese
  • Multiline EPR signal
  • Photosystem II
  • S state

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Effect of bicarbonate on the S2 multiline EPR signal of the oxygen-evolving complex in photosystem II membrane fragments. / Hulsebosch, R. J.; Allakhverdiev, Suleyman; Klimov, V. V.; Picorel, R.; Hoff, A. J.

In: FEBS Letters, Vol. 424, No. 3, 13.03.1998, p. 146-148.

Research output: Contribution to journalArticle

Hulsebosch, R. J. ; Allakhverdiev, Suleyman ; Klimov, V. V. ; Picorel, R. ; Hoff, A. J. / Effect of bicarbonate on the S2 multiline EPR signal of the oxygen-evolving complex in photosystem II membrane fragments. In: FEBS Letters. 1998 ; Vol. 424, No. 3. pp. 146-148.
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AU - Picorel, R.

AU - Hoff, A. J.

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