Effect of bicarbonate on the S2 multiline EPR signal of the oxygen-evolving complex in photosystem II membrane fragments

R. J. Hulsebosch; S. I. Allakhverdiev; V. V. Klimov; R. Picorel; A. J. Hoff

doi:10.1016/S0014-5793(98)00163-X

Effect of bicarbonate on the S₂ multiline EPR signal of the oxygen-evolving complex in photosystem II membrane fragments

R. J. Hulsebosch, S. I. Allakhverdiev, V. V. Klimov, R. Picorel, A. J. Hoff

Research Institute for Interdisciplinary Science

Research output: Contribution to journal › Article › peer-review

13 Citations (Scopus)

Abstract

Removal of bicarbonate from spinach photosystem II BBY particles by means of washing in a CO₂-free medium results in the loss of their capability to accumulate the S₂ multiline EPR signal upon continuous illumination at 190 K. Addition of 1 mM NaHCO₃ before illumination leads to a 50-60% restoration of the multiline signal. Similarly, in BBY particles depleted of Mn by treatment with 1 mM Tris-HCl (pH 8.0) and 0.5 M MgCl₂, re-addition of MnCl₂ in the presence of 1 mM NaHCO₃ results in a partial restoration (~ 30%) of the S₂ multiline EPR signal of the Mn cluster, while in the absence of NaHCO₃ no restoration is observed. The results provide further evidence that bicarbonate is essential for maintaining the Mn-containing oxygen-evolving complex of PS II in a functionally active form.

Original language	English
Pages (from-to)	146-148
Number of pages	3
Journal	FEBS Letters
Volume	424
Issue number	3
DOIs	https://doi.org/10.1016/S0014-5793(98)00163-X
Publication status	Published - Mar 13 1998

Keywords

Bicarbonate
Donor side
Manganese
Multiline EPR signal
Photosystem II
S state

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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title = "Effect of bicarbonate on the S2 multiline EPR signal of the oxygen-evolving complex in photosystem II membrane fragments",

abstract = "Removal of bicarbonate from spinach photosystem II BBY particles by means of washing in a CO2-free medium results in the loss of their capability to accumulate the S2 multiline EPR signal upon continuous illumination at 190 K. Addition of 1 mM NaHCO3 before illumination leads to a 50-60% restoration of the multiline signal. Similarly, in BBY particles depleted of Mn by treatment with 1 mM Tris-HCl (pH 8.0) and 0.5 M MgCl2, re-addition of MnCl2 in the presence of 1 mM NaHCO3 results in a partial restoration (~ 30%) of the S2 multiline EPR signal of the Mn cluster, while in the absence of NaHCO3 no restoration is observed. The results provide further evidence that bicarbonate is essential for maintaining the Mn-containing oxygen-evolving complex of PS II in a functionally active form.",

keywords = "Bicarbonate, Donor side, Manganese, Multiline EPR signal, Photosystem II, S state",

author = "Hulsebosch, {R. J.} and Allakhverdiev, {S. I.} and Klimov, {V. V.} and R. Picorel and Hoff, {A. J.}",

note = "Funding Information: This work was supported by the Life Sciences Foundation (SLW), and financed by the Netherlands Organisation for Scientific Research (NWO), the INTAS foundation (Grant 93-2849-Ext), the Russian Foundation for Basic Research (Grant 96-04-50394), and the DGICYT from Spain (Grant PB95-0219). ",

year = "1998",

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doi = "10.1016/S0014-5793(98)00163-X",

language = "English",

volume = "424",

pages = "146--148",

journal = "FEBS Letters",

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T1 - Effect of bicarbonate on the S2 multiline EPR signal of the oxygen-evolving complex in photosystem II membrane fragments

AU - Hulsebosch, R. J.

AU - Allakhverdiev, S. I.

AU - Klimov, V. V.

AU - Picorel, R.

AU - Hoff, A. J.

N1 - Funding Information: This work was supported by the Life Sciences Foundation (SLW), and financed by the Netherlands Organisation for Scientific Research (NWO), the INTAS foundation (Grant 93-2849-Ext), the Russian Foundation for Basic Research (Grant 96-04-50394), and the DGICYT from Spain (Grant PB95-0219).

PY - 1998/3/13

Y1 - 1998/3/13

N2 - Removal of bicarbonate from spinach photosystem II BBY particles by means of washing in a CO2-free medium results in the loss of their capability to accumulate the S2 multiline EPR signal upon continuous illumination at 190 K. Addition of 1 mM NaHCO3 before illumination leads to a 50-60% restoration of the multiline signal. Similarly, in BBY particles depleted of Mn by treatment with 1 mM Tris-HCl (pH 8.0) and 0.5 M MgCl2, re-addition of MnCl2 in the presence of 1 mM NaHCO3 results in a partial restoration (~ 30%) of the S2 multiline EPR signal of the Mn cluster, while in the absence of NaHCO3 no restoration is observed. The results provide further evidence that bicarbonate is essential for maintaining the Mn-containing oxygen-evolving complex of PS II in a functionally active form.

AB - Removal of bicarbonate from spinach photosystem II BBY particles by means of washing in a CO2-free medium results in the loss of their capability to accumulate the S2 multiline EPR signal upon continuous illumination at 190 K. Addition of 1 mM NaHCO3 before illumination leads to a 50-60% restoration of the multiline signal. Similarly, in BBY particles depleted of Mn by treatment with 1 mM Tris-HCl (pH 8.0) and 0.5 M MgCl2, re-addition of MnCl2 in the presence of 1 mM NaHCO3 results in a partial restoration (~ 30%) of the S2 multiline EPR signal of the Mn cluster, while in the absence of NaHCO3 no restoration is observed. The results provide further evidence that bicarbonate is essential for maintaining the Mn-containing oxygen-evolving complex of PS II in a functionally active form.

KW - Bicarbonate

KW - Donor side

KW - Manganese

KW - Multiline EPR signal

KW - Photosystem II

KW - S state

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