Effect of ATP concentration and temperature on firefly luciferase activity

Ivan Galis, J. Jirásková

Research output: Contribution to journal › Article

Abstract

The dependence of luciferase activity in the homogenate of leaves of transgenic tobacco plants with chimeric firefly luciferase gene on ATP concentration and temperature was studied. The optimum ATP concentration was between 0.625 mM and 2.5 mM. The activity rapidly decreased if the homogenate was kept in 25°C and is completely lost during 30 min.

Original languageEnglish
Pages (from-to)147-150
Number of pages4
JournalBiologia Plantarum
Volume35
Issue number1
DOIs
Publication statusPublished - Mar 1993
Externally publishedYes

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luciferase
temperature
tobacco
genetically modified organisms
leaves
genes

ASJC Scopus subject areas

  • Plant Science

Cite this

Effect of ATP concentration and temperature on firefly luciferase activity. / Galis, Ivan; Jirásková, J.

In: Biologia Plantarum, Vol. 35, No. 1, 03.1993, p. 147-150.

Research output: Contribution to journal › Article

Galis, I & Jirásková, J 1993, 'Effect of ATP concentration and temperature on firefly luciferase activity', Biologia Plantarum, vol. 35, no. 1, pp. 147-150. https://doi.org/10.1007/BF02921140
Galis I, Jirásková J. Effect of ATP concentration and temperature on firefly luciferase activity. Biologia Plantarum. 1993 Mar;35(1):147-150. https://doi.org/10.1007/BF02921140
Galis, Ivan ; Jirásková, J. / Effect of ATP concentration and temperature on firefly luciferase activity. In: Biologia Plantarum. 1993 ; Vol. 35, No. 1. pp. 147-150.
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