Effect of ATP concentration and temperature on firefly luciferase activity

I. Gális; J. Jirásková

doi:10.1007/BF02921140

Effect of ATP concentration and temperature on firefly luciferase activity

I. Gális, J. Jirásková

Research output: Contribution to journal › Article › peer-review

Abstract

The dependence of luciferase activity in the homogenate of leaves of transgenic tobacco plants with chimeric firefly luciferase gene on ATP concentration and temperature was studied. The optimum ATP concentration was between 0.625 mM and 2.5 mM. The activity rapidly decreased if the homogenate was kept in 25°C and is completely lost during 30 min.

Original language	English
Pages (from-to)	147-150
Number of pages	4
Journal	Biologia Plantarum
Volume	35
Issue number	1
DOIs	https://doi.org/10.1007/BF02921140
Publication status	Published - Mar 1 1993
Externally published	Yes

ASJC Scopus subject areas

Plant Science
Horticulture

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10.1007/BF02921140

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title = "Effect of ATP concentration and temperature on firefly luciferase activity",

abstract = "The dependence of luciferase activity in the homogenate of leaves of transgenic tobacco plants with chimeric firefly luciferase gene on ATP concentration and temperature was studied. The optimum ATP concentration was between 0.625 mM and 2.5 mM. The activity rapidly decreased if the homogenate was kept in 25°C and is completely lost during 30 min.",

author = "I. G{\'a}lis and J. Jir{\'a}skov{\'a}",

year = "1993",

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doi = "10.1007/BF02921140",

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AU - Jirásková, J.

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N2 - The dependence of luciferase activity in the homogenate of leaves of transgenic tobacco plants with chimeric firefly luciferase gene on ATP concentration and temperature was studied. The optimum ATP concentration was between 0.625 mM and 2.5 mM. The activity rapidly decreased if the homogenate was kept in 25°C and is completely lost during 30 min.

AB - The dependence of luciferase activity in the homogenate of leaves of transgenic tobacco plants with chimeric firefly luciferase gene on ATP concentration and temperature was studied. The optimum ATP concentration was between 0.625 mM and 2.5 mM. The activity rapidly decreased if the homogenate was kept in 25°C and is completely lost during 30 min.

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JO - Biologia Plantarum

JF - Biologia Plantarum

SN - 0006-3134

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Effect of ATP concentration and temperature on firefly luciferase activity

Abstract

ASJC Scopus subject areas

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