Effect of ATP concentration and temperature on firefly luciferase activity

I. Gális, J. Jirásková

Research output: Contribution to journalArticle

Abstract

The dependence of luciferase activity in the homogenate of leaves of transgenic tobacco plants with chimeric firefly luciferase gene on ATP concentration and temperature was studied. The optimum ATP concentration was between 0.625 mM and 2.5 mM. The activity rapidly decreased if the homogenate was kept in 25°C and is completely lost during 30 min.

Original languageEnglish
Pages (from-to)147-150
Number of pages4
JournalBiologia Plantarum
Volume35
Issue number1
DOIs
Publication statusPublished - Mar 1 1993
Externally publishedYes

ASJC Scopus subject areas

  • Plant Science
  • Horticulture

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