Effect of ATP concentration and temperature on firefly luciferase activity

Ivan Galis, J. Jirásková

Research output: Contribution to journalArticle


The dependence of luciferase activity in the homogenate of leaves of transgenic tobacco plants with chimeric firefly luciferase gene on ATP concentration and temperature was studied. The optimum ATP concentration was between 0.625 mM and 2.5 mM. The activity rapidly decreased if the homogenate was kept in 25°C and is completely lost during 30 min.

Original languageEnglish
Pages (from-to)147-150
Number of pages4
JournalBiologia Plantarum
Issue number1
Publication statusPublished - Mar 1993
Externally publishedYes


ASJC Scopus subject areas

  • Plant Science

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