Effect of alterations of basic amino acid residues of Escherichia coli heat-stable enterotoxin II on enterotoxicity

Y. Fujii, Y. Okamuro, S. Hitotsubashi, A. Saito, N. Akashi, Keinosuke Okamoto

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Escherichia coli heat-stable enterotoxin II (STII) is composed of 48 amino acid residues. Among these, one histidine, two arginine, and six lysine residues are basic. Isoelectric focusing showed that the isoelectric point of STII is 9.7, indicating that the side chains of some of these basic amino acid residues project outside the molecule. To understand the role that these basic amino acid residues play in toxicity, STII was chemically modified with ethoxyformic anhydride, maleic anhydride, and phenylglyoxal, which alter the side chains of basic amino acid residues in proteins. Maleic anhydride, which modifies the ε amino group, caused a significant loss of enterotoxic activity, but the other two modifiers did not. This indicated that lysine residues play an important role in the expression of the enterotoxic activity of STII and that the contribution of the other basic amino acid residues to the toxicity is relatively low. To confirm this hypothesis, we substituted these nine basic amino acid residues by oligonucleotide-directed site- specific mutagenesis and examined the enterotoxicity of these purified mutant STIIs. The enterotoxic activity was reduced when the lysine residues at positions 18, 22, 23, and 46 were substituted. In particular, the substitution at positions 22 and 23 induced a remarkable reduction. These results demonstrate that the lysine residues at positions 22 and 23 are very important in the expression of the enterotoxic activity of STII.

Original languageEnglish
Pages (from-to)2295-2301
Number of pages7
JournalInfection and Immunity
Volume62
Issue number6
Publication statusPublished - 1994
Externally publishedYes

Fingerprint

Basic Amino Acids
Enterotoxins
Lysine
Escherichia coli
Maleic Anhydrides
Phenylglyoxal
Diethyl Pyrocarbonate
Isoelectric Point
Isoelectric Focusing
Site-Directed Mutagenesis
Histidine
Oligonucleotides
Arginine
heat stable toxin (E coli)
Amino Acids
Proteins

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Immunology
  • Infectious Diseases

Cite this

Effect of alterations of basic amino acid residues of Escherichia coli heat-stable enterotoxin II on enterotoxicity. / Fujii, Y.; Okamuro, Y.; Hitotsubashi, S.; Saito, A.; Akashi, N.; Okamoto, Keinosuke.

In: Infection and Immunity, Vol. 62, No. 6, 1994, p. 2295-2301.

Research output: Contribution to journalArticle

Fujii, Y, Okamuro, Y, Hitotsubashi, S, Saito, A, Akashi, N & Okamoto, K 1994, 'Effect of alterations of basic amino acid residues of Escherichia coli heat-stable enterotoxin II on enterotoxicity', Infection and Immunity, vol. 62, no. 6, pp. 2295-2301.
Fujii, Y. ; Okamuro, Y. ; Hitotsubashi, S. ; Saito, A. ; Akashi, N. ; Okamoto, Keinosuke. / Effect of alterations of basic amino acid residues of Escherichia coli heat-stable enterotoxin II on enterotoxicity. In: Infection and Immunity. 1994 ; Vol. 62, No. 6. pp. 2295-2301.
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