Effect of Al on the phytosiderophore-mediated solubilization of Fe and uptake of Fe-phytosiderophore complex in wheat (Triticum aestivum)

Yi Chieh Chang, Jian Feng Ma, Takashi Iwashita, Hideaki Matsumoto

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Abstract

The effect of Aluminum (Al) on phytosiderophore-mediated solubilization of insoluble Fe and the uptake of phytosiderophore-Fe3+ complex was examined in wheat (Triticum aestivum L. cv. Atlas 66). Al addition did not affect the Fe solubilization by 2'-deoxymugineic acid (DMA), although Cu addition significantly inhibited the solubilization capacity. Addition of ten times more Al than Fe to the solution of DMA-Fe3+ complex did not decrease the absorption of the DMA-Fe3+ complex at 375 nm. Furthermore, NMR study indicated that Al did not shift the proton chemical shifts of DMA. All these results suggest that Al could not form a complex with the phytosiderophore, and is thereby unlikely to affect the process of phytosiderophore-mediated solubilization of Fe. Exposure of root to Al up to 100 μM for 3 h did not inhibit the DMA-Fe3+ uptake by the roots, but longer pretreatment (> 6 h) inhibited the uptake of the DMA-Fe3+ by more than 50%. Neither the uptake of DMA-Fe3+ nor root elongation was inhibited by 24 h pretreatment with 10 μM Al, but both uptake and root elongation were inhibited by higher Al (> 20 μM) pretreatment. These results suggest that Al did not directly block the transport of the phytosiderophore-Fe3+ complex, and that the decreased uptake of the phytosiderophore-Fe3+ complex resulted from the roots being damaged by Al.

Original languageEnglish
Pages (from-to)62-68
Number of pages7
JournalPhysiologia Plantarum
Volume106
Issue number1
DOIs
Publication statusPublished - May 1999
Externally publishedYes

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phytosiderophores
Aluminum
solubilization
Triticum
aluminum
Triticum aestivum
uptake mechanisms
wheat
acids
Acids
pretreatment
root growth
Atlases
protons
Protons

ASJC Scopus subject areas

  • Plant Science

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Effect of Al on the phytosiderophore-mediated solubilization of Fe and uptake of Fe-phytosiderophore complex in wheat (Triticum aestivum). / Chang, Yi Chieh; Ma, Jian Feng; Iwashita, Takashi; Matsumoto, Hideaki.

In: Physiologia Plantarum, Vol. 106, No. 1, 05.1999, p. 62-68.

Research output: Contribution to journalArticle

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abstract = "The effect of Aluminum (Al) on phytosiderophore-mediated solubilization of insoluble Fe and the uptake of phytosiderophore-Fe3+ complex was examined in wheat (Triticum aestivum L. cv. Atlas 66). Al addition did not affect the Fe solubilization by 2'-deoxymugineic acid (DMA), although Cu addition significantly inhibited the solubilization capacity. Addition of ten times more Al than Fe to the solution of DMA-Fe3+ complex did not decrease the absorption of the DMA-Fe3+ complex at 375 nm. Furthermore, NMR study indicated that Al did not shift the proton chemical shifts of DMA. All these results suggest that Al could not form a complex with the phytosiderophore, and is thereby unlikely to affect the process of phytosiderophore-mediated solubilization of Fe. Exposure of root to Al up to 100 μM for 3 h did not inhibit the DMA-Fe3+ uptake by the roots, but longer pretreatment (> 6 h) inhibited the uptake of the DMA-Fe3+ by more than 50{\%}. Neither the uptake of DMA-Fe3+ nor root elongation was inhibited by 24 h pretreatment with 10 μM Al, but both uptake and root elongation were inhibited by higher Al (> 20 μM) pretreatment. These results suggest that Al did not directly block the transport of the phytosiderophore-Fe3+ complex, and that the decreased uptake of the phytosiderophore-Fe3+ complex resulted from the roots being damaged by Al.",
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