Effect of activation treatments on actin filament distribution and in vitro development of miniature pig somatic cell nuclear transfer embryos

Ken Ichi Yamanaka, Satoshi Sugimura, Takuya Wakai, Takehisa Shoji, Jin Kobayashi, Hiroshi Sasada, Eimei Sato

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

In the present study, we investigated the effect of activation treatments on the actin filament distribution and in vitro development of somatic cell nuclear transfer (SCNT) embryos in miniature pigs. We combined three activation methods, ionomycin (ION), electrical stimulation (ES), and cycloheximide treatment (CH), to prepare seven activation treatments (ION, ES, CH, ION + CH, ION + ES, ES + CH and ION + ES + CH). First, we investigated the activation rate of oocytes and in vitro development of parthenotes. The activation rates of the oocytes in the ION, ES, CH, ION + CH, ION + ES, ES + CH, and ION + ES + CH groups were 42.9, 51.3, 0.0, 82.1, 80.6, 78.1 and 78.6%, respectively, showing that the rates of the combined treatment groups were significantly higher (P

Original languageEnglish
Pages (from-to)791-800
Number of pages10
JournalJournal of Reproduction and Development
Volume53
Issue number4
DOIs
Publication statusPublished - Aug 2007
Externally publishedYes

Fingerprint

miniature swine
Embryo Transfer
cycloheximide
embryo transfer
microfilaments
Actin Cytoskeleton
somatic cells
Ionomycin
Swine
Cycloheximide
Electric Stimulation
electrical treatment
Therapeutics
oocytes
Oocytes
In Vitro Techniques

Keywords

  • Actin filament
  • Activation
  • Nuclear transfer
  • Porcine oocyte

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Reproductive Medicine

Cite this

Effect of activation treatments on actin filament distribution and in vitro development of miniature pig somatic cell nuclear transfer embryos. / Yamanaka, Ken Ichi; Sugimura, Satoshi; Wakai, Takuya; Shoji, Takehisa; Kobayashi, Jin; Sasada, Hiroshi; Sato, Eimei.

In: Journal of Reproduction and Development, Vol. 53, No. 4, 08.2007, p. 791-800.

Research output: Contribution to journalArticle

Yamanaka, Ken Ichi ; Sugimura, Satoshi ; Wakai, Takuya ; Shoji, Takehisa ; Kobayashi, Jin ; Sasada, Hiroshi ; Sato, Eimei. / Effect of activation treatments on actin filament distribution and in vitro development of miniature pig somatic cell nuclear transfer embryos. In: Journal of Reproduction and Development. 2007 ; Vol. 53, No. 4. pp. 791-800.
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AU - Sasada, Hiroshi

AU - Sato, Eimei

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