Effect of accessory cells on stimulation of murine T-cell leukemia with antibodies to the CD3 T cell antigen receptor complex

Ryuichiro Suto, Heiichiro Udono, Akihito Yamamoto, Hiroshi Shiku, Eiichi Nakayama

Research output: Contribution to journalArticle

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Abstract

Stimulation of EL4 and RL♂1 leukemia cells in vitro with immobilized anti-CD3ε monoclonal antibody (mAb) (145-2C11) or anti-TCRβ mAb (H57-597) in the absence of accessory cells induced interleukin-2 (IL-2) production, and caused growth inhibition. The growth inhibition was, however, transient and the tumors started to grow again within 5 days in immobilizing plates treated with antibodies at concentrations of 2.5-100 μg/ml. Addition of mitomycin C-treated accessory cells to the culture inhibited IL-2 production and resulted in augmented and persistent growth inhibition. No recovery of tumor growth was observed. Furthermore, DNA from EL4 and RL♂1 leukemia cells stimulated with anti- CD3 TCR mAbs was fragmented even in the absence of accessory cells, but fragmentation was much greater in the presence of accessory cells. Marginal and high expression of the bcl-2 gene were observed in EL4 and RL♂1, respectively, indicating that apoptosis of these leukemias mediated by signalling through the CD3 TCR complex has no direct relationship with expression of the bcl-2 gene.

Original languageEnglish
Pages (from-to)438-444
Number of pages7
JournalJapanese Journal of Cancer Research
Volume84
Issue number4
Publication statusPublished - Apr 1993
Externally publishedYes

Fingerprint

T-Cell Antigen Receptor-CD3 Complex
T-Cell Leukemia
Antibodies
bcl-2 Genes
Leukemia
Growth
Interleukin-2
Monoclonal Antibodies
Mitomycin
Neoplasms
Cell Culture Techniques
Apoptosis
DNA

Keywords

  • Accessory cell
  • CD3 TCR stimulation
  • Murine T-cell leukemia

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Effect of accessory cells on stimulation of murine T-cell leukemia with antibodies to the CD3 T cell antigen receptor complex. / Suto, Ryuichiro; Udono, Heiichiro; Yamamoto, Akihito; Shiku, Hiroshi; Nakayama, Eiichi.

In: Japanese Journal of Cancer Research, Vol. 84, No. 4, 04.1993, p. 438-444.

Research output: Contribution to journalArticle

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AU - Suto, Ryuichiro

AU - Udono, Heiichiro

AU - Yamamoto, Akihito

AU - Shiku, Hiroshi

AU - Nakayama, Eiichi

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