Effect of a partial deletion of Y chromosome on in vitro fertilizing ability of mouse spermatozoa

M. Xian, S. Azuma, K. Naito, Tetsuo Kunieda, K. Moriwaki, Y. Toyoda

Research output: Contribution to journalArticle

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Abstract

The effect of a partial deletion of Y chromosome on sperm fertilizing ability was investigated through an in vitro fertilization technique. Epididymal spermatozoa of a congenic line, B10.BR-Y(del), which is characterized by a high incidence of abnormal spermatozoa, revealed a significantly lower in vitro fertilization rate (22%) than that (79%) of its control strain (B10.BR/SgSn), which has a normal-sized Y chromosome. Incidence of capacitated spermatozoa as determined by chlortetracycline fluorescence assay was significantly lower in B10.BR-Y(del) than in B10.BR/SgSn spermatozoa. The fertilization rate was significantly improved when B10.BR-Y(del) spermatozoa were separated from the supernatant of sperm suspension by Percoll gradient centrifugation. A reconstitution experiment revealed that the B10.BR-Y(del) spermatozoa were more sensitive to the inhibitory effect of the supernatant than B10.BR/SgSn spermatozoa. Spermatozoa from F1 (C57BL/6N male x B10.BR-Y(del) female) males showed higher fertilization rates than those from F1 (B10.BR-Y(del) male x C57BL/6N female) males. These observations suggest that not only the morphology but also the fertilizing ability of spermatozoa is directly related to partial deletion of Y chromosome.

Original languageEnglish
Pages (from-to)549-553
Number of pages5
JournalBiology of Reproduction
Volume47
Issue number4
Publication statusPublished - 1992
Externally publishedYes

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Spermatozoa
Fertilization in Vitro
Fertilization
Male sterility due to Y-chromosome deletions
In Vitro Techniques
Chlortetracycline
Y Chromosome
Incidence
Centrifugation
Suspensions
Fluorescence

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology
  • Embryology

Cite this

Effect of a partial deletion of Y chromosome on in vitro fertilizing ability of mouse spermatozoa. / Xian, M.; Azuma, S.; Naito, K.; Kunieda, Tetsuo; Moriwaki, K.; Toyoda, Y.

In: Biology of Reproduction, Vol. 47, No. 4, 1992, p. 549-553.

Research output: Contribution to journalArticle

Xian, M, Azuma, S, Naito, K, Kunieda, T, Moriwaki, K & Toyoda, Y 1992, 'Effect of a partial deletion of Y chromosome on in vitro fertilizing ability of mouse spermatozoa', Biology of Reproduction, vol. 47, no. 4, pp. 549-553.
Xian, M. ; Azuma, S. ; Naito, K. ; Kunieda, Tetsuo ; Moriwaki, K. ; Toyoda, Y. / Effect of a partial deletion of Y chromosome on in vitro fertilizing ability of mouse spermatozoa. In: Biology of Reproduction. 1992 ; Vol. 47, No. 4. pp. 549-553.
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