TY - JOUR
T1 - Effect of a partial deletion of Y chromosome on in vitro fertilizing ability of mouse spermatozoa
AU - Xian, M.
AU - Azuma, S.
AU - Naito, K.
AU - Kunieda, T.
AU - Moriwaki, K.
AU - Toyoda, Y.
PY - 1992
Y1 - 1992
N2 - The effect of a partial deletion of Y chromosome on sperm fertilizing ability was investigated through an in vitro fertilization technique. Epididymal spermatozoa of a congenic line, B10.BR-Y(del), which is characterized by a high incidence of abnormal spermatozoa, revealed a significantly lower in vitro fertilization rate (22%) than that (79%) of its control strain (B10.BR/SgSn), which has a normal-sized Y chromosome. Incidence of capacitated spermatozoa as determined by chlortetracycline fluorescence assay was significantly lower in B10.BR-Y(del) than in B10.BR/SgSn spermatozoa. The fertilization rate was significantly improved when B10.BR-Y(del) spermatozoa were separated from the supernatant of sperm suspension by Percoll gradient centrifugation. A reconstitution experiment revealed that the B10.BR-Y(del) spermatozoa were more sensitive to the inhibitory effect of the supernatant than B10.BR/SgSn spermatozoa. Spermatozoa from F1 (C57BL/6N male x B10.BR-Y(del) female) males showed higher fertilization rates than those from F1 (B10.BR-Y(del) male x C57BL/6N female) males. These observations suggest that not only the morphology but also the fertilizing ability of spermatozoa is directly related to partial deletion of Y chromosome.
AB - The effect of a partial deletion of Y chromosome on sperm fertilizing ability was investigated through an in vitro fertilization technique. Epididymal spermatozoa of a congenic line, B10.BR-Y(del), which is characterized by a high incidence of abnormal spermatozoa, revealed a significantly lower in vitro fertilization rate (22%) than that (79%) of its control strain (B10.BR/SgSn), which has a normal-sized Y chromosome. Incidence of capacitated spermatozoa as determined by chlortetracycline fluorescence assay was significantly lower in B10.BR-Y(del) than in B10.BR/SgSn spermatozoa. The fertilization rate was significantly improved when B10.BR-Y(del) spermatozoa were separated from the supernatant of sperm suspension by Percoll gradient centrifugation. A reconstitution experiment revealed that the B10.BR-Y(del) spermatozoa were more sensitive to the inhibitory effect of the supernatant than B10.BR/SgSn spermatozoa. Spermatozoa from F1 (C57BL/6N male x B10.BR-Y(del) female) males showed higher fertilization rates than those from F1 (B10.BR-Y(del) male x C57BL/6N female) males. These observations suggest that not only the morphology but also the fertilizing ability of spermatozoa is directly related to partial deletion of Y chromosome.
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U2 - 10.1095/biolreprod47.4.549
DO - 10.1095/biolreprod47.4.549
M3 - Article
C2 - 1327209
AN - SCOPUS:0026784549
SN - 0006-3363
VL - 47
SP - 549
EP - 553
JO - Biology of Reproduction
JF - Biology of Reproduction
IS - 4
ER -